Bast fiber pectase degumming method
The technology of bast fiber and degumming method is applied in the field of biological degumming process of bast fiber. Effect of improving fiber dispersibility
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Embodiment 1
[0021] A kind of bast fiber pectinase degumming method, it comprises pectinase liquid preparation and following steps:
[0022] (1) pretreatment of bast fiber (the present embodiment is ramie);
[0023] (2) One-time enzymatic degumming: prepare enzyme liquid, control enzyme liquid activity 400u / ml-1000u / ml (this embodiment is 800u / ml), adjust enzyme liquid pH value 7-10 (this embodiment is 8.5), enzyme Liquid is warmed up to 40 ℃-70 ℃ (the present embodiment is 55 ℃), by mass ratio, bast fiber and enzyme liquid ratio are 1: 9-15 (the present embodiment is 1: 12), bast fiber is in this Dip enzyme under condition 2 hours-3 hours (the present embodiment is 2.5 hours);
[0024] (3) Secondary enzymatic degumming: take out the bast fiber obtained in step (2), and fully rinse it with hot water at 50°C-90°C (80°C in this embodiment), and wash off the bast fiber attached Colloid, repeat step (2);
[0025] (4) post-processing.
[0026] In step (1) of the present invention, the bast ...
Embodiment 2
[0029] The preparation of the pectin enzyme liquid of the present invention includes strain selection, strain cultivation, shake flask fermentation and preparation of the enzyme liquid.
[0030] The strain selection is based on methods known in the art, through natural collection of bacterial samples in the soil of the main producing areas of hemp plants such as Hunan, Sichuan, Henan, etc., and one strain is obtained from nearly a hundred strains through shaking flask screening. strains, and at the cell level, nitrosoguanidine and ultraviolet radiation were used to mutagenize nearly ten thousand strains, and a strain with the laboratory number MAPLE21 was obtained after shake flask screening, namely Bacillus cereus (Bacillus cereus), whose biological The chemical characteristics are: the bacteria are rod-shaped, evenly stained, G + , Facultatively aerobic, forming spores, the spores do not protrude from the thallus, the ends of the thallus are relatively flat, and most of them...
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