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Chlamydomonas strain and application thereof

A technology of Chlamydomonas and algae strains, which is applied in the fields of application, single-cell algae, edible oil/fat, etc., and can solve the problems of no Chlamydomonas and other problems

Active Publication Date: 2012-03-28
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are a lot of reports on Chlamydomonas gene research, such as patent CN200610026203.3 Chlamydomonas red fluorescent marker protein gene CrmRFP, its synthesis method and the construction method of eukaryotic expression vector, CN200810066705.8 Transgene expressing human tissue kallikrein Construction method of Chlamydomonas reinhardtii, CN 200610018306.5 An exogenous gene expression system of Chlamydomonas reinhardtii and its method for constructing and producing PHB transgenic algae, but there is no report on the production of biodiesel by Chlamydomonas reinhardtii. The present invention provides a Chlamydomonas, which can be further genetically modified

Method used

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  • Chlamydomonas strain and application thereof
  • Chlamydomonas strain and application thereof
  • Chlamydomonas strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening of Chlamydomonas oleaginica strains

[0034] Take the water sample of the small river taken back from the Daqi area after dilution with sterile water. After observing under a 400-fold microscope, the approximate cell concentration is 800-1200 cells / ml. Use a capillary to take about 1ul of the algae liquid and inoculate it in 48 wells In the culture plate with BG11 medium, at 25°C, the light intensity is 50umol / m 2 Cultivate under the condition of .s, when a certain cell concentration is reached, observe with a microscope, select a well with only a single algae strain, and spread the plate to obtain a single algae strain.

[0035] Put pure algal strains in BG11 medium, 25°C, light intensity 50umol / m 2 Cultivate under .s, when the concentration of algae reaches 3g / l, turn on the lamp to make the light intensity reach 250umol / m 2 .s, for lipid induction;

[0036] Carry out Nile Red staining with single algae strain, the oil in the algae is dyed col...

Embodiment 2

[0037] Example 2 Determine the classification of algal species on the evolutionary tree.

[0038] The identification of algae strains adopts the combination of shape identification and molecular identification.

[0039] shape identification (see figure 2 ): The algae strain DQA-01 isolated above was observed under a 1000-fold microscope, and it was found that the algae body was single-celled, spherical or oval, with two equal-length flagella at the front end, capable of swimming. There are two telescopic vesicles at the base of the flagella; and one red eye spot at the proximal end of the cell. Large cup-shaped chromophore with a starch nucleus. Asexual reproduction produces zoospores; sexual reproduction is isogamous, heterogamous, and oviparous. Under unfavorable living conditions, the cells stop swimming and undergo multiple divisions, forming a temporary group called "indeterminate group". Retrieval of "Freshwater Algae in China--System, Classification and Ecology",...

Embodiment 3

[0053] Example 3 Chlamydomonas lipid accumulation

[0054] The algal species in the logarithmic growth phase are inoculated in the prepared medium, and the growth culture and oil induction culture are carried out by adjusting the light intensity.

[0055] Media and general methods for Chlamydomonas lipid accumulation are as follows:

[0056] A. Culture medium preparation:

[0057] Prepare the culture medium according to the BG11 formula in the following table

[0058] NaNO 3 0.8--1.5g / l

[0059] K 2 HPO 4 .3H 2 O 0.02-0.08g / l

[0060] MgSO 4 .7H 2 O 0.075g / l

[0061] CaCl 2 .2H 2 O 0.036g / l

[0062] citric acid 0.006g / l

[0063] Ferric ammonium citrate 0.006g / l

[0064] EDTA (dinatrium-salt) 0.001g / l

[0065] Na 2 CO 3 0.02g / l

[0066] A5+Co solution * 1ml

[0067] Deionized water 919ml

[0068] * Composition of A5+Co solution

[0069] Add to 1000ml deionized water

[0070] h 3 BO 3 ...

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Abstract

The invention provides a chlamydomonas strain. Through authentication, the chlamydomonas strain is a novel strain, and the collection number of the chlamydomonas strain is CGMCC No.3577. The chlamydomonas strain has higher grease content and can be applied to preparing biodiesel, feed or edible oil.

Description

technical field [0001] The invention belongs to the field of bioenergy, and in particular relates to a Chlamydomonas and its application for preparing biodiesel, more specifically, the present invention relates to a new strain of Chlamydomonas, which has good oil content and can be used for preparing biodiesel, Feed or edible oil application. Background technique [0002] With the increasingly severe environmental deterioration, controlling vehicle exhaust emissions and the greenhouse effect and protecting the natural environment on which human beings live have become urgent problems for human beings to solve. At the same time, the global energy demand continues to expand, and the search for renewable and clean energy that can replace petroleum in the energy structure is currently a hot spot of widespread concern. [0003] Microalgae are a class of low-level plants that grow in water with a wide variety and are extremely widely distributed. It is a cell factory driven by su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/13C12P7/64A23K1/14C12R1/89C12P7/649
CPCC10G2300/1014A23K1/14C11C3/003Y02E50/13C10L1/026C12P7/649C12P7/6463A23D9/00C12R1/89Y02P30/20A23K10/30Y02E50/10C12R2001/89C12N1/125
Inventor 吴洪黄龙耀李青吴义诚
Owner ENN SCI & TECH DEV
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