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Bactericide

A fungicide and solvent technology, applied in the field of volatile mixtures, can solve problems such as poor environmental degradation ability, reduction in the number of natural enemies of harmful organisms, and destruction of ecological balance.

Active Publication Date: 2011-09-14
ZHEJIANG DAYANG BIOTECH GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemically synthesized agents has brought a series of serious consequences: 1) High residues: due to the carcinogenicity and toxicity of chemically synthesized agents, including various raw materials, it is not easy to get rid of them when entering animals and plants, and the environmental degradation ability is poor, resulting in pesticide residues High; 2) Destruction of ecological balance: long-term evolution and adaptation of organisms, the relative number of various organisms in nature has reached a dynamic balance, and the use of chemical agents has greatly reduced the number of natural enemies of harmful organisms; 3) The emergence of drug resistance: pathogenic bacteria and insects in nature The gene of the class has a strong ability to mutate, and irrational drug use and blind drug use promote the rapid emergence of drug resistance

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0039] Example 1, Analysis of VOCs produced by endophytic fungus Muscodor sp. strain ZJLQ023

[0040] The VOCs produced by the endophytic fungus Muscodor sp. strain ZJLQ023 were analyzed by solid phase extraction / gas chromatography / mass spectrometry (Solid phase microextraction / Gas chromatograph / Mass spectrometry, SPME / GC / MS). Inoculate Muscodor sp. strain ZJLQ023 on PDA medium, culture at 25°C for 3-5 days, cut out the bacteria cake and transfer it to PDA or MEA medium, seal the disposable plastic culture dish with parafilm, and place in a 25°C biochemical incubator After culturing for a certain period of time (3d, 5d, 10d), use a fine needle to drill a small hole from the edge of the Petri dish. Insert the solid-phase microextraction stainless steel protective needle tube into the GC / MS sampling chamber, push out the extraction head, expose the extraction head to the GC vaporization chamber, activate at 240°C for 20 minutes, and then retract the extraction head to the protec...

Embodiment 2

[0065] Example 2, VOCs produced by Muscodor sp. strain ZJ LQ023 prevent and control post-harvest fruit rot test

[0066] In order to test the VOCs produced by Muscodor sp. bacterial strain ZJLQ023 on the prevention and control of post-harvest fruit rot, the examples are as follows: the Muscodor sp. bacterial strain ZJLQ023 will be used to control the post-harvest fruit decay on the PDA medium or the prepared active carrier preparation rot problem. Specifically, pick fruits that have no wounds or pests after harvest (Xinjiang pears or peaches), put them in a fruit preservation box (can be sealed), gently scratch the wound with a scalpel, and insert brown rot bacteria into the wound (Monilinia fructicola), and apply wet filter paper on the wound for moisturizing, and place the active carrier preparation Muscodor sp. strain ZJLQ023 grown on the PDA medium or prepared in the fruit preservation box for inhibiting the growth of pathogenic bacteria , add a cover and seal it, put it ...

Embodiment 3

[0067] VOCs that embodiment 3, Muscodor sp. bacterial strain ZJLQ023 produce prevents and treats seedling disease

[0068] The control effect of VOCs produced by Muscodor sp. strain ZJLQ023 on seedling diseases was determined by radish seedling growth method. Put the crushed sorrel into a test tube with a diameter of 50mm and a height of 400mm, the height of which is about 10cm, and sterilize with moist heat at 121°C for 20min. Insert pathogenic bacterium ultimate Pythium cake (diameter 8mm) 5-6 pieces in each test tube, cover with a stone, sprinkle 10 seeds on it (Short-leaf No. 13 radish, Guangdong Provincial Fine Variety Introduction Service Company provides), Then cover it with a stone. Muscodor sp. strain ZJLQ023 active carrier preparation (packaged with gauze outside) was hung in the test tubes of the test group, and only the pathogenic bacteria were connected as the blank control, and the test tubes were sealed with breathable soft plugs. Move them into an artificial ...

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Abstract

The invention discloses a bactericide which comprises 2-methyl-methyl propionate, 2-methyl propioric acid, 1-vinyl-1-methyl-2,4-di(1-methyl vinyl)-cyclohexane, carypohyllene, 2, 6-dimethyl-6-(4-methyl-3-pentene)bicyclo[3.1.1]heptyl-2-alkenyl, 1,2,3,4,5,6,7,8-octahydro-1,4-dimethyl-7-(1-methyl ethylidene)azulene, 1,2,3,4,4a,5,6,8a-octahydro-4a, 8-dimethyl-2-(1-methyl ethyl)naphthaline, 3,3,7,11-tetramethyl tricyclo[6.3.0.0(2,4)]undecarbon-8-alkene and the like. The bactericide can be used for inhibiting or killing pathogenic microorganisms in the crop production process, mildew microorganisms of building materials and pathogenic microorganisms in human or animal living environments.

Description

[0001] The present invention is a divisional application of "Muscodor plant endophytic fungus and its use and fungicide" with the filing date of 2009.09.30 and the application number of 200910153513.5. technical field [0002] The present invention relates to the fields of microbiology and fungicides, in particular, the present invention relates to a new Muscodor plant endophytic fungus and a volatile mixture based on or derived from it and its use. Background technique [0003] Fungi are a huge family of microorganisms. Fungi are closely related to humans. Fungi can be used to manufacture important products in industry and agriculture such as medicine, food, and chemical industry. The famous penicillins and cephalosporins are one of the most precious drugs brought to the medical world by fungi so far, and they are also the most important antibiotics at present. They are widely used in the treatment of bacterial infections and have saved thousands of human lives. life. [0...

Claims

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Application Information

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IPC IPC(8): A01N37/02A01N31/06A01N27/00A01P3/00
Inventor 王国平章初龙林福呈毛黎娟周转忠
Owner ZHEJIANG DAYANG BIOTECH GROUP
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