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Method for preparing nanoemulsion from high-efficient active sites of fructus forsythiae essential oil

A technology of active parts and volatile oil, applied in the field of nanoemulsion, can solve the problems of high oral irritation, similar structure, no research on volatile oil of Forsythia suspensa, etc., to achieve good therapeutic effect and improve the effect of pharmacological action

Inactive Publication Date: 2012-10-31
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of volatile oil is complex and similar in structure; it has a wide range of pharmacological effects such as antibacterial, antiviral and anti-inflammatory effects, but it has certain irritation, especially the oral irritation is greater
For a long time, there has been no in-depth research on the volatile oil of forsythia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Silica gel column chromatography separates different parts

[0020] Pack the column by wet method, add 200mL of petroleum ether to 70g of silica gel, remove air bubbles, load the column, load 1g of forsythia volatile oil, add petroleum ether: ethyl acetate = 15:1; 13:1; 11: 1; 9: 1; 7: 1; 5: 1; 3: 1; 1: 1; 1: 3; 1: 5 elution, each eluent 200mL, divided into separate tubes to recover 10mL per tube, spotting, with sulfuric acid Ethanol for color development. It is sufficient to recover parts with approximately the same polarity.

Embodiment 2

[0021] Embodiment 2: Different parts are separated by low-pressure vacuum fractionation

[0022] 2.1. Wash the tower with absolute ethanol, turn on the condensed water, wash the tower for 3 hours under the condition of total reflux at normal pressure, and then use a vacuum pump to dry the ethanol vapor in the tower.

[0023] 2.2. Measure an appropriate amount of forsythia volatile oil, add it and zeolite into the cleaned tower kettle; seal the rectification tower and ensure its airtightness; turn on the vacuum pump to control the pressure at the top of the tower to -0.07MPa, and start heating until the volatile oil reaches Slightly boiling state (150℃~154℃).

[0024] 2.3. Adjust the rectification tower insulation device to increase the temperature gradient, that is, 25°C~30°C→58°C~62°C→64°C~72°C→85°C~116°C→120°C~130°C; The rectification parts are B1, B2, B3, B4, B5, B6. Note: The equipment parameters of the rectification tower are: the inner diameter is The height of the t...

Embodiment 3

[0025] Embodiment 3: Antibacterial experiment of different active sites

[0026] (1) Activation of strains: test strains (standard strains) were inoculated on solid agar medium, activated at 37° C. for 24 hours, and Staphylococcus aureus was activated for 48 hours.

[0027] (2) Preparation of bacterial suspension: the activated test strains were picked up with an inoculation loop and placed in normal saline to prepare a bacterial suspension with a turbidity of about 0.5 Mcf for future use.

[0028] (3) Preparation of each active part solution: each active part of each subject was dissolved in 1.0% HAc solution, and the concentration of the volatile oil of Forsythia was set at 2%, 1.5%, 1.0%, 0.5%, 0.25%.

[0029] (4) Antibacterial test method (agar diffusion disc method)

[0030] The disc diffusion method is to stick a filter paper disc containing quantitative antibiotics on the surface of agar inoculated with test bacteria, the drug in the disc diffuses in the agar, and the ...

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Abstract

The invention relates to a method for separating high-efficient anti-pathogenic microorganism active sites from fructus forsythiae essential oil and preparing the high-efficient active sites into nanoemulsion. The method relates to the separation of high-efficient active sites of fructus forsythiae essential oil and the preparation of nanoemulsion, and concretely comprises the following steps: separating through a functional graphene oxide or a low-pressure decompression fractionation method to obtain different active sites of fructus forsythiae essential oil; screening the active sites through antibiotic or anti-virus tests to obtain high-efficient active sites; weighting 1.0-2.0% of high-efficient active sites of fructus forsythiae essential oil, 2-3% of glycerol and 10-30% of Tween-80,mixing evenly, then slowly adding the mixture to balance water for injection, stirring evenly, standing at 1-4 DEG C for 2-5 days; and using millipore filters with the aperture of 0.45 mu m and 0.22 mu m to sequentially filter to obtain the nanoemulsion prepared from the high-efficient active sites of fructus forsythiae essential oil. Through the method, the pharmacological effects of essential oil is improved, the stimulation of fructus forsythiae essential oil is reduced, the activity of the essential oil is enhanced, and better therapeutic effect can be obtained through oral administration.

Description

technical field [0001] The invention relates to a method for isolating high-efficiency anti-pathogenic microorganism active parts from forsythia volatile oil and preparing them into nanoemulsions, belonging to the technical field of medicine. Background technique [0002] With the globalization of the economy, the globalization of information logistics, and the deterioration of the ecological environment, the globalization of plague, that is, infectious diseases, has also emerged. The generation and spread of the plague is mainly due to human infection with pathogenic microorganisms including bacteria and viruses. Therefore, the treatment of infectious diseases is mainly to use antibiotics and antiviral drugs. At present, the antibiotics and antiviral drugs on the market are all chemically synthesized or semi-synthetic drugs with clear structure and single composition. They have indeed played a significant role in the prevention and treatment of infectious diseases when they...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/634A61K9/107A61P31/04A61P31/12
Inventor 郝保华李伟泽
Owner NORTHWEST UNIV