Quick analyzing method for glutelin content of wheat
A rapid analysis, gluten protein technology, applied in the field of grain chemistry, can solve the problems of complex operation, low work efficiency, high cost, etc., and achieve the effect of simple operation method, high extraction speed and high strength effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Among the wheat samples to be tested, 20 g of representative grains after cleaning were selected, and the whole wheat flour was ground by FOSSCyclotec 1093 experimental cyclone. Stir well with a medicine spoon and seal the bag, and leave it at room temperature for more than 24 hours until the water content is uniform. According to GB 5497-85, the water content of the whole wheat flour sample is determined according to the method of grain oil detection-moisture determination, and it is used as the sample to be tested.
Embodiment 2
[0029] a. Biuret reagent preparation:
[0030] Prepare 4% CuSO at room temperature according to the amount required for the assay sample 4 (w / v), 2.5% sodium potassium tartrate (w / v) and 5M NaOH solution. Add 30mL of CuSO to a 500mL volumetric flask 4 Solution, 100mL potassium sodium tartrate solution, then slowly add 30mL NaOH solution, and finally distilled water to 500mL to form a spare solution;
[0031] Before starting to measure the sample to be tested, take an appropriate amount of the stock solution and mix it with an equal volume of isopropanol to form a biuret reagent.
[0032] b. SDS phosphate buffer preparation:
[0033] Solution 1: 0.05mol / L Na 2 HPO 4 , 0.5% SDS (w / v);
[0034] Solution 2: 0.05mol / L NaH 2 PO 4 , 0.5% SDS (w / v);
[0035] Take an appropriate amount of solution 1 in a beaker, slowly add solution 2, and continue to stir until the pH of the mixed solution drops to 6.90, which is the SDS phosphate buffer solution.
Embodiment 3
[0037] Standard curve drawing:
[0038] Dilute protein standard solution (7g / dL) with 0.1mol / L NaOH solution to 5mg / mL standard solution. Take protein standard solution (5mg / mL) 0.0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0mL in 10mL test tubes, add 1.0, 0.9, 0.8, 0.7, 0.6, 0.5 , 0.4, 0.3, 0.2, 0.1, 0.0ml of distilled water, add 4.0mL biuret reagent (provided in a in Example 2, the same below) to each test tube, mix well, 40 ℃ water bath 20min, 560nm wavelength colorimetry, measure the absorbance value. Take the absorbance value as the abscissa, and take the protein content as the ordinate to make a standard curve, and obtain the following one-variable linear equation:
[0039] y=12.516x-0.0316
[0040] In the formula: x is the absorbance value, y is the percentage of protein, and in this embodiment, their correlation coefficient r=0.9999.
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More