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Method for preparing tobacco stem cellulose through microbial solid fermentation process

A technology of microbial fermentation and solid-state fermentation, applied in the field of tobacco processing, can solve problems such as bad odor, and achieve the effects of reducing equipment investment, improving comprehensive quality and simple process

Active Publication Date: 2013-04-24
SHANGHAI TOBACCO GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The high content of protein remaining in the tobacco flakes makes the smoke smell of burnt protein, which makes the smoke smell bad

Method used

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  • Method for preparing tobacco stem cellulose through microbial solid fermentation process
  • Method for preparing tobacco stem cellulose through microbial solid fermentation process
  • Method for preparing tobacco stem cellulose through microbial solid fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Effect of Fermentation Time on Tobacco Stem Yield and Residual Pectin Content

[0061] Solid-state fermentation method: add 50g of tobacco stems to a sterilized 1000ml Erlenmeyer flask, add 30mL of water, inoculate 20mL of Aspergillus niger secondary seed solution, and place them in a solid-state incubator at 30°C for 32h, 38h, 44h, 50h, 56h respectively , 62h, after the end of fermentation, soak in 0.1mol / L, pH 4.4 citric acid buffer solution for 24h, rub the fermented tobacco stems into pieces, wash them with clean water, filter through a sieve to remove bacteria and sundries, and place the remaining tobacco stem residues in Dry at 40°C, measure its weight, and calculate the yield.

[0062] The impact of different fermentation times on the yield of tobacco stem residue is shown in Table 1, and the tobacco stems in fermentation are as follows: Figure 4 shown. Tobacco stem residues fermented at different times by Figure 5 shown. As can be seen from Table 1, with t...

Embodiment 2

[0068] Comprehensive experiment of Aspergillus niger solid-state fermentation of tobacco stems: A small-scale solid-state fermentation tank was used to ventilate and ferment tobacco stems, and Aspergillus niger was used as the strain.

[0069] (1) Preparation of spore suspension:

[0070] Aspergillus niger was selected as the fermentation microorganism, PDA slant medium was used, and after streak inoculation, it was cultured at 30°C for 5 days to obtain mature spores of Aspergillus niger. Add 60 mL of sterile water to the cultured eggplant bottle (8×24cm) on the slant, scrape off the spores with an inoculation shovel; transfer the spore suspension to a sterile Erlenmeyer flask containing glass beads; break up to obtain Spore suspensions in which the concentration of spores in the spore suspension is not less than 10 7 individual / mL.

[0071] (2) Preparation of Aspergillus niger primary, secondary, and tertiary seed solutions:

[0072] In a 1L Erlenmeyer flask sterilized at ...

Embodiment 3

[0088] Comprehensive experiment of Aspergillus niger solid-state fermentation of tobacco stems: A small-scale solid-state fermentation tank was carried out to ferment tobacco stems with ventilation, and Aspergillus niger was used as the strain.

[0089] (1) Preparation of spore suspension:

[0090] Aspergillus niger was selected as the fermentation microorganism, PDA slant medium was used, and after streak inoculation, it was cultured at 32°C for 5 days to obtain mature spores of Aspergillus niger. Add 60 mL of sterile water to the cultured eggplant bottle (8×24cm) on the slant, scrape off the spores with an inoculation shovel; transfer the spore suspension to a sterile Erlenmeyer flask containing glass beads; break up to obtain Spore suspension, wherein the concentration of spores in the spore suspension is 10 7 individual / mL.

[0091] (2) Preparation of Aspergillus niger primary, secondary, and tertiary seed solutions:

[0092] In a 1L Erlenmeyer flask sterilized at 121°C...

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Abstract

The invention belongs to the technical field of tobacco processing and relates to a method for preparing basic material of a tobacco thin sheet, namely tobacco stem cellulose through the microbial solid fermentation process. Microbes including high-yield prolease, pectolase, ligninase, amylase and other enzymes are selected, and tobacco stems, tobacco smalls and crumbled tobacco are taken as raw materials for preparing microbial seeds, the prepared microbial seeds are inoculated into the tobacco stems for performing solid fermentation, soaking the tobacco stems after fermentation in buffer solution, further performing enzymolysis for degrading the tobacco stems after the enzymolysis, proteins, lignin, pectic substances and other macromolecular substances into micromolecular substances which are easy to be separated from cellulose type substances in the tobacco stems, and further stirring, pulping, filtering and separating for getting water-soluble extract and water-insoluble celluloseof the tobacco stems. The method has the advantages of being simple in process, saving energy, reducing equipment investment and improving the synthetic quality of the tobacco thin sheet, and can greatly reduce the residual quantity of the proteins, pectin, starch and other macromolecules in the tobacco thin sheet.

Description

technical field [0001] The invention belongs to the technical field of tobacco processing, and relates to a method for preparing tobacco stem cellulose, a basic material of tobacco flakes, by a microbial solid-state fermentation method. Background technique [0002] The production method of tobacco flakes at present has roll pressing method, thick slurry method and papermaking method. The papermaking method is the most popular, such as figure 1 The production process of paper-making tobacco sheet is shown. However, the energy consumption of the mechanical refining and extraction process in the papermaking method is high; some extraction technologies need to consume organic solvents. Not only increase the cost, but also pollute the environment. The protein content in tobacco flakes is too high, the aroma is insufficient, and the miscellaneous gas is relatively large. The high content of protein remaining in the tobacco flakes makes the smoke smell of burnt protein and giv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A24B3/14A24B15/20
Inventor 许赣荣汤朝起胡慧东于兴伟盛科
Owner SHANGHAI TOBACCO GRP CO LTD
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