Energy-saving type culturing method of chaliotis diversicolor
A culture method, the technology of nine-hole abalone, applied in the directions of microorganism-based methods, biochemical equipment and methods, fish farming, etc., can solve the adverse effects of immunity, drug resistance and drug residues, health hazards in aquaculture, etc. It can prevent the bacterial disease of nine-hole abalone, improve the survival rate of breeding, and improve the immunity.
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[0030] (1) Preparation of the Bdellovibrio bruplasma liquid used for growing Haliotis diversicolor aquatilis
[0031]Inoculate 0.5mL10 in an Erlenmeyer flask containing 100mL nutrient broth liquid medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH7.4±0.2) 7 cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, strain number: GIM1.172, sourced from the Guangdong Provincial Microbial Culture Collection Center), 200rpm, 35°C shaker culture for 12 hours, the culture solution was centrifuged at 4°C, 5000rpm 15min, discard the supernatant. Use 5mL of normal saline to suspend and precipitate the bacteria, and then add it to a liquid medium containing 100mL DNB (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, dissolve in 1000mL distilled water, pH 7.2~7.6) into the Erlenmeyer flask, add 1mL containing 10 3 Bdellovibrio BDFM05 in pfu / mL (CCTCC M209172). Incubate on a constant temperature shaker at 250 rpm at 28°C for 36 hours. Centrifuge the cul...
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