Preparation method of egg white protein assistant antihypertensive peptides

A blood pressure-lowering peptide, egg white protein technology, applied in the direction of hydrolyzed protein components, peptide/protein components, medical preparations containing active ingredients, etc., can solve the problems of high reaction termination temperature, heavy workload, long processing cycle, etc., to achieve The effect of low preparation temperature, simple method and good oxidation resistance

Inactive Publication Date: 2010-12-08
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
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Problems solved by technology

[0003] The present invention aims to solve the problem of large desalination treatment workload, toxic eluent acetonitrile and the processing period of the chymotrypsin hydrolysis separation method in the preparation process of the existing pepsin hydrolysis separation method for separating angiotensin converting enzyme inhibitory peptide from ovalbumin Long, high reaction termination temperature, and provide egg white protein source auxiliary blood pressure lowering peptide preparation method

Method used

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  • Preparation method of egg white protein assistant antihypertensive peptides
  • Preparation method of egg white protein assistant antihypertensive peptides

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specific Embodiment approach 1

[0009] Specific embodiment one: the preparation method of the egg white protein source assisted blood pressure lowering peptide of the present embodiment is carried out according to the following steps: 1. Proteolysis: according to the mass ratio of egg white and protease is 100: 1~3, weigh egg white and protease, the egg white Prepare an aqueous protein solution with a concentration of 3% to 10% (mass) and place it in a reactor, heat up to 30°C to 50°C, and adjust the pH value of the aqueous protein solution to 5 to 8, then add protease, at a temperature of 30 Under the condition of ℃~50℃, stir for 2h~5h to obtain the protein hydrolyzate; 2. Desalination treatment: pass the protein hydrolyzate obtained in step 1 through the strong acidic cation at a flow rate of 7 times to 9 times the column volume per hour. exchange resin column, and then pass through a strong basic anion exchange resin column to collect the effluent; 3. Fractional purification treatment: the effluent obtaine...

specific Embodiment approach 2

[0011] Specific embodiment two: this embodiment is different from specific embodiment one: the protease described in step one is pepsin, trypsin, papain, bromelain, neutral protease, alkaline serine protease (Alcalase) and mold One or a combination of several proteases. Others are the same as in the first embodiment.

[0012] In this embodiment, when protease is a composition, various proteases are combined in arbitrary ratios.

specific Embodiment approach 3

[0013] Embodiment 3: This embodiment is different from Embodiment 1 or 2 in that: in step 1, the mass ratio of egg white to protease is 100:1.2-2.8. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a preparation method of egg white protein source assistant antihypertensive peptides, and relates to a preparation method of antihypertensive peptides. The method solves the problems of heavy workload of desalinization processing of the traditional pepsin hydrolysis separation method in the preparation process, venomous eluent acetonitrile, long processing period and high reaction end temperature of a chymotrypsin hydrolysis separation method. The method comprises the following steps of: stirring egg white with proteinase for hydrolyzation under the conditions that the pH value is 5 to 8 and the temperature is 30 to 50 DEG C, then sequentially desalting hydrolyzate through strong acid cation exchange resin columns and strong basicity anion exchange resin columns, and filtering effluent liquid through an ultrafiltration membrane and purifying the effluent liquid through gel filtration pre-installed columns Superdex Peptide 10-300GL to obtain the egg white protein assistant antihypertensive peptides. Molecular weight distribution of the antihypertensive peptides is from 200Da to 2282Da, the antihypertensive peptides have nutrition and blood pressure lowering effect, the preparation conditions are mild and simple, the workload of desalination is small, and the antihypertensive peptides can be used as health-care food for patients with elevated blood pressure.

Description

technical field [0001] The present invention relates to the preparation method of hypotensive peptide. Background technique [0002] Hypotensive peptides are angiotensin-converting enzyme inhibitory peptides. Angiotensin-converting enzyme (ACE) inhibitors refer to a class of polypeptide substances that can inhibit ACE activity. Although the types and quantities of amino acids that make up these polypeptides are different, However, these peptides all have the potential characteristic function of inhibiting ACE activity and assisting the treatment of hypertension, and are effective drugs for dealing with hypertension today. Angiotensin-converting enzyme inhibitory peptides isolated from natural food raw materials, such as casein, whey protein, fish protein, soybean protein, rice protein, corn protein, peanut protein, egg protein, etc., are safe, No side effects and widely people's attention. Existing methods for isolating angiotensin-converting enzyme inhibitory peptides fro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06A61K38/01A61K38/17A61P9/12
Inventor 迟玉杰王喜波夏宁张华江
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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