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High-efficiency denitrification mixed bacterial agent and application thereof

A mixed bacterial agent and denitrification technology, applied in the directions of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of residual and secondary pollution in water bodies, reduce chlorine consumption, improve efficiency, and improve nitrification. The effect of efficiency

Active Publication Date: 2011-01-26
ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0016] 3) After using chemical products, there will be residues in the water body, which may cause secondary pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The cultivation process of the denitrification bacteria of the present invention:

[0039] The medium ratio is: 2% glucose (w / v), 1.5% beef extract (w / v), 1.5% peptone (w / v), 0.058% magnesium sulfate (w / v), 0.025% manganese sulfate (w / v), 0.22% sodium acetate (w / v), 0.2% dipotassium hydrogen phosphate (w / v), and the solvent is water. The above percentages are percentages by mass and volume, and a component concentration of 1% means that 100 mL of culture medium contains 1 g of the component.

[0040] Fermentation conditions: the initial pH value is 5.8, and CCTCC No: M 2010001 and CCTCC No: M 2010002 are respectively inoculated, maintaining 2% glucose (w / v) and 0.22% sodium succinate (w / v) during the fermentation process. At 30℃, the stirring speed is 400r / min, the aeration rate is 1vvm (7L tank), and the culture is 22h, the number of bacteria in the fermentation broth can reach 1.17×10 10 cfu / mL.

[0041] Sludge domestication process:

[0042] After the microbial fermentati...

Embodiment 2

[0044] For the domestic sewage of Chongzhao Village Ecological Demonstration Village in Huzhou City, the raw water quality is BOD5 (Biology Oxygen Demmand) 250mg / L, COD (chemical oxygen demand) 400mg / L, and total nitrogen 30mg / L. 1000ml of 10mg / L ammonia nitrogen was taken and placed in a glass container. The sludge from the sewage treatment station of the village and the sludge after acclimation according to the method of Example 1 were used for aerobic treatment, and the aeration rate was 1 vvm. After 18 hours of reaction, the water quality of the water sample treated by the original sewage station sludge was BOD5 20mg / L, COD 40mg / L, total nitrogen 20mg / L, and ammonia nitrogen 6mg / L; the water quality of the water sample treated with this patent domesticated sludge was BOD5 6mg / L L, COD 20mg / L, total nitrogen 7mg / L, ammonia nitrogen 3mg / L; using the mixed bacterial agent of the present invention, the overall efficiency is increased by about 50%.

Embodiment 3

[0046] Under special circumstances, the raw water quality of the domestic sewage of Chongzhao Village Ecological Demonstration Village in Huzhou City is BOD 5550 mg / L, COD 1000 mg / L, total nitrogen 60 mg / L, and ammonia nitrogen 25 mg / L. Take 1000 ml each and place them in a glass container. The village sewage treatment station sludge and the domesticated sludge according to the method of Example 1 are treated in aerobic stage, and the aeration rate is 1 vvm. After 24 hours of reaction, the water quality of the water sample treated by the original sewage station sludge is BOD5 50mg / L, COD 100mg / L, total nitrogen 30mg / L, and ammonia nitrogen 15mg / L; the water quality of the water sample treated with this patent domesticated sludge is BOD5 8mg / L L, COD 30mg / L, total nitrogen 8mg / L, ammonia nitrogen 4mg / L; impact resistance has been verified.

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Abstract

The invention provides a high-efficiency denitrification mixed bacterial agent and application thereof, and a novel bacterial strain capable of efficiently denitrifying obtained by screening. The high-efficiency denitrification mixed bacterial agent consists of CCTCC No:M 2010001 and nitrosobacteria CCTCC No:M 2010002. The invention mainly has the advantages of providing a high-efficiency denitrification mixed bacterial agent, greatly improving the efficiency for treating sewage, not causing secondary pollution, and having significance for improving ecological environment.

Description

(1) Technical field [0001] The purpose of the present invention is to provide a mixed bacterial agent for high-efficiency denitrification and its application, as well as new strains that can be screened for high-efficiency denitrification. (2) Background technology [0002] With the ever-expanding urban population and the continuous development of industry and agriculture, water pollution accidents occur frequently, seriously endangering the health and lives of people and animals. Many lakes and reservoirs have caused eutrophication of water bodies due to the discharge of nitrogen and phosphorus, which seriously threatens human production, life and ecological balance. Ammonia nitrogen is one of the main factors causing eutrophication of water bodies. In order to meet the continuous improvement of the public's environmental quality requirements, the country has formulated more and more stringent emission standards for nitrogen. The research and development of economical and effic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C02F3/34C02F3/02C12R1/01
CPCY02W10/10
Inventor 郑展望许敏伟
Owner ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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