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Method for preparing high-purity gardenoside

A high-purity geniposide technology, applied in the field of preparation of high-purity geniposide, can solve problems such as low yield, excessive material consumption, and increased consumption of macroporous resins, shorten extraction time, reduce production costs, increase The effect of the amount of adsorption

Inactive Publication Date: 2010-11-24
GUANGXI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preliminary conclusion drawn through comparative research is that the above-mentioned first three methods are acceptable as research, but cannot be used for industrial production
Its main problems are: complex process, excessive material consumption, low yield, and the use of toxic and harmful reagents in large quantities, such as ether, chloroform, methanol, acetone, lead salt, etc.
In this process, since the crude extract contains a large amount of carbohydrates, gardenia xanthin and other impurities, the amount of macroporous resin will be greatly increased and the regeneration of the resin will be difficult. Not conducive to industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 500g of dried gardenia fruit powder, ultrasonically extract three times at 50°C, add 5000ml of distilled water for extraction for 1 hour each time, combine the three extractions and concentrate under reduced pressure to form 120ml of dilute extract, which is precipitated when added to 500ml of 95% ethanol A large amount of sugar precipitates, filters, and the filtrate is concentrated under reduced pressure into 90 g of extract, which is dissolved in 200 ml of distilled water, extracted continuously for 5 times with 200 ml of methyl acetate, and the combined extracts are concentrated under reduced pressure for 25 g of enriched extract. Dilute the extract with 1000ml of distilled water and put it on the pretreated DM130 macroporous resin column. After the adsorption is saturated, wash the column with 8 times of column volume of distilled water, discard the effluent, and then wash with 5 times of column volume of 20% ethanol aqueous solution. After elution, the eluent...

Embodiment 2

[0024] Weigh 500g of dried gardenia fruit powder, ultrasonically extract three times at 50°C, add 5000ml of distilled water for extraction for 1 hour each time, combine the three extractions and concentrate under reduced pressure to form 120ml of dilute extract, which is precipitated when added to 500ml of 95% ethanol A large number of sugars precipitated, filtered, and the filtrate was concentrated under reduced pressure into 95g of extract, which was dissolved in 200ml of distilled water, extracted 8 times with 200ml of ethyl formate, and the extracts combined 8 times were concentrated under reduced pressure into 30g of extract, and 1000ml of extract was used After diluting with distilled water, put it on the pretreated DM130 macroporous resin column. After the adsorption is saturated, wash the column with distilled water of 8 times the column volume, discard the effluent, and then elute with 20% ethanol aqueous solution of 5 times the column volume. Concentrate under reduced...

Embodiment 3

[0026] Weigh 500g of dried gardenia fruit powder, ultrasonically extract four times at 65°C, add 5000ml of distilled water for extraction for 1 hour each time, combine the four extractions and concentrate under reduced pressure to form 150ml of dilute extract, which is added to 600ml of 95% ethanol Promptly separate out a large amount of sugar precipitates, filter, and the filtrate is concentrated under reduced pressure into 110g of extractum, extractum is dissolved in 150ml distilled water, extracts 9 times with 200ml methyl acetate, and the extracts of merging 9 times are concentrated under reduced pressure into 34g of extractum, extractum After diluting with 800ml of distilled water, put it on the pretreated D101 macroporous resin column. After the adsorption is saturated, wash the column with distilled water of 8 times the column volume, discard the effluent, and then elute with 15% aqueous ethanol solution of 6 times the column volume. The crude crystals of geniposide were...

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Abstract

The invention discloses a method for preparing high-purity gardenoside. The method comprises the following steps of: extracting and concentrating gardenia fruits serving as raw materials with water, and removing a great number of saccharide substances by an alcohol precipitation method; then extracting the raw materials with an appropriate single organic solvent; performing preliminary enrichment on gardenoside; finally absorbing and desorbing macroporous resin on gardenoside liquor after the preliminary enrichment to obtain gardenoside coarse crystals; and recrystallizing the gardenoside coarse crystals to obtain high-purity products. The method has the following advantages that: 1, the extraction method is fast and thorough; 2, the water extract is precipitated by ethanol to make the extraction operation have no emulsification phenomena so as to greatly shorten extraction time; and 3, the single solvent for extracting is convenient to recycle in industrial production so as to greatly reduce the production cost.

Description

technical field [0001] The invention relates to a method for preparing chemical products, in particular to a method for preparing high-purity geniposide. technical background [0002] Gardenia is the dry and mature fruit of Gardenia jasminoides of the Rubiaceae plant, oblong or oval, dark red or yellow-red in color. "Compendium of Materia Medica" has detailed records: Gardenia has the functions of promoting gallbladder and protecting liver, clearing heat and detoxification, lowering blood pressure, antibacterial and anti-inflammatory. Traditional Chinese medicine is used clinically to treat jaundice hepatitis, sprains, high blood pressure, diabetes, etc. There are many chemical components in gardenia, and its main components are iridoids such as crocetin, crocetin and geniposide. Geniposide has many uses. After fermentation under different conditions, it can be made into natural food colorants gardenia blue and gardenia red. It is also a raw material drug for treating card...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 韦万兴卓家雄朱建文周敏朱梅
Owner GUANGXI UNIV
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