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Preparation method of pirimicarb molecularly imprinted polymer

A molecular imprinting and polymer technology, applied in chemical instruments and methods, other chemical processes, etc., can solve the problems of staying in the evaluation stage of the physical characteristics and adsorption properties of polymers, difficult elution of pirimicarb, etc., and achieve large-scale promotion. Application value, improving detection sensitivity and detection efficiency, and the effect of efficient enrichment

Inactive Publication Date: 2010-11-24
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Find through the retrieval of prior art document, the article " Piezoelectric quartz crystal sensor for rapid analysis of pirimicarb residues using molecularly imprinted polymers as recognition elements " (using molecularly imprinted polymers as recognition elements " published on " Analytica Chimica Acta " 2006,576,67-76 by Fung etc. Piezoelectric quartz crystal sensor with imprinted polymer as identification material for rapid detection of pirimicarb residue) synthesized anti-pirimicarb molecularly imprinted polymer; Pingarrón et al. The article "Molecularly imprinted polymers for on-line preconcentration by solid phase extraction of pirimicarb inwater samples" also synthesized pirimicarb molecularly imprinted polymers; However, the polymerization method used in these two papers is an in-situ polymerization method, and there is a problem that it is difficult to elute the pirimicarb bound to the polymer.
Gao et al. published the article "Molecular imprinted material prepared by novel surface imprinting technique for selective adsorption of pirimicarb" in their paper "Polymer" 2008, 49, 1230-1238 (the preparation of anti-pirimicarb by novel surface polymerization technology has selective adsorption Molecularly imprinted polymers) synthesized anti-pirimicarb molecularly imprinted polymers by surface grafting, but this article only stayed at the evaluation stage of polymer physical characteristics and adsorption properties

Method used

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  • Preparation method of pirimicarb molecularly imprinted polymer
  • Preparation method of pirimicarb molecularly imprinted polymer
  • Preparation method of pirimicarb molecularly imprinted polymer

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Experimental program
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Embodiment 1

[0025] Combine 0.2 mmol of Pirimicarb, 0.8 mmol of TFMAA, and 4 mmol of EDMA, and dissolve them in 20 mL of heptane. After standing at room temperature for 2 hours, add the initiator ACPA-silica gel 0.08 mmol.

[0026] Put the above mixed solution in an ampere bottle and deaerate for 10 minutes with ultrasonic vibration. The degassed system was flushed with nitrogen for 5 minutes. Seal quickly under nitrogen protection. The reaction was kept at 65°C in a water bath for 30 hours. The reaction product is obtained.

[0027] After the reaction, the template molecule was removed by Soxhlet extraction for 12 hours. The extraction reagent is 150ml methanol-water-acetic acid mixture (7:2:1, V / V / V). Finally, wash the remaining acetic acid with methanol to neutrality. The polymer from which the template molecule has been removed is dried at 60°C overnight to obtain a molecularly imprinted polymer of Aphicarb. As determined by experiment,

[0028] The theoretical adsorption capacity of t...

Embodiment 2

[0030] Combine 0.2 mmol of Pirimicarb, 0.8 mmol of TFMAA, and 4 mmol of EDMA, and dissolve them in 20 mL of heptane. After standing at room temperature for 2 hours, add the initiator ACPA-silica gel 0.08 mmol.

[0031] Put the above mixed solution in an ampere bottle and deaerate for 5 minutes with super-sonic vibration. The degassed system was deaerated with nitrogen for 10 minutes. Seal quickly under nitrogen protection. The reaction was kept at 70°C in a water bath for 20 hours. The reaction product is obtained.

[0032] After the reaction, the template molecule was removed by Soxhlet extraction for 12 hours. The extraction reagent is 150ml methanol-water-acetic acid mixture (7:2:1, V / V / V). Finally, wash the remaining acetic acid with methanol to neutrality. The polymer from which the template molecule has been removed is dried at 60°C overnight to obtain a molecularly imprinted polymer of Aphicarb As determined by experiment,

[0033] The molecularly imprinted polymer prep...

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Abstract

The invention discloses a preparation method of a pirimicarb molecularly imprinted polymer in the field of biochemical technology, which comprises the following steps: dissolving a template molecule, i.e. pirimicarb, a functional monomer i.e. trifluoromethyl acrylic acid (TFMAA) and a cross-linking agent, i.e. ethylene glycol dimethacrylate (EDMA) into a pore-forming agent according to the molar ratio of template molecule to functional monomer to cross-linking agent of 1:4:20; adding an initiator; carrying out nitrogen protection and then performing a water bath heat preserving reaction; removing the template molecule through Soxhlet extraction; and drying the polymer at 60 DEG C overnight after the template molecule is removed to obtain the pirimicarb molecularly imprinted polymer. The invention effectively purifies the matrix, efficiently enriches the pirimicarb, and remarkably improves the detection sensitivity and the detection efficiency of the pirimicarb by being combined with the prior detection methods (HPLC and the like).

Description

Technical field [0001] The present invention relates to a preparation method in the field of biochemical technology, in particular to a preparation method of a molecularly imprinted polymer (MIP). Background technique [0002] Aphicarb can control all aphids that are resistant to organophosphate insecticides except cotton aphids. It is often used to control aphids in vegetables. The toxic mechanism of carbamate pesticides is mainly to inhibit cholinesterase activity, so that the hydroxyl group of serine in the active center of the enzyme is carbamylated, thus losing the enzyme's ability to hydrolyze acetylcholine. Due to the over-limit and over-range use of Aphiprocarb, the pressure on the environment and food safety caused by this has become increasingly prominent. Countries around the world have made strict regulations on the use and residues of carbamate pesticides and formulated corresponding standards. Among them, the limit standard for aphiprocarb in pollution-free vegetab...

Claims

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Application Information

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IPC IPC(8): C08F292/00C08F222/14C08F220/04C08J9/26B01J20/285B01J20/30
Inventor 孙向军唐波陈秋宇
Owner SHANGHAI JIAO TONG UNIV