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Method for producing ethanol by simultaneous high-efficiency saccharification half enzymolysis and fermentation of straw lignocellulose

A lignocellulose, synchronous fermentation technology, applied in microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of low yield of cellulose enzymatic hydrolysis, long cellulose enzymatic hydrolysis time, and low sugar concentration, etc. question

Active Publication Date: 2013-07-17
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The initial production of ethanol by enzymatic hydrolysis and fermentation of cellulose is carried out in steps of enzymatic hydrolysis and fermentation. When the natural lignocellulosic raw material is directly subjected to enzymatic hydrolysis after pretreatment, due to the characteristics of the cellulose structure, the enzymatic hydrolysis of cellulose takes a long time and complete enzymatic hydrolysis It takes 72 hours to decompose, and at the same time, due to the feedback inhibition of the enzyme by the cellobiose and glucose produced during enzymatic hydrolysis, the yield of cellulose enzymatically hydrolyzed sugar is low, and the sugar concentration is low (within about 12%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Add 1.8kg of corn stalks with a solid-to-liquid ratio of 1:5 (weight to volume ratio), add sodium hydroxide solution, and cook at 105°C for 1.5 hours with a lye concentration of 1.8%. Centrifuge after the end of the reaction, and wash the filter residue with hot water to obtain 3.76kg of filter residue (moisture content 71%, cellulose content 62.2% in dry basis, hemicellulose content 25.3%, lignin content 8.2%, others are ash) .

[0019] Then add in the reactor with agitation, add water to adjust dry substance concentration to 18%, adjust pH to 4.8, temperature 50 ℃, add cellulase (20FPIU / gram cellulose) and xylanase (30FPIU / gram cellulose), Stirring speed 200rpm / min, enzymolysis 36 hours, the total sugar concentration of DNS method measurement enzymolysis solution, the glucose and xylose concentration in high performance liquid chromatography survey enzymolysis solution (total sugar concentration 13.54%, glucose concentration 8.10%, Xylose concentration 3.92%).

[00...

Embodiment 2

[0022] 7.2 kg of corn stalks, with a solid-to-liquid ratio of 1:4.5 (weight-to-volume ratio), was added with sodium hydroxide, the concentration of lye was 2.0%, and steamed at 120° C. for 0.5 hour. Centrifuge after the reaction and wash the filter residue with hot water to obtain 14.0 kg of filter residue (70.78% moisture, 61.59% cellulose on a dry basis, 24.90% hemicellulose, 7.19% lignin, and 10.74% ash).

[0023] Add in a stirred reactor, add water to adjust dry matter concentration to 16%, adjust pH to 4.9, temperature 50°C, add cellulase (25FPIU / gram cellulose) and xylanase (20FPIU / gram cellulose), enzyme After 16 hours of solution, the system was cooled to 33°C, 400mL of nitrogen source (2% concentration urea) and 3.5L of Saccharomyces cerevisiae seed liquid were added, the concentration was 300 million / ml, and the fermentation was started. Sampling was performed for 60 hours, and the ethanol concentration was determined to be 5.6 by gas chromatography. (v / v), the total...

Embodiment 3

[0025] 6.4kg corn cobs, solid-liquid ratio 1:4 (weight to volume ratio), add sodium hydroxide, lye concentration 1.6%, cook at 110°C for 1 hour. Centrifuge after the reaction, wash the filter residue with hot water to obtain 13.55kg of filter residue (moisture content 76.16%, cellulose 62.25% in dry basis, hemicellulose 24.17%, lignin 8.68%, others are ash).

[0026] Add in a stirred reactor, add water to adjust the dry matter concentration to 20%, adjust the pH to 4.9, and add cellulase (20FPIU / gram cellulose) and xylanase (20FPIU / gram cellulose), enzyme After 24 hours of solution, the system was cooled to 33°C, and 400 mL of nitrogen source (2% concentration urea) and 4.0 L of S. cerevisiae seed solution were added at a concentration of 300 million cells / ml to start fermentation. Blowing after 72 hours of fermentation, the ethanol concentration measured by gas chromatography is 5.92 (v / v), and the total wine production is 1235g, and the corncob (dry weight) per ton productio...

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Abstract

The invention provides a method for producing ethanol by simultaneous high-efficiency saccharification half enzymolysis and fermentation of straw lignocellulose. A pretreated straw lignocellulose raw material is subjected to pre-enzymolysis, and then is added with saccharomyces cerevisiae for simultaneous enzymolysis and fermentation. In the method, the pretreated straw lignocellulose raw material is subjected to the pre-enzymolysis process, most of the cellulose in the raw material (about 80 percent of the total cellulose) is transformed into micromolecular sugar during the process, the raw material is added with a certain amount of yeast for simultaneous enzymolysis and fermentation, the rest cellulose raw material is continuously subjected to the enzymolysis, and glucose in the enzymolysis process is timely transformed into the alcohol simultaneously, so that the feedback inhibition in the enzymolysis process is removed, and the yield of the cellulose-transformed alcohol is improved.

Description

technical field [0001] The invention relates to a method for producing ethanol through high-efficiency saccharification and semi-enzymatic hydrolysis of straw lignocellulose and synchronous fermentation, which belongs to the technical field of biomass chemical industry. Background technique [0002] The main components of lignocellulosic resources are cellulose, hemicellulose and lignin. Among them, cellulose and hemicellulose are the source of fermentable sugar, and the content accounts for 66-75% of the wood fiber (the dry weight of the cellulosic raw material). Straw lignocellulose exists in a large amount in nature and can be continuously regenerated, but its utilization is quite low, and only a very small part is used as papermaking raw material, feed and chemical preparation. The production of ethanol from lignocellulose is the most widely used topic of energy utilization of this kind of raw material. [0003] The structure of lignocellulosic raw materials is very co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 黄之文吴真汪坤冯杰
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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