Preparation method of reagent board for rapidly detecting aflatoxin B1

Abstract

The invention discloses a preparation method of a reagent board for rapidly detecting aflatoxin B1, belonging to the field of mycotoxin detection and particularly relating to a preparation method of a reagent board for rapidly detecting aflatoxin B1 in grains and edible oil. The reagent board comprises an upper plastic template, a lower plastic template and a back lining; a sample pad, a colloidal gold combining pad, a nitrocellulose membrane and a water sucking pad are sequentially and tightly attached on the back lining; a detection line and a quality control line are sequentially sprayed on the nitrocellulose membrane in the direction of from the sample pad to the water sucking pad, and a reaction result can be represented by using a color visible to the unaided eye. The reagent board can be used for performing semi-quantitative and intuitive detection, the whole operation process only takes 40 minutes, no any expensive experimental device is needed for assistance, thereby the invention is good for sample screening on a large scale and suitable for industry and business departments, inspection and quarantine organizations and grain and oil production and processing enterprises to carry out rapid detection on the aflatoxin in the grains and the edible oil on a large scale.

Description

technical field [0001] The invention relates to a method for preparing a rapid detection reagent plate for detecting aflatoxin B1, in particular to a method for preparing an immunocolloidal gold rapid detection reagent plate for detecting aflatoxin B1 in grains and edible oils. Background technique [0002] Aflatoxin (AFT) is a mycotoxin (Mycotoxin), which is mainly produced by Aspergillus flavus and Aspergillus parasiticus, and other Aspergillus such as Phytophthora, Penicillium, Fusarium, Rhizopus, etc. can also produce AFT, AFT Commonly found in peanuts and their products, corn, cottonseed, and some nut foods and feeds. AFT includes more than 10 kinds of B1, B2, G1 and G2, among which aflatoxin B1 is the most common and the most toxic. B1 has a wide distribution range and can cause acute poisoning death of humans (especially children) and various domesticated animals. It can also cause teratogenic, carcinogenic, and mutagenic effects. toxicity. Therefore, it poses a gr...

AI Technical Summary

Problems solved by technology

However, the activity of the enzyme in the ELISA method is easily affected by the reaction conditions, which easily leads to poor repeatability of the measurement results.

In addition, the life of ELISA reagents is short, so low temperature storage is required, and this method needs to be exposed to aflatoxin standard substances when preparing a standard curve, which is unsafe to operate

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