Method for preparing gingko and tremella mesenterica fruit wine through fermentation by taking gingko as substrate
A technology of golden ear fruit wine and ginkgo, applied in the biological field, can solve the problems of not representing golden ear medicinal varieties, difficult to obtain golden ear products, difficult to obtain golden ear resources, etc., and achieves improved medicinal value, suitable taste, and radiation resistance. effect of function
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Embodiment 1
[0037] A method for preparing ginkgo golden fungus fruit wine by mixed submerged fermentation of yeast-like spores of golden fungus and host Bacillus chamomile using ginkgo fruit as a substrate, comprising the following steps:
[0038] (1) Inoculate the slant strain of Auricularia cerevisiae spores into PDA medium (Agricultural Microbiology Center of China Microbiological Culture Collection Management Committee, China Agricultural Strain Catalog, 1991 edition: page 93), culture at 18°C, and culture Time 120 hours;
[0039] (2) Inoculate the slant strains of the host pilus bacilli into the PDA medium (the Agricultural Microbiology Center of the China Microbiological Culture Collection Management Committee, the Chinese Agricultural Strain Catalog, 1991 edition: 93 pages), and the culture temperature is 23 ° C. Culture time 120 hours;
[0040] (3) Transfer the yeast-like spore slant strain obtained in step (1) into a triangular flask equipped with a shaker flask seed medium,...
Embodiment 2
[0049] A method for preparing ginkgo golden fungus fruit wine by mixed submerged fermentation of yeast-like spores of golden fungus and host Bacillus chamomile using ginkgo fruit as a substrate, comprising the following steps:
[0050] (1) Inoculate the slant strain of Auricularia cerevisiae spores into the PDA medium, cultivate at 28°C, and cultivate for 48 hours;
[0051] (2) Inoculate the slant strain of the host pili basilarus into the PDA medium, cultivate at 28°C, and cultivate for 148 hours;
[0052] (3) Transfer the yeast-like spore slant strain obtained in step (1) into a triangular flask equipped with a shaker flask seed medium, at 28°C, with a rotation speed of 250 rpm, and a culture time of 75 hours; The above-mentioned golden ear yeast-like spore shake flask seed culture medium consists of (unit is g / L): glucose 50, bran 100 (clean liquid obtained by boiling juice and filtering), potassium dihydrogen phosphate 2, magnesium sulfate 2, add water to appropriate volu...
Embodiment 3
[0061] A method for preparing ginkgo golden fungus fruit wine by mixed submerged fermentation of yeast-like spores of golden fungus and host Bacillus chamomile using ginkgo fruit as a substrate, comprising the following steps:
[0062] (1) Inoculate the slant strain of Auricularia cerevisiae spores into the PDA medium, cultivate at 23°C, and cultivate for 96 hours;
[0063] (2) Inoculate the slant strain of the host pili basilarus into the PDA medium, cultivate at 25°C, and cultivate for 136 hours;
[0064] (3) Transfer the yeast-like spore slant strain obtained in step (1) into a triangular flask equipped with a shaker flask seed medium, at 25°C, with a rotation speed of 150 rpm, and a culture time of 66 hours; The above-mentioned golden ear yeast-like spore shake flask seed culture medium consists of (unit is g / L): glucose 40, bran 75 (clean liquid obtained by boiling juice and filtering), potassium dihydrogen phosphate 1, magnesium sulfate 0.5, add water to appropriate vol...
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