Preparation method of natural bone repairing material with pre-plasticity

A repair material and natural bone technology, applied in the field of preparation of natural bone repair materials, can solve the problems of no significant improvement in bone reconstruction speed and unfavorable bone tissue reconstruction

Inactive Publication Date: 2011-01-26
SHAANXI RUISHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this type of material does not change the composition of calcium phosphate ceramics, but also introduces bone cement. Its degradation will produce an acidic microenvironment, produce non-bacterial i

Method used

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  • Preparation method of natural bone repairing material with pre-plasticity
  • Preparation method of natural bone repairing material with pre-plasticity
  • Preparation method of natural bone repairing material with pre-plasticity

Examples

Experimental program
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Effect test

Embodiment 1

[0024] 1. Removal of organic matter: take bovine femoral epiphyseal cancellous bone, remove the soft tissue on the surface, machine it into broken bone pieces with a particle size of 1-5mm, stir and wash them with pure water for 30 minutes; use ether solution with 2 times the volume of the bone pieces Shake and wash for 20 minutes, then put the bone fragments into 0.5g / L trypsin solution for digestion at room temperature for 70 hours, stir and wash with pure water for 10 minutes, then place them in 1M urea solution for 6 hours, then put them in 1M NaOH solution Medium-ultrasonic cleaning for 2 hours, replace the solution every half an hour, stir and clean with pure water until neutral, and dry in a 37°C oven for 12 hours.

[0025] 2. Calcination and preparation of bone particles: Put the dried bone fragments into a crucible and calcinate in a muffle furnace; heat up to 800°C and then calcine at a constant temperature for 3 hours, then lower the temperature in the furnace to roo...

Embodiment 2

[0030]1. Removal of organic matter: take bovine femoral epiphyseal cancellous bone, remove the surface soft tissue, machine it into broken bone pieces with a particle size of 1-3 mm, wash with pure water for 30 minutes; shake and wash with ether solution twice the volume of the bone pieces After 20 minutes, the bone fragments were digested in 1g / L trypsin solution at room temperature for 46 hours, washed with pure water for 10 minutes, stirred and washed in 1M urea solution for 6 hours, and then ultrasonically cleaned in 1M NaOH solution for 3 hours. hour, change the solution every half hour, stir and wash with pure water until neutral, and dry in a 37°C drying oven for 12 hours.

[0031] 2. Calcination and preparation of bone particles: Put the dried bone fragments into a crucible and calcinate in a muffle furnace; heat up to 600°C and then calcinate at a constant temperature for 5 hours, then lower the temperature in the furnace to room temperature naturally, take out the cal...

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Abstract

The invention relates to a preparation method of a natural bone repairing material with pre-plasticity. The natural bone repairing material is prepared by the steps of uniformly mixing forged bone particles and a bone collagen solution and freeze-drying at low temperature; protein and a growth factor for promoting bone formation are added into the bone collagen solution. The bone repairing material prepared by the method comprises natural inorganic components, the collagen component and the natural bone component are tightly combined, so that the stability of the material in a transplantation area is ensured and new bone can be rapidly formed. The prepared material can continuously release the active factor for bone formation for a long time, has certain elasticity and tenacity, can be cut and formed freely as required, thereby being convenient for clinic operations. Animal experiments verify that the bone repairing speed of the material is obviously higher than that of other artificial bone repairing materials. The material has simple preparation method and wide and cheap raw material resources, and the material is applicable to large-scale industrial production, can reduce medical cost and can relieve the economical burden of patients.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering biomedical materials, and in particular relates to a preparation method of a preplastic natural bone repair material. Background technique [0002] Trauma, bone tumor and other diseases will cause different degrees of bone defects in various parts, and bone transplantation is one of the main methods to treat bone defects, and autologous bone transplantation usually has the best effect. However, because the source of autogenous bone is limited, and it will increase the patient's pain and surgical complications, some artificial bone graft materials are used in clinical treatment. For example, polymers, bioceramics, calcium phosphate bone cement, and metal materials, etc., and these materials have different shortcomings, which limit their clinical application. [0003] Due to the rapid degradation rate of polymers in the human body, they cannot adapt to the bone reconstruction rate, and w...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/54
Inventor 王爱军杨鹭
Owner SHAANXI RUISHENG BIOTECH
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