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Supramolecular hydrogel gene vector material, and preparation method and application thereof

A supramolecular hydrogel and gene carrier technology, applied in the field of biomedical engineering materials, can solve the problem that the application of supramolecular hydrogel has not been reported yet, and achieve convenient and fast application, good biocompatibility, and good shear thinning. Effect

Inactive Publication Date: 2011-06-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, supramolecular hydrogels and their applications for in situ gene loading via host-guest interactions between polymers and cyclodextrins have not been reported

Method used

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  • Supramolecular hydrogel gene vector material, and preparation method and application thereof
  • Supramolecular hydrogel gene vector material, and preparation method and application thereof
  • Supramolecular hydrogel gene vector material, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] a. Dissolve the dried polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer Pluronic F-127 in a mixed solution of dichloromethane and pyridine, then add p-toluenesulfonyl chloride at 35°C Under room temperature conditions, the reaction was protected from light for 24 hours; the molar ratio of the polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer to p-toluenesulfonyl chloride was 1:2, and the solvent dichloromethane and pyridine The volume ratio of the mixture is 2: 1, containing 15 grams of Pluronic F-127 in every 100 milliliters of mixed solutions; The amount added is 3 grams per 100 milliliters of organic phase), washed and filtered; dichloromethane was evaporated to obtain polyethylene glycol-polypropylene glycol-polyethylene glycol-p-toluenesulfonate;

[0044] B, the ammonia solution (polyethylene glycol-polypropylene glycol-polyethylene glycol-p-toluenesulfonate) of above-mentioned product Polyethylene Glycol-...

Embodiment 2

[0049] a. Dissolve the dried polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer Pluronic F-68 in a mixed solution of dichloromethane and pyridine, then add p-toluenesulfonyl chloride, at 15°C Under room temperature conditions, the reaction was protected from light for 36 hours; the molar ratio of the polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer to p-toluenesulfonyl chloride was 1:2, and the solvent dichloromethane and pyridine The volume ratio is 2: 1, containing 5 grams of Pluronic F-68 in every 100 milliliters of mixed solutions; The amount added is 5 grams per 100 milliliters of organic phase), washed and filtered; dichloromethane was evaporated to obtain polyethylene glycol-polypropylene glycol-polyethylene glycol-p-toluenesulfonate;

[0050] B, the ammonia solution (polyethylene glycol-polypropylene glycol-polyethylene glycol-p-toluenesulfonate) of above-mentioned product Polyethylene Glycol-Polypropylene Gly...

Embodiment 3

[0055] (1) At room temperature at 25°C, the polyethylene glycol-polypropylene glycol-polyethylene glycol-polylysine multi-block polymer obtained in Example 2 was formulated into 1 mL with a mass percentage concentration of 8% (w / w), and then add a plasmid pEGFP solution with a concentration of 20 μL 0.5 μg / μL (purchased from Invitrogen and amplified with Escherichia coli ) to it, mix well and let stand for 20 minutes to obtain a polymer / pEGFP complex solution;

[0056] (2) Add 1.0 mL of 15% α-cyclodextrin aqueous solution to the polymer / pEGFP complex solution obtained in step (1), stir and mix evenly, and let stand at room temperature for 6 hours to obtain supramolecular Hydrogel gene carrier material B.

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Abstract

The invention discloses a supramolecular structural hydrogel gene vector material and a preparation method and application thereof. The method comprises the following operation steps: synthesizing a multi-block copolymer with cation chain segments through modification of a terminal group of triblock copolymer of a polyethylene glycol-polypropylene glycol-polyethylene glycol which is modified by polylysine; then, mixing the copolymer with DNA solution to obtain DNA compound micelle; further, mixing and then stirring the micelle solution and solution of alpha-cyclodextrin; and keeping mixed solution at the room temperature to obtain hydrogel. The hydrogel can be used for preparing an injectable gene vector. The method has the advantages that the method has simple operation, can adjust the strength of the hydrogel and the gelatination time, can mold at the room temperature and does not relate to chemical crosslinking reaction and the use of organic solvent; and the obtained hydrogel has the advantages of temperature sensitivity, good bio-compatibility, obvious transfection effect and the like; and the method is hopeful to be widely applied in the field of a biomedical engineering material.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, and in particular relates to a supramolecular hydrogel which can be used as a gene carrier and its preparation method and application. Background technique [0002] Gene therapy is an effective method currently used in the treatment of cancer and innate immune system diseases. The key to the implementation of this technology is to select the appropriate gene carrier and gene introduction method, so that the gene can be expressed in cells safely, efficiently, controllably and stably. As a class of biomedical materials that can be used as drug carriers, hydrogels have the characteristics of high loading rate, protection of macromolecular drugs from degradation or removal by organisms, and sustainable release of drug molecules. Research for gene loading and controlled release. For example, Park et al. used double-bond-modified polyethylene glycol-polypropylene glycol-polyethylene gl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/40A61K47/34A61K48/00A61P35/00A61P37/02
Inventor 马栋张黎明张宏斌李娜杨川严励
Owner SUN YAT SEN UNIV
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