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Dry enzyme method biochip/test strip

A technology of biochips and test strips, applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of siphon failure, damage, loss of water absorption function, etc.

Inactive Publication Date: 2010-06-30
张祥成
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Otherwise PET cannot be used as a siphon cover slip
Another problem is that PET is a specially processed water-absorbing chemical substance (coating) that is easily deteriorated and damaged. Especially under high temperature and high humidity, it quickly loses its water absorption function
In addition, when the test solution is injected into the end (such as finger blood into the end of the blood glucose meter test piece), a vent hole must be designed at the rear end of the sample injection. to siphon

Method used

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  • Dry enzyme method biochip/test strip
  • Dry enzyme method biochip/test strip
  • Dry enzyme method biochip/test strip

Examples

Experimental program
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Embodiment Construction

[0024] as the picture shows:

[0025] [1] PET: width 7mm, thickness 0.2mm; length 37mm;

[0026] [2] Graphite is printed first, width 1mm, thickness 0.005-0.015mm; on it, printing Ag / AgCl reference electrode material: width 0.5mm, thickness 0.005-0.015mm, and the area of ​​dry enzyme electrode is not printed with silver And silver chloride composite material, the thickness is between 5-20μm;

[0027] [3] It is a dry enzyme system screen printed on the carbon electrode: width 0.75-1.5mm, thickness 0.002-0.01mm;

[0028] [4] Hydrophilic omentum: the basic material of omentum is PET fiber, the fiber diameter is 0.005mm, and the fiber line spacing is 0.15mm;

[0029] [5] is a printed hydrophobic insulating varnish.

[0030] Among them, the conductive carbon paste, Ag / AgCl reference electrode paste and dry enzyme system produced by Cambridge NanoTech Limited (Yingjian Nano Technology Company) are used. The test results of the cholesterol biochip / test strip thus produced are as follows Figu...

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PUM

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Abstract

The invention relates to a biological sensing chip, which is applicable to the fast diction of various indexes in the blood, including the indexes of cholesterin, triolein, blood sugar protein, blood sugar, lactic acid, uric acid, blood pH, blood oxygen and the like. The invention particularly relates to a dry enzyme method biochip / test strip, which belongs to the technical field of fast detection of a medical miniature biosensor. The dry enzyme method biochip comprises a base sheet, a dry enzyme work electrode, a reference electrode, a test liquid diffusing reticular film and hydrophobic insulation paint, wherein the dry enzyme work electrode and the reference electrode are 10 nm to 30 mum higher than the base sheet, the test liquid diffusing reticular film has the hydrophilicity, the contact angle of the test liquid diffusing reticular film and the pure water is approximately zero degree, and the test liquid including whole blood, blood serum, blood plasma or other tested liquid can be uniformly permeated and diffused onto the dry enzyme work electrode through the reticular film, so various blood parameters or other organic and inorganic liquid parameters can be tested by an electrochemical method.

Description

Technical field [0001] The invention relates to a biological sensor chip, which is suitable for quickly detecting multiple indexes in the blood, including: cholesterol, triglyceride, blood glucose protein, blood sugar, lactic acid, uric acid, blood pH, blood oxygen and other indexes, and is also suitable for Various other instruments, such as quantitative and qualitative analysis instruments in the fields of biology, chemistry, food, environmental protection, etc., specifically relate to a dry enzyme biochip / test strip, which belongs to the technical field of rapid detection of medical micro-biosensors. Background technique [0002] Mini rapid medical analysis instrument is a high-tech industry that has emerged in recent years, and its core technology is molecular and biomolecular sensing technology. A biosensor is a detector that uses biologically active units (such as enzymes, antibodies, nucleic acids, cells, etc.) as a biologically sensitive unit and is highly selective for t...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 张祥成
Owner 张祥成
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