Method for extracting grease from wet alga mud by using mixed organic solvent
A technology of organic solvents and mixed solvents, applied in the direction of fat oil/fat production and fat production, can solve the problems of large amount of alcohol, low solubility, low solubility, etc., to save drying energy consumption, high oil extraction rate, simple separation Effect
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[0026] Example 1
[0027] Weigh 100g of Pseudochlorococcum wet algae mud (60% water content, 33.4% initial total lipid content), and add 400ml of analytically pure ethanol (99% ethanol content) and n-hexane mixed liquid (the volume ratio of the two is 1 / 6) Put it in a stainless steel pressure reactor and seal, and pass N into the reactor 2 To 1.5MPa, heat to 110 ℃ and maintain the reaction for 1 hour, then take out the algae slurry, centrifuge at 6500 rpm for 10 min, remove the microalgae cell residues, let the supernatant stand still, separate the upper layer of hexane phase, ethanol phase with 60ml of hexane After extraction twice, all n-hexane phases were combined, concentrated by rotary evaporation, and dried to obtain 11.2 g of algae oil, with a total lipid extraction rate of 83.8%.
Example Embodiment
[0028] Example 2
[0029] Weigh 100 g of Pseudochlorococcum wet algae mud (60% water content, 33.4% initial total fat content), and add 400 ml of a mixed liquid of industrial ethanol (85% ethanol content) and n-hexane (the volume ratio between the two is 1 / 3 ), placed in a high-pressure reactor and sealed, and pass N into the reactor 2 To 1.5MPa, heat to 110°C and react for 1 hour, then take out the algae slurry, centrifuge at 6500rpm for 10min, remove the microalgae cell residue, leave the supernatant to separate the upper n-hexane phase, and extract the ethanol phase with 100ml n-hexane Once, all the n-hexane phases were combined, concentrated by rotary evaporation, and dried to obtain 8.68 g of algae oil with a total lipid extraction rate of 65%.
Example Embodiment
[0030] Example 3
[0031] Weigh 100g of Pseudochlorococcum wet algae mud (60% water content, 33.4% initial total fat content), add 320ml of analytically pure ethanol and n-hexane mixed liquid (the volume ratio between the two is 1 / 5), and place it under high pressure The reactor is sealed, and N is introduced into the reactor 2 To 1.5MPa, heat to 100°C and react for 1 hour, then take out the algae slurry, centrifuge at 6500rpm for 10min to remove the microalgae cell residue, let the supernatant stand still, separate the upper n-hexane phase, and extract the ethanol phase with 60ml n-hexane Twice, all the n-hexane phases were combined, concentrated by rotary evaporation, and dried to obtain 10.69 g of algae oil with a total lipid extraction rate of 80%.
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