Method for producing probiotic single cell protein by using soybean molasses

A single-cell protein and soybean molasses technology, which is applied in the field of soybean molasses production of probiotic single-cell protein, can solve problems such as affecting the development of enterprises, difficult to handle soybean molasses, etc., so as to improve the feed conversion rate, inhibit the growth of intestinal pathogens, The effect of high protein content

Inactive Publication Date: 2011-02-09
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a method for producing prebiotic single-cell protein with soybean molasses, which is used to solve the problem that the waste soybean molasses produced by soybean protein concentrate is difficult to handle and affects the development of enterprises

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dilute the soybean molasses with tap water to a mass percentage concentration of 20%, adjust the pH to 3.5 with phosphoric acid, take a 50 mL supernatant and put it into a 500 mL Erlenmeyer flask after bathing in a constant temperature water bath at 60°C for 2 hours, and continue diluting the soybean molasses supernatant with tap water to The mass percent concentration is 3%, the pH is adjusted to 5.0 with 4 mol / L sodium hydroxide, and sterilized at 121° C. for 15 minutes. After cooling to 28°C, add Candida tropicalis CGMCC2.587 seed culture solution, the amount of yeast seed culture solution added is 2% of the volume of soybean molasses medium, at 28°C, aerobic culture for 20h, and then add Lactobacillus acidophilus CGMCC1 .1854 seed culture solution, the addition of Lactobacillus acidophilus seed culture solution is 1% of the soybean molasses culture medium volume, 28 ℃ static culture 16h; then 5000r / min centrifugal 15min, vacuum-dry to constant weight at 40 ℃, obtain ...

Embodiment 2

[0022] The soybean molasses was diluted with tap water to a concentration of 18% by mass, the pH was adjusted to 4.0 with phosphoric acid, and the supernatant was taken after a constant temperature water bath at 70° C. for 1 hour. The soybean molasses supernatant was further diluted with tap water to a concentration of 5% by mass, adjusted to pH 5.0 with 4 mol / L sodium hydroxide, and sterilized at 121° C. for 18 minutes. After cooling to 30°C, add Candida tropicalis CGMCC2.587 seed culture solution, the amount of yeast seed culture solution added is 2% of the volume of soybean molasses medium, at 30°C, aerobic culture for 24h, and then add Lactobacillus acidophilus CGMCC1 .1854 seed culture solution, the addition amount of the Lactobacillus acidophilus seed culture solution is 1% of the volume of the soybean molasses medium, and cultured at 30° C. for 18 hours. Then 4000r / min centrifugal 20min, vacuum drying to constant weight at 40 ℃, the dry weight that obtains prebiotic sin...

Embodiment 3

[0024] Dilute the soybean molasses with tap water to a concentration of 15% by mass, adjust the pH to 3.5 with phosphoric acid, and take a supernatant after a constant temperature water bath at 80° C. for 30 minutes. Continue to dilute the soybean molasses supernatant with tap water to a mass percentage concentration of 7%, add yeast extract and potassium dihydrogen phosphate to the diluted supernatant, and the addition of yeast extract is the diluted supernatant 1.0% by weight, potassium dihydrogen phosphate is added in an amount of 0.10% by weight of the diluted supernatant. Adjust the pH to 5.5 with 4mol / L sodium hydroxide, and sterilize at 121°C for 15 minutes. After cooling to 30°C, add Candida tropicalis CGMCC2.587 seed culture solution, the amount of yeast seed culture solution added is 5% of the volume of soybean molasses medium, at 30°C, aerobic culture for 24h, and then add Lactobacillus acidophilus CGMCC1 .1854 seed culture solution, the addition amount of the Lact...

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Abstract

The invention relates to a method for producing probiotic single cell protein by using soybean molasses and belongs to a method for producing single cell protein in the technical field agricultural product processing. In the method for producing probiotic single cell protein by using soybean molasses, the probiotic single cell protein feed is produced by using Candida tropicalis CGMCC2.587 and probiotic lactic acid bacterium lactobacillus acidophilus CGMCC1.1854, which are obtained by amplifying culture and by removing impurities from soybean molasses, regulating ingredients, sterilizing, fermenting at two stages, centrifuging at a speed of 4,000 to 5,000 r / min for 15 to 20 minutes, and drying under vacuum at the temperature of below 40 DEG C. In the invention, the nutrients of the soybean molasses are fully utilized, the soybean molasses concentration is low, the production cost is low, the mycelium growth rate is high, the protein content of the probiotic single cell protein feed is high, high-protein feed, of which the mass percentage content of the protein is over 50 percent, can be obtained by a short period of fermentation, and the produced single cell protein feed contain rich active probiotic lactic acid bacteria.

Description

1. Technical field: [0001] The invention relates to a production method of a single-cell protein in the technical field of agricultural product processing, in particular to a method for producing a prebiotic single-cell protein with soybean molasses. 2. Background technology: [0002] Soybean molasses is the product of alcohol-soluble parts in the process of producing concentrated soybean protein. It is a product of concentrated soybean protein production. It has a brown-yellow viscous liquid appearance and a special fragrance. Because it is rich in sugar substances, its color and fluidity are similar to honey, so it is named soybean molasses. Soybean molasses contains various nutrients in soybeans. The concentration of soybean molasses is 65%-72%, which contains 5%-8% protein, 30%-35% sugar, 0.5%-2% soybean iso Flavonoids, 3% to 6% saponins, 4% to 8% lipids and about 4% ash. The sugar contained in soybean molasses includes soybean oligosaccharides, including 4.27% raffino...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K10/12A23K10/33A23K20/147A23K40/00
CPCY02P60/87
Inventor 高玉荣李大鹏刘洋
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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