Method for producing succinic acid by fermentation of crop straw
A technology of crop straw and succinic acid is applied in the field of comprehensive utilization of crop straw, which can solve the problems of unutilized, succinic acid consumes large amounts of food, and threatens human survival, and achieves the effects of low production cost and high utilization rate.
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Embodiment 1
[0023] Example 1 Preparation of Straw Enzymatic Hydrolysis Sugar Liquid
[0024] 1) crushing and washing the corn stalks;
[0025] 2) Soda cooking of corn stalks: boil the crushed and washed crop stalks in 16g / L NaOH solution at 100°C for 2 hours;
[0026] 3) Solid-liquid separation: solid-liquid separation of the soda cooking solution to obtain soda cooking slag;
[0027] 4) Enzymolysis of alkali boiled residue: use 20IU cellulase, 10IU cellobiase, and 15IU xylanase per gram of alkali boiled residue; during the enzymolysis process, the pH is 6.0, the temperature is 40°C, and the enzymolysis time is 40 hours , to obtain enzymatic solution;
[0028] 5) Solid-liquid separation: solid-liquid separation of enzymatic hydrolysis liquid to obtain straw enzymatic hydrolysis sugar liquid rich in glucose, xylose and a small amount of other miscellaneous sugars and enzymatic hydrolysis slag rich in lignin.
Embodiment 2
[0029] Example 2 Preparation of Straw Enzymolysis Sugar Liquid
[0030] 1) crushing and washing the corn stalks;
[0031] 2) Alkaline cooking of corn stalks: boil the crushed and washed crop stalks in 20g / L sodium hydroxide solution at 120°C for 1 hour;
[0032] 3) Solid-liquid separation: solid-liquid separation of the soda cooking solution to obtain soda cooking slag;
[0033] 4) Enzymolysis of alkali boiled residue: use 30IU cellulase and 20IU xylanase per gram of alkali boiled residue; during the enzymolysis process, the pH is 6.5, the temperature is 45°C, and the enzymolysis time is 45 hours to obtain an enzymolysis solution;
[0034] 5) Solid-liquid separation: solid-liquid separation of enzymatic hydrolysis liquid to obtain straw enzymatic hydrolysis sugar liquid rich in glucose, xylose and a small amount of other miscellaneous sugars and enzymatic hydrolysis slag rich in lignin.
Embodiment 3
[0035] Embodiment 3 prepares xylose mother liquor
[0036] washing the corncob;
[0037] Acid hydrolysis of corncobs: acid-treat the washed corncobs in 12g / L sulfuric acid at 125°C for 2 hours;
[0038] Solid-liquid separation: the corncob acid treatment liquid is separated from solid-liquid to obtain acidolysis solution and acidolysis residue;
[0039] Crystallization: Purify, concentrate and crystallize the acid solution to obtain xylose and xylose mother liquor.
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