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Biological chip for synchronously detecting typhoid and paratyphoid and preparation method thereof

A biochip, paratyphoid technology, applied in measurement devices, resistance to vector-borne diseases, instruments, etc., can solve the problems of time-consuming and labor-intensive detection efficiency, and can not be detected at the same time, and achieve the effect of saving time.

Inactive Publication Date: 2010-06-16
深圳市赛尔生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The above methods have their own advantages and disadvantages, but they all need one experiment to detect only one type of bacteria, or one antibody, and cannot detect typhoid O, H, VI and paratyphoid A, B, C at the same time
It is time-consuming and labor-intensive in terms of detection efficiency, especially the traditional Widal reaction takes 12-24 hours, and isolation and culture takes 3-5 days
Although the PCR method is sensitive and specific, it takes several hours and requires expensive instruments. One experiment can only diagnose one type of bacteria, which cannot be implemented in primary medical institutions because it requires a strictly controlled operating space and clean environment.

Method used

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  • Biological chip for synchronously detecting typhoid and paratyphoid and preparation method thereof
  • Biological chip for synchronously detecting typhoid and paratyphoid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Preparation of Biochip for Simultaneous Detection of Typhoid and Paratyphoid

[0036] Such as figure 1 As shown, a biochip for simultaneously detecting typhoid fever and paratyphoid fever includes a poly-L-lysine-activated glass three-dimensional chip substrate, and the substrate is provided with at least one detection unit, and the detection unit is provided with a detection unit. The dot matrix of samples to be inspected is a 4×5 double-site design; from top to bottom, A, B, C, and D represent rows respectively, and from left to right use numbers 1, 2, 3, 4, 5 represent columns, respectively. Among them, each site A1, B1, C1, and D1 represents the quality control point; each site: A2, B2 represents the typhoid O site; A3, B3 represents the typhoid H site; A4, B4 represents the typhoid VI site; A5, B5 C2 and D2 represent paratyphoid B loci; C3 and D3 represent paratyphoid C loci; C4 and D4 represent negative control (NC) loci; C5 and D5 are blank control loci. The a...

Embodiment 2

[0044] Assembly of the assay kit

[0045] 1. The biochip 2TX10 / 20 described in embodiment 1

[0046] 2. 1 bottle of specimen diluent (2.5 / 5ml, blue)

[0047] 3. 1 bottle of tracer (2.5 / 5ml, red)

[0048] 4. Chromogenic agent 1 bottle

[0049] 5. 2 / 4 bottle of washing liquid (18mlX2 / 4, used directly)

[0050] 6. Instruction manual 1 piece

[0051] 7. Bring your own wet box: put a layer of wet gauze in the lunch box.

Embodiment 3

[0053] Simultaneous detection of Salmonella typhi infection by detection kit

[0054] The kit described in Example 2 was equilibrated to room temperature. Specimen dilution: Dilute the serum specimen 1:20 (15ul~300ul) with the specimen diluent and mix well in the test tube. Adding samples: Take out the chip and unpack it and place it flat on the test bench, add 200ul of diluted samples to each grid, and make sure that the samples cover the grid. Incubation: Place the chip in a wet box and incubate at 37°C for 30 minutes. Shake off the liquid in the grid, add 4 drops of washing liquid to each grid, shake it off immediately, wash 3 times in a row, and finally wash with distilled water for 1 second, and then dry the water droplets around the grid on absorbent paper. Add tracer: drop 2 drops of tracer per grid, do not overflow the grid. Incubate in a wet box at 37°C for 30 minutes, wash 3 times as above, and blot the surrounding water droplets on absorbent paper. Color develop...

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Abstract

The invention discloses a biological chip for synchronously detecting typhoid and paratyphoid, which belongs to the technical field of biological chips and comprises a chip substrate. The substrate is provided with at least one detection unit, the detection unit is provided with a detection sample dot matrix to be detected, and the detection dot matrix has a double site design; the detection dot matrix comprises a quality control detection point, a negative control detection point, typhoid O, H and VI type detection sites and paratyphoid A, B and C type detection sites; and the quality control detection point, the negative control detection point, the typhoid O, H and VI type detection sites and the paratyphoid A, B and C type detection sites are coated with corresponding antigens respectively. When the biological chip is used for detecting, the efficient, time-saving and high-pass synchronous diagnosis and detection for typhoidal salmonellosis can be realized, namely the aim of acquiring the combination of typhoid O, H and VI types and paratyphoid A, B and C types through once detection is fulfilled.

Description

technical field [0001] The invention belongs to the technical field of biochips, and in particular relates to a biochip for simultaneous detection of typhoid and paratyphoid, a preparation method thereof, and a detection kit comprising the chip. Background technique [0002] Intestinal infection is the third leading cause of disease in the world, and enteric fever caused by Salmonella typhi and Salmonella paratyphi A accounts for the main part. This disease composition is more pronounced in countries with poor sanitation and untreated drinking water, and is a major problem threatening people's health. In Asia, Salmonella paratyphi A has become a major cause of enteric fever. Salmonella paratyphi A was isolated in China in 1998, and it has gradually become the prevalent dominant strain, often causing endemic epidemics and outbreaks. [0003] Typhoid fever and paratyphoid fever are widely distributed in the world. In 2000, the World Health Organization (WHO) estimated that t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCY02A50/30
Inventor 林连成
Owner 深圳市赛尔生物技术有限公司
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