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Thick oil-degrading bacteria and application thereof

A technology for degrading bacteria and heavy oil, applied in the field of biological treatment of environmental pollutants, to achieve the effect of high-efficiency heavy oil removal rate and good environmental adaptability

Inactive Publication Date: 2011-12-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention mainly aims at the problems faced by the above-mentioned existing heavy oil pollution remediation technology, and provides a high-efficiency heavy oil degrading bacterium, and its application mainly in wastewater biological treatment and contaminated soil environment remediation

Method used

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  • Thick oil-degrading bacteria and application thereof
  • Thick oil-degrading bacteria and application thereof
  • Thick oil-degrading bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Screening and Isolation of Strain CCTCC M 2010172 and Its Heavy Oil Degradation Performance

[0024] The mud-water mixture polluted by petroleum hydrocarbons in Guangzhou Petrochemical General Plant was collected on site, and heavy oil-degrading bacteria were acclimated using an inorganic salt liquid culture medium system with heavy oil as the only carbon source. Inorganic salt liquid medium: KH 2 PO 4 0.5g, K 2 HPO 4 ·3H 2 O 1g, NaCl 2g, MgSO 4 Aqueous solution (22.5g / L) 3.0mL, CaCl 2 Aqueous solution (36.4g / L) 1.0ml, FeCl 3 Aqueous solution (0.25g / L) 1.0ml, trace element solution 1.0ml, distilled water 1L. Among them, the trace element solution composition: MnSO 4 ·H 2 O 39.9mg / L, ZnSO 4 ·H 2 O42.8mg / L, (NH 4 ) 6 Mo 7 o 24 4H 2 O 34.7mg / L. The initial concentration of heavy oil is 1g / L. After several generations of repeated acclimatization, streak culture in solid medium (NR) to obtain multiple strains of pure bacteria, spread the pure bacteria on a ...

Embodiment 2

[0029] Effect of Temperature on Degradation of Heavy Oil

[0030] The thick oil inorganic salt liquid medium inoculated with GS02 was placed in a constant temperature shaker at 25, 28, 30, 35 and 40 °C for 6 days, and then the effect of temperature on the degradation of heavy oil was determined.

[0031] Table 1

[0032] Temperature (℃) Degradation rate (%)

[0033] 25 34.5

[0034] 28 38.7

[0035] 30 45.7

[0036] 35 39.5

[0037] 40 27.4

[0038] It can be seen from Table 1 that the degrading bacteria GS02 has a certain removal effect on heavy oil in the range of 25-35 °C, and the degradation effect is the best at 30 °C, which is 45.7%. In the wide temperature range of 25-35 °C, the degradation effect does not change significantly. When the temperature reaches 40°C, the degradation rate decreases, which is because the protein in the bacterial cell is easily denatured and the activity of the enzyme is inhibited in a high temperature environment. Since the temperature i...

Embodiment 3

[0041] Effect of pH Value on Degradation of Heavy Oil

[0042] The pH value of the inorganic salt liquid medium was adjusted to 6.5, 7.0, 7.2, 7.5 and 8.0 respectively, and the initial concentration of heavy oil was 1g / L, and cultured for 6 days to determine the effect of pH value on the degradation of heavy oil.

[0043] Table 2

[0044] pH degradation rate (%)

[0045] 6.5 31.5

[0046] 7.0 45.7

[0047] 7.2 47.6

[0048] 7.5 38.3

[0049] 8.0 34.9

[0050] As can be seen in Table 2, when the pH value is 7.2, the degrading bacteria GS02 has the strongest degradation effect on heavy oil, and the degradation rate can reach 47.6% in 6 days; when the pH value is 6.5, the degradation rate is lower, 31.5%. The pH value mainly affects the activity of bacterial enzymes, the permeability and stability of the plasma membrane, thus affecting the degradation rate of petroleum hydrocarbons. The degrading bacteria GS02 has a high degradation effect on heavy oil in the neutral or alk...

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Abstract

The invention discloses thick oil-degrading bacteria and application thereof. The bacterial strain is Acinetobacter sp. GS02 collected by China Center for Type Culture Collection on July 8, 2010, with the collection number of CCTCC M 2010172. The bacterial colony of the bacteria is round, has smooth protrusions and neat edges, is off-white and has viscosity. The bacteria are sporeless coccobacillus when observed under an electron microscope. The bacteria have the biological characteristics of oxidase negative, catalase positive, gram stain negative, no fermentation and obligate aerobe. The bacterial strain has good effect of removing alkane components, nonbiodegradable pristine and phytane in thick oil, and has high-efficiency thick oil removal rate and good adaptive capacity to environment compared with other bacterial strains. Based on the characteristics, the bacteria can be well applied to the restoration of thick oil-polluted water and soil.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, and in particular relates to a bacterium strain for degrading heavy oil and its application in biological treatment of wastewater and environmental restoration of polluted soil. Background technique [0002] Crude oil is mainly composed of alkanes, aromatics, colloids and asphaltenes. Heavy oil is a kind of crude oil, and its main characteristics are high colloidal asphaltenes, high wax content and high viscosity. With the gradual reduction of the production of medium crude oil and light crude oil, the problem of water and soil pollution caused by heavy oil has become more and more serious, which has attracted people's attention day by day. Due to the characteristics of high colloidal asphaltene and high viscosity of heavy oil, compared with petroleum, it is more difficult to remove in the environment, and the existence time in the environment will be lon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34A62D101/20B09C1/10C12R1/01C12N1/20A62D3/02C02F103/10
Inventor 党志贾群超卢桂宁易筱筠郭楚玲杨琛
Owner SOUTH CHINA UNIV OF TECH
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