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A kind of microbial agent for high-efficiency fermentation bed and preparation method thereof

A microbial agent and composite microbial agent technology, applied in the field of microbial agent for fermentation beds and its preparation, can solve the problems of land occupation, high cost, and large air pollution, and achieve pollution reduction, stable quality, and clear composition Effect

Inactive Publication Date: 2011-12-21
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, most of the traditional pig manure treatment technologies use composting fermentation, biogas fermentation, aerobic fermentation and drying technology, etc. Composting and fermentation treatment of manure takes up land, causing great air pollution to the environment, and biogas fermentation has seasonal limitations. The cost of aerobic fermentation and drying technology is relatively high, and there are certain limitations in practical application

Method used

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  • A kind of microbial agent for high-efficiency fermentation bed and preparation method thereof
  • A kind of microbial agent for high-efficiency fermentation bed and preparation method thereof
  • A kind of microbial agent for high-efficiency fermentation bed and preparation method thereof

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Experimental program
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Embodiment 1

[0050] Embodiment 1: preparation of composite bacterial agent

[0051] 1. Preparation of Bacillus subtilis fermentation product

[0052] Activation of slant strains: Store the slant strains in the refrigerator, aseptically inoculate them into fresh test tube slant medium, and culture at a constant temperature of 37°C overnight to make fresh test tube slant strains. Incline medium: peptone 1%, NaCl 0.5%, yeast extract 0.5%, agar 1.5%-2.0%, the rest is water, pH 7.2-7.4.

[0053] Shake flask seed culture: Take a ring of activated fresh slant strains and inoculate it into the shake flask seed liquid medium by aseptic operation, the filling volume is 100mL / 500mL, the rotation speed is 180-200rpm, and the temperature is 34-37℃ for 22-24h Finally, heat treatment in a water bath at 80°C for 10 minutes. The culture medium of the described shaking flask seeds does not add agar in the slant culture medium.

[0054] Fermentation tank culture: The medium loading in the fermentation tan...

Embodiment 2

[0068] Embodiment 2: Utilize the composite bacterial agent of the present invention to prepare the pig raising effect of fermentation bed

[0069] 1. Fermentation bed preparation

[0070] The fermented mattress material is composed of sawdust and rice husk in a weight ratio of 1:1, or is composed of sawdust, chaff and crop straw in a weight ratio of 5:3:2. Sawdust, chaff, and crop stalks play the roles of adsorbing bacteria, retaining moisture, loosening and ventilating, and providing C and N sources for functional microorganisms. Before making the fermentation bed, it is best to expose sawdust, rice husk, straw, etc. to the sun for 1 to 2 days, and the pens should be thoroughly disinfected.

[0071] Fermented mattress materials should have no mildew, no corruption, no foul smell or other peculiar smells. The particle size of the material is 2-10mm, the carbon-nitrogen ratio is 40-100:1, the number of fecal coliform bacteria is ≤100cfu / g, the mortality rate of roundworm eggs...

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Abstract

The invention discloses a microbial inoculum for a high-efficiency fermenting bed and a preparation method thereof. The microbial inoculum is prepared by thoroughly and evenly mixing 30-40% of Bacillus subtilis leavening powder, 30-40% of Bacillus licheniformis leavening powder and 20-30% of Candida utilis leavening powder. The microbial inoculum can effectively promote in-situ degradation of livestock and poultry excrement, obviously reduce the generation of ammonia and other putrilages in the livestock and poultry excrement, and enhance the quality of pork.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a microbial bacterial agent for a fermentation bed and a preparation method thereof. Background technique [0002] In recent years, with the rapid development of large-scale and intensive pig farming in my country, the pollution of livestock and poultry manure and sewage to the environment has become increasingly serious. According to statistics, the total amount of solid waste produced by animal husbandry in the country is about 3 billion tons every year. In Changsha City alone, about 80 million tons of livestock and poultry wastewater and more than 1.2 million tons of solid manure are discharged every year. Most farms only pass through biogas. After the pool is simply treated, it is directly discharged, which causes a lot of pollution to the environment. [0003] At present, the traditional pig manure treatment technology mostly adopts composting fermentation, biogas ferm...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C12N1/16C12R1/10C12R1/125C12R1/72
Inventor 尹红梅贺月林张德元吴迎奔陈薇许丽娟王震周映华吴胜莲
Owner HUNAN INST OF MICROBIOLOGY
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