Detection method of human plasma surface enhanced raman spectroscopy by integrating main component analysis

A surface-enhanced Raman and principal component analysis technology, applied in the field of biomedicine, can solve the problems of sample damage, strong autofluorescence interference, weak spectral signal, etc., to achieve the effect of non-destructive testing

Inactive Publication Date: 2010-08-18
FUJIAN NORMAL UNIV +1
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Problems solved by technology

The shortcomings of these studies are that conventional Raman spectroscopy signals are weak, autofluorescence interference is strong, and conventional laser Raman detection requires a large excitation light power and takes a long time, which is easy to cause damage to the sample.
However, the use of non-polarized laser, linearly polarized laser or circularly polarized laser as excitation light, silver colloid as the reinforcement matrix surface-enhanced Raman spectroscopy combined with principal component analysis to detect and analyze human plasma has not yet been reported.

Method used

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  • Detection method of human plasma surface enhanced raman spectroscopy by integrating main component analysis
  • Detection method of human plasma surface enhanced raman spectroscopy by integrating main component analysis
  • Detection method of human plasma surface enhanced raman spectroscopy by integrating main component analysis

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Experimental program
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Effect test

Embodiment 1

[0057] The overnight fasting blood of two groups of different people between 7:00 and 8:00 am was drawn, EDTA was added to prevent blood coagulation, and centrifuged (2000 rpm) for 15 minutes. The upper layer of serum was discarded, and the lower layer of plasma was taken as a sample. The first group of human plasma totaled 33 copies, which were compiled as group A. The second group consisted of 43 samples of human plasma, which were grouped as group B; 200 μl of each sample plasma was taken from group A with a pipette gun and added to a sterile test tube. And use a pipette gun to add 200 μl of the previously prepared centrifuged silver sol into the test tube, and mix according to the volume ratio of plasma and silver sol at 1:1. The mixed solution was fully stirred, so that the plasma and the silver sol were mixed as evenly as possible, and a group A plasma-silver sol mixed solution was prepared. Use a pipette to take out 200 μl of each sample plasma from group B and add it...

Embodiment 2

[0062] Take overnight fasting blood from two different people between 7:00 and 8:00 in the morning, add EDTA to prevent blood coagulation and centrifuge (2000 rpm) for 15 minutes. The upper layer of serum was discarded, and the lower layer of plasma was taken as a sample. Use a pipette gun to take 200 μl of sample plasma from the first plasma sample and add it to a sterile test tube. And use a pipette gun to add 200 μl of the previously prepared centrifuged silver sol into the test tube, and mix according to the volume ratio of plasma and silver sol at 1:1. The mixed solution was fully stirred, so that the plasma and the silver sol were mixed as evenly as possible, and the first plasma-silver sol mixed solution was prepared. Use a pipette to take out 200 μl of sample plasma from the second human plasma and add it to a sterile test tube. And use a pipette to add 200 μl of the previously prepared centrifuged silver sol into the test tube, and mix according to the volume ratio ...

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Abstract

The invention provides a detection method of human plasma surface enhanced raman spectroscopy by integrating main component analysis; the method comprises the following steps that: a human plasma sample in physiological conditions is obtained, and is reduced by hydroxylamine hydrochloride to prepare silver sol; the silver sol and the human plasma sample are uniformly mixed by equal volume and incubated for 2h at 4DEG C, to measure the plasma surface enhanced raman spectroscopy; the measurement to the plasma surface enhanced raman spectroscopy can also adopt laser in different polarization states to excite the human plasma sample; and a plasma surface enhanced raman spectroscopy database is built, and the scattergraph distribution corresponding to the surface enhanced raman spectroscopy of different human plasma is obtained by integrating main component analysis. The detection method ensures the activity of biomolecules in the plasma, and has the advantages of simplicity, fastness and strong reliability. In addition, an SERS technology enables the sample to obtain very strong raman spectroscopy signals at very low laser power, so that the repeatability of the spectrum signals is good, thereby preventing carbonation and damage of high-power laser to the biological sample.

Description

technical field [0001] The invention relates to a human plasma surface-enhanced Raman spectrum combined with multivariate analysis and detection method, more specifically, human blood is subjected to plasma centrifugation under aseptic conditions to obtain a human plasma solution in a physiological state, and then the human plasma solution is obtained by surface enhancement. Raman spectroscopy is used to detect the SERS signal of plasma, and a method for statistical analysis using multivariate analysis techniques. Belongs to the field of biomedicine. Background technique [0002] Raman spectroscopy technology can provide molecular vibration spectra, which contain molecular fine structure information and characteristic fingerprints. Raman spectroscopy has become one of the important technologies for item identification and molecular detection. The application of Raman spectroscopy technology to the research of life sciences can analyze the structure or components of human ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 冯尚源陈荣林居强陈冠楠李永增黄祖芳陈杰斯曾海山
Owner FUJIAN NORMAL UNIV
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