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Energy-saving type culturing method of chaliotis diversicolor

A culture method, the technology of nine-hole abalone, applied in the directions of microorganism-based methods, biochemical equipment and methods, fish farming, etc., can solve the adverse effects of immunity, drug resistance and drug residues, health hazards in aquaculture, etc. It can prevent the bacterial disease of nine-hole abalone, improve the survival rate of breeding, and improve the immunity.

Inactive Publication Date: 2013-10-30
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] During the breeding process of abalone, the use of antibiotics has played a positive role in inhibiting the reproduction of pathogenic microorganisms and reducing the poisonous effect of their metabolic toxins on abalone. However, the long-term use of antibiotics, especially the abuse of antibiotics, has gradually revealed its various disadvantages
For example, the imbalance of the normal flora in the gastrointestinal tract of abalone will have adverse effects on the immunity of the body of the abalone, resulting in side effects such as drug resistance and drug residues, which will bring potential harm to the healthy development of the aquaculture industry and human health.
In addition, in the current industrialized land-based abalone farming in my country, the main method is to extract seawater from the outside and enter the culture pond after sand filtration and sedimentation, or to extract sand well water for running water culture, and the waste water is directly discharged into the sea area through the pipeline. This direct pumping and direct discharge method It has the disadvantages of high energy consumption and great pollution to sea areas, which not only increases the cost of breeding, but also becomes a major hidden danger of diseases in the future

Method used

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  • Energy-saving type culturing method of chaliotis diversicolor
  • Energy-saving type culturing method of chaliotis diversicolor
  • Energy-saving type culturing method of chaliotis diversicolor

Examples

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Embodiment 1

[0030] (1) Preparation of the Bdellovibrio bruplasma liquid used for growing Haliotis diversicolor aquatilis

[0031]Inoculate 0.5mL10 in an Erlenmeyer flask containing 100mL nutrient broth liquid medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH7.4±0.2) 7 cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, strain number: GIM1.172, sourced from the Guangdong Provincial Microbial Culture Collection Center), 200rpm, 35°C shaker culture for 12 hours, the culture solution was centrifuged at 4°C, 5000rpm 15min, discard the supernatant. Use 5mL of normal saline to suspend and precipitate the bacteria, and then add it to a liquid medium containing 100mL DNB (nutrient broth 0.8g, casein acid hydrolyzate 0.5g, yeast extract 0.1g, dissolve in 1000mL distilled water, pH 7.2~7.6) into the Erlenmeyer flask, add 1mL containing 10 3 Bdellovibrio BDFM05 in pfu / mL (CCTCC M209172). Incubate on a constant temperature shaker at 250 rpm at 28°C for 36 hours. Centrifuge the cul...

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Abstract

The invention discloses an energy-saving type culturing method of chaliotis diversicolor, comprising the following steps: taking deep seawater without pollution in sea areas as culturing water; selecting and putting health chaliotis diversicolor in good condition in to a culturing pond; spraying bacteria liquid of bdellovibrio bacteriovorus and leech plasmids into the culturing pond, and leading the concentration of the bdellovibrio bacteriovorus and the leech plasmids in water of the pond to be 10-107pfu / mL, throwing baits once every day, controlling the bait throwing amount at each time to be 5%-15% of the body weight of the chaliotis diversicolor; before being used, soaking the baits with the bacteria liquid of bdellovibrio bacteriovorus and leech plasmids with the concentration of 10-107pfu / mL; and changing the water in the pond every 5-30 days and spraying the bacteria liquid of bdellovibrio bacteriovorus and leech plasmids after the water is changed at each time. The culturing method has obvious promoting function to the growth of the chaliotis diversicolor, improves the immunity and survival rate of the chaliotis diversicolor, and improves the water quality of the culturing environment. As the bdellovibrio bacteriovorus and leech plasmids has no toxic and side effects, the energy-saving type culturing method is suitable for large-scale industrial culture of the chaliotis diversicolor.

Description

technical field [0001] The invention belongs to the technical field of seawater shellfish culture, and in particular relates to a new energy-saving method for cultivating Haliotis diversicolor aquatilis. Background technique [0002] Abalone is one of the eight sea treasures in my country, which has high economic and medicinal value. my country's abalone breeding started in the 1970s, and had formed large-scale production in the mid-1980s. Since then, it has developed rapidly, and the abalone farming industry in coastal areas has formed a relatively large scale. However, with the aggravation of environmental pollution, the expansion of aquaculture scale and the improvement of intensification, abalone diseases have occurred one after another, and there is a trend of increasing year by year, which has posed a serious threat to the development of abalone industry in my country. Especially for abalone, in recent years, there have been frequent disease problems in abalone cultiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A23K1/18A23K1/16C12N1/20C12R1/01A23K10/18A23K50/80
CPCY02A40/81
Inventor 蔡俊鹏陈小红
Owner SOUTH CHINA UNIV OF TECH
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