Preparation method of reagent board for rapidly detecting aflatoxin B1

A technology of aflatoxin and detection reagents, which is applied in the field of preparation of rapid detection reagent plates for detection of aflatoxin B1, can solve the problems of short life of reagents, unsafe operation, poor repeatability of measurement results, etc., and achieve high sensitivity and easy operation Fast and specific effect

Inactive Publication Date: 2011-06-08
杭州南开日新生物技术有限公司
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Problems solved by technology

However, the activity of the enzyme in the ELISA method is easily affected by the reaction conditions, which easily leads to poor repeatability of the measurement results.
In addition, the life o

Method used

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  • Preparation method of reagent board for rapidly detecting aflatoxin B1
  • Preparation method of reagent board for rapidly detecting aflatoxin B1
  • Preparation method of reagent board for rapidly detecting aflatoxin B1

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Embodiment Construction

[0029] The preparation of the reagent plate of the present invention includes the preparation of aflatoxin B1 carrier protein conjugate, the preparation of anti-aflatoxin B1 monoclonal antibody, the preparation of colloidal gold solution, the preparation of colloidal gold-labeled anti-aflatoxin B1 monoclonal antibody and the preparation of aflatoxin Assembly of mycotoxin immunocolloidal gold rapid detection reagent plate.

[0030] 1. Coupling of aflatoxin B1 to carrier protein

[0031] The immune antigen and coating antigen were prepared by coupling aflatoxin B1 with carrier protein (bovine serum albumin BSA and ovalbumin OVA) by carbodiimide method (EDC method).

[0032]Weigh 2 mg of aflatoxin B1 and 4 mg of CMO, dissolve in 400 μL of pyridine, and shake at 25°C in the dark. until the reaction is complete. The reaction product was freeze-dried, the resulting residue was dissolved in 3 mL of ultrapure water, and the pH value was adjusted to 8.0 with 0.1 mol / L sodium hydroxid...

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Abstract

The invention discloses a preparation method of a reagent board for rapidly detecting aflatoxin B1, belonging to the field of mycotoxin detection and particularly relating to a preparation method of a reagent board for rapidly detecting aflatoxin B1 in grains and edible oil. The reagent board comprises an upper plastic template, a lower plastic template and a back lining; a sample pad, a colloidal gold combining pad, a nitrocellulose membrane and a water sucking pad are sequentially and tightly attached on the back lining; a detection line and a quality control line are sequentially sprayed on the nitrocellulose membrane in the direction of from the sample pad to the water sucking pad, and a reaction result can be represented by using a color visible to the unaided eye. The reagent board can be used for performing semi-quantitative and intuitive detection, the whole operation process only takes 40 minutes, no any expensive experimental device is needed for assistance, thereby the invention is good for sample screening on a large scale and suitable for industry and business departments, inspection and quarantine organizations and grain and oil production and processing enterprises to carry out rapid detection on the aflatoxin in the grains and the edible oil on a large scale.

Description

technical field [0001] The invention relates to a method for preparing a rapid detection reagent plate for detecting aflatoxin B1, in particular to a method for preparing an immunocolloidal gold rapid detection reagent plate for detecting aflatoxin B1 in grains and edible oils. Background technique [0002] Aflatoxin (AFT) is a mycotoxin (Mycotoxin), which is mainly produced by Aspergillus flavus and Aspergillus parasiticus, and other Aspergillus such as Phytophthora, Penicillium, Fusarium, Rhizopus, etc. can also produce AFT, AFT Commonly found in peanuts and their products, corn, cottonseed, and some nut foods and feeds. AFT includes more than 10 kinds of B1, B2, G1 and G2, among which aflatoxin B1 is the most common and the most toxic. B1 has a wide distribution range and can cause acute poisoning death of humans (especially children) and various domesticated animals. It can also cause teratogenic, carcinogenic, and mutagenic effects. toxicity. Therefore, it poses a gr...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/558G01N33/532
Inventor 张少恩桑丽雅周崇盈
Owner 杭州南开日新生物技术有限公司
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