Method for detecting chemical components of ether extract of bitter gourd leaves

A detection method and a technology for chemical components, applied in the field of comprehensive development of plant resources

Inactive Publication Date: 2013-01-23
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no relevant literature reports on the chemical components in bitter gourd leaves that have insecticidal activity against larvae.

Method used

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  • Method for detecting chemical components of ether extract of bitter gourd leaves
  • Method for detecting chemical components of ether extract of bitter gourd leaves
  • Method for detecting chemical components of ether extract of bitter gourd leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Bitter Melon Leaf Ether Extract:

[0038] Weigh 2 g of fresh bitter melon leaves that have passed through, grind them into powder, add 200 mL of ether, and repeatedly extract with a Soxhlet extractor until it is colorless, remove the solvent with a rotary evaporator, weigh the obtained extract, and redissolve the extract in 100mL of diethyl ether; then take 10mL of diethyl ether extract, add 1ml of diethyl ether-n-hexane mixture with a volume ratio of 2:1, 5ml of methanol, 1ml of 0.8mol / L potassium hydroxide methanol solution, shake well, let stand for 5min, add 5ml of water, 300rPm Centrifuge for methyl esterification, take the supernatant for GC-MS analysis.

Embodiment 2

[0040] Adopt GC-MS to analyze, gas chromatography conditions:

[0041] The initial temperature of the column is 60°C for 2 minutes, raised to 150°C at 5°C / min and held for 3 minutes, then raised to 220°C at 10°C / min and held for 3 minutes, the temperature of the injection port: 250°C; High-purity helium was used as the carrier gas, the flow rate was 1.0ml / min, the split ratio was 60:1, and the injection volume was 1.0 μL.

[0042] Mass spectrometry conditions: mass spectrometry ionization mode is EI, electron energy of electron bombardment source is 70eV, ion source temperature is 250°C; electron multiplier voltage is 1.0×10 5 ; Solvent delay time: 3min; Scanning range m / z: 35-1000aum.

[0043] According to the above-mentioned GC-MS analysis conditions, the bitter melon leaf alcohol-soluble components are tested on the machine, and the total ion current chromatogram of the bitter melon leaf ether extract (see figure 1). The identification of each peak was mainly through the...

Embodiment 3

[0045] (1) Bitter melon leaf ether extract:

[0046] Weigh 2g of fresh balsam pear leaves and grind them into powder, add 200mL of diethyl ether, repeatedly extract with a Soxhlet extractor until colorless, remove the solvent, weigh, and re-dissolve the extract in 100mL of diethyl ether; then take 10mL of ether extract, Add 1ml of diethyl ether-n-hexane mixture with a volume ratio of 2:1, 5ml of methanol, and 1ml of 0.8mol / L potassium hydroxide methanol solution, shake well, let stand for 5min, add 5ml of water, centrifuge at 300rpm, carry out methyl esterification treatment, and set aside;

[0047] (2) Analyze by GC-MS, gas chromatographic conditions: the initial column temperature is 60°C for 2 minutes, rise to 150°C at 5°C / min and hold for 3 minutes, then rise to 220°C at 10°C / min, hold for 3 minutes, inject the sample Inlet temperature: 250°C, mass spectrometry connection line temperature: 250°C, high-purity helium as carrier gas, flow rate 1.0ml / min, split ratio: 60:1, in...

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Abstract

The invention discloses a method for detecting the chemical components of ether extract of bitter gourd leaves, in particular a method for detecting the chemical components of bitter gourd leaves by a GC-MS technique and the result of the detection. In the invention, extract solution is extracted from the bitter gourd leaves in an organic solvent, the extract solution is subjected to methyl esterification, the product of the methyl esterification is subjected to separation by capillary column chromatography, the mass spectrum of the obtained sample is compared with spectra in a standard spectrum library, and relative content is calculated by a peak area normalization method. 45 chemical components are identified from the ether extract of the bitter gourd leaves, and account for 54.2 percent of the total appearance area. Results show that the main ingredients of the ether extract of the bitter gourd leaves include nonadecane, tetradecane, n-hexadecanoic acid and the like. The method lays a foundation and provides a real evidence for the development and utilization of the bitter gourd leaves as a plant resource and.

Description

technical field [0001] The invention belongs to the technical field of comprehensive development of plant resources, and relates to a method for detecting chemical components of plants and vegetables using modern analytical instruments, more specifically, a method for detecting chemical components in different extracts of bitter melon leaves. Background technique [0002] Bitter gourd (Momordica Charantia L), also known as Jin litchi, skinny grape, skinny melon, cold melon, etc., is a vine herb of Cucurbitaceae Momordica balsam pear. Treating stomach pain, dysentery, treating sores and swelling, repelling insects, etc., through field investigations and experiments, it has been confirmed that bitter melon leaves and their extracts also have strong insect resistance. K.Prabakar et al. The methanol extracts from the leaves of these five cucurbit plants had the insecticidal activity against Culex quinquefasciatus larvae. 50 The value is 465.85ppm, which is lower than the other ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/06
Inventor 陈宏徐秋曼郭红梅李翔单少杰吴志杰雷盯函
Owner TIANJIN NORMAL UNIVERSITY
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