Duck infectious serositis inactivated vaccine emulsification method
An inactivated vaccine and infectious technology, which is applied in the emulsification field of duck infectious serositis inactivated vaccine, can solve the problems of vaccine quality impact, emulsification production process without formal procedures, and the safety of inoculated animals, etc., to achieve stability Good, the qualified rate of dosage form is improved, and the effect of uniform color and luster
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Embodiment 1
[0016] Preparation of 1% lysed hemocyte whole blood N-J synthetic medium:
[0017] Prepare 100ml of 1% lysed blood cell whole blood N-J synthetic medium: tryptone: 1.9g; yeast extract: 0.7g; beef extract: 0.7g; hydrolyzed milk protein: 0.7g; glucose: 0.4g; NaCl: 0.5g ; Potassium dihydrogen phosphate: 0.3g; Dipotassium hydrogen phosphate: 0.3g; Na 2 HPO 4 12H 2 O: 0.9g; (NH 4 ) 2 SO 4 : 0.2g; NH 4 Cl: 0.03g; add water to 100mL; sterilize the above-mentioned components at 121°C for 15 minutes, and when the sterilized components are cooled to below 40°C, add 1ml of lysed blood cells and whole blood to the above-mentioned basal medium; After shaking well, the finished N-J synthetic medium can be obtained.
[0018] The whole blood of the added lysed hemocytes is aseptically collected from healthy bovine blood in non-epidemic areas by jugular vein blood collection or carotid artery bloodletting and aseptically defibrillated, frozen and thawed at -20°C and room temperature, an...
Embodiment 2
[0020] Preparation of duck infectious serositis inactivated vaccine vaccine preparation liquid:
[0021] 1 strain: Serum Type I Riemerella anatipestifer RA-CH-I strain.
[0022] 2 Culture medium: 1% lysed blood cell whole blood N-J synthetic medium, 10% serum nutrient agar plate, lysed whole blood and inspection medium are all produced in strict accordance with the appendix of "Chinese Veterinary Pharmacopoeia" and tested after passing the test.
[0023] 3 methods
[0024] 3.1 Propagation and identification of first-class seeds
[0025] Put the freeze-dried basal strain of serotype I Riemerella anatipestifer RA-CH-I into 5ml of nutrient broth containing 10% calf serum, and culture it at 37°C for 24 hours; then streak inoculated in 2 On the nutrient agar plate containing 10% calf serum, according to the standard [smooth surface, slightly protruding, round, creamy colonies, colony diameter 1.2 ~ 1.7mm; bacterial smears can be seen as single (occasionally in pairs) or filament...
Embodiment 3
[0039] Screening of the best emulsification conditions:
[0040] Material:
[0041] 1.1 Bacterial liquid: Riemerella anatipestifer RA-CH-I strain is used for seedling preparation.
[0042] 1.2 White oil for injection was purchased from Hangzhou Oil Refinery.
[0043] 1.3 Siben-80 was purchased from Chengdu Dongjin Chemical Reagent Factory.
[0044]1.4 Aluminum stearate was purchased from Shanghai Yuanhang Chemical Reagent Factory.
[0045] 1.5 Tween-80 was purchased from Chengdu Dongjin Chemical Reagent Factory.
[0046] 1.5 Formaldehyde solution AR was purchased from Chengdu Honghe Reagent Factory.
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