Method for extracting athomin with mustard seeds as raw material

A mustard seed and horseradish technology, applied in the direction of fermentation, etc., can solve the problems of low yield, long time consumption of allyl isothiocyanate, low extraction rate, etc., and achieve short reaction time and outstanding substantive characteristics , the effect of high product content

Inactive Publication Date: 2010-07-07
北京农鹰现代农业技术研究院 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of producing allyl isothiocyanate by this method takes a long time, the extraction rate is low, and the yield is not high

Method used

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  • Method for extracting athomin with mustard seeds as raw material
  • Method for extracting athomin with mustard seeds as raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The method for extracting horseradish as raw material with mustard seeds of the present invention comprises the following steps:

[0017] A, prepare raw mustard seeds, and crush it to 20-40 mesh;

[0018] B. Add the crushed mustard seeds into the reaction kettle, soak the crushed mustard seeds for 1-4 hours with pH 4-6.5, temperature 30-80°C acidic aqueous solution, wherein the optimum temperature of the acidic aqueous solution is 50 DEG C, soak for 2 hours, the water for soaking the mustard seeds is preferably deionized water, adopt the existing technology to catalyze the glucosinolates (Thioglucosides) contained in the mustard seeds under the catalysis of glucosinolate enzyme (Myrosinase) Hydrolyzed to allyl isothiocyanate, wherein the acid regulator of the aqueous solution is acetic acid or lactic acid or malic acid or citric acid or gluconic acid or tartaric acid or fumaric acid or ascorbic acid, the weight ratio of mustard seed to water is 1:2 or 1 :2.5 or 1:3 or ...

Embodiment 2

[0022] Using mustard seeds as processing raw materials, first crush the mustard seeds into 20-40 meshes, then add to the reaction kettle, then add deionized water, the weight ratio of mustard seeds to deionized water is 1:6, adjust the deionized water with acetic acid The pH of the ionized water is pH4.5, and the temperature of the water is 40°C. The mustard seeds are soaked in the aqueous solution for 3 hours, so that the glucosinolates (Thioglucosides) contained in the mustard seeds are activated by the glucosinolate enzyme (Myrosinase). ) under catalysis to be hydrolyzed into allyl isothiocyanate, and then extract the product in the reaction kettle by subcritical water extraction at 1Mpa and 100°C for 50 minutes, and then separate the product in the reaction kettle to obtain crude horseradish. The content of allyl isothiocyanate in the detected product was 68.6%, and the oil yield was 1.9%.

Embodiment 3

[0024] Using mustard seeds as processing raw materials, first crush the mustard seeds into 20-40 meshes, then add to the reaction kettle, then add deionized water, the weight ratio of mustard seeds to deionized water is 1:4, adjust with citric acid The pH of the deionized water is pH 5, and the temperature of the water is 50°C. Soak the mustard seeds with the aqueous solution for 2 hours, so that the glucosinolates (Thioglucosides) contained in the raw materials are catalyzed by glucosinolate enzyme (Myrosinase). Hydrolyze to allyl isothiocyanate, then extract the product in the reaction kettle by subcritical water extraction at 2Mpa and 120°C for 40 minutes, and then separate the product in the reaction kettle to obtain crude horseradish. The content of allyl isothiocyanate is 68.2%, and the oil yield is 2.2%.

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Abstract

The invention relates to a method for extracting athomin with mustard seeds as the raw material. The method comprises the following steps: taking mustard seeds in brassica in cruciferae as the processing raw material, soaking the raw material in water after being crushed, and then rapidly and effectively obtaining crude product of athomin by a subcritical water extraction method. Because the subcritical water extraction technology is a novel environment-friendly extraction technology not using or rarely using organic solvent, when being in the subcritical state, water has certain dissolving capacity to semi-polar and non-polar organisms, the polarity, the surface tension and the viscosity of water can be changed by controlling the temperature and the pressure of subcritical water, so that the dissolving capacity of the subcritical water to the organisms can be greatly imoroved, therefore, the extraction efficiency is greatly improved, and the content of allyl isothiocyanate product in athomin is high. Compared with the prior art, the method has the advantages of simple technological line, reasonable technological condition, simple and safe reaction and operation, short reaction time, low solvent loss, low technological cost, and high implementation value and social and economic benefit.

Description

technical field [0001] The invention relates to a method for extracting horseradish from mustard seeds. Background technique [0002] Allyl isothiocyanate (AITC) is the main component of horseradish, which can be used as flavoring agent, storage preservative, insecticide, fungicide, fine chemical intermediate and medical supplies, in food, chemical, pesticide and It is widely used in medicine and other industries. At present, the methods for preparing allyl isothiocyanate mainly include chemical industrial synthesis and biological extraction. Among them, the biological extraction method has an irreplaceable position as people pay more and more attention to food safety and industrial production environmental issues. [0003] The biological extraction method of allyl isothiocyanate is based on plants such as brassica mustard seed, horseradish and behenicum as processing raw materials, and the glucosinolates (Thioglucosides) contained in the raw materials are Under the catal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P11/00C12P1/00
Inventor 郑建秋李健强石尚柏曹永松卢志军刘西莉郑翔罗来鑫刘鹏飞郑炜
Owner 北京农鹰现代农业技术研究院
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