Fast propagation method of beautybush

Abstract

The invention discloses a fast propagation method of beautybush, which comprises the steps of: performing promoting induction of auxiliary buds in case of taking stem with buds of beautybush as explant, bringing ramets of the inducible auxiliary bud into enrichment culture to obtain auxiliary seedlings, and then bringing ramets of the auxiliary seedlings into rooting culture to obtain regenerative plants. The method according to the invention breaks out of the seasonal limitation to beautybush propagation, has convenient acquisition of raw materials, namely in vitro organs of beautybush can be acquired at any time, can perform fast induction of the auxiliary seedlings in large quantities to obtain the regenerative plants so as to greatly shorten the propagation period, and further has theadvantages of the survival rate of the regenerative plants as high as 100%, small variation and high hereditary stability.

Description

technical field [0001] The invention belongs to the technical field of plant isolated tissue culture methods, in particular to a method for rapid propagation of plant fruit. Background technique [0002] Hedgehog (Kolkwitzia amabilis Graebn) is a deciduous shrub of the genus Kolkwitzia in the family Caprifliaceae. It is a single-species plant unique to China discovered and named by German botanist Richard Kolkwitz in 1901. It is found in Shanxi, Shaanxi, and Gansu in my country. There are wild distributions in China, Henan, Hubei, Anhui and other places. The National Environmental Protection Agency's 1987 "List of Endangered and Protected Plants in China" designated Hedgehog as a third-level rare and endangered plant. Hedgehog is in an isolated position in Lonicaceae, and it is an ancient remnant single-species plant in the flora from the Qinling Mountains to the Dabie Mountains in China. It has high scientific value for the study of flora, paleogeography and Lonicera phyloge...

AI Technical Summary

Problems solved by technology

The community of Hedgehog is xeric shrub or open forest zone, the community composition is simple, the species diversity is low, the community of Hedgehog is in the primary stage of succession, and the stability is poor ("The appearance and species of Hedgehog plant community Research on diversity"-"Hubei Forestry Science and Technology" 2005.4)

At present, the breeding method of Hedgehog is still mainly by sowing, cutting, branching and layering ("Reproduction and Cultivation Technology of Rare Garden Plant Hedgehog and Its Development and Utilization" - "Modern Agricultural Science and Technology" 2007.19; "Phenology of Rare Flower Plant Hedgehog Scientific Observation and Cultivation Technology"-"Jiangsu Agricultural Science" 2003.5), due to the above reasons, the limitations of sowing and breeding of hedgehogs, seasonal limitations of cuttings, ramets and layering, long cycle and low reproduction coefficient have always hindered rapid mass reproduction

There are few studies on the tissue culture and rapid propagation methods of Hedgehog, and Tiemei et al. Hypocotyls were used as test materials to conduct callus and organ differentiation studies, and it was proposed that the combination of BA (1-3) mg / L and NAA (0.1-0.2) mg / L can induce the differentiation of callus buds from hedgehog hypocotyls , 0.2mg / L NAA or IBA can induce rootless seedlings to take root, but no effective in vitro culture and rapid propagation system has been established; at the same time, hypocotyls are used as explants, on the basis of germination of healthy seeds to form sterile seedlings It can only be carried out. The various characteristics of hedgehog seeds have been described above. To germinate them, not only special methods for removing dormancy are required, but the germination rate is still not ideal, and it is difficult to obtain hypocotyl explants; use other tissues as explants The process of callus induction, differentiation, proliferation and rooting is also relatively cumbersome and takes a long period, making it difficult to meet the requirements of rapid multiplication of hedgehogs.

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