Electricity enhanced molecular imprinting solid phase micro-extraction method

A molecular imprinting and electrical enhancement technology, applied in separation methods, chemical instruments and methods, material separation, etc., can solve the problems of inability to achieve selective extraction of target compounds, difficulty in analysis and detection of target compounds, etc., and achieve fast extraction speed and service life. The effect of long and large enrichment multiples

Inactive Publication Date: 2011-12-28
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Document prior art applies voltage on the extraction fiber of SPME, utilizes the electrophoretic migration of charged ion under the action of electric field, the ionic compound in aqueous solution is extracted (J.B.Zeng, J.M.Chen, X.H.Song, Y.R.Wang, J.H.Ha, X . Chen, X.R. Wang, J. Chromatogr. A 1217 (2010) 1735; J.B. Zeng, J. Zou, X.H. Song, J.M. Chen, J.J. Ji, B. Wang, Y.R. Wang, J.H. Ha, X. Chen, J. Chromatogr .A 1218 (2011) 191), but because the SPME stationary phase used is a non-selective material, during extraction, all ionic compounds and polar compounds in the solution are adsorbed on the SPME stationary phase under the action of an electric field, which cannot be achieved Selective extraction of target compounds, which brings great difficulties to the analysis and detection of target compounds

Method used

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  • Electricity enhanced molecular imprinting solid phase micro-extraction method
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  • Electricity enhanced molecular imprinting solid phase micro-extraction method

Examples

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Embodiment 1

[0023] Prepare 3 mg / mL of N, N-dimethylamide (DMF) carbon nanotube dispersion, insert platinum wire and stainless steel wire with a diameter of 0.6 mm into the above DMF, apply a voltage of 35.6 V, the platinum wire is used as the positive electrode, and keep it for 5 seconds A carbon nanotube layer is obtained on the surface of the platinum wire. A platinum wire with a carbon nanotube layer was inserted into the pre-polymerization solution (0.1mol / L of NaOH, 0.1mol / L of pyrrole and 0.05mol / L ofloxacin) as the positive electrode, and polymerized at a voltage of 0.95V for about 30min, A carbon nanotube-conductive ofloxacin molecularly imprinted polymer composite coating is obtained, that is, a conductive ofloxacin molecularly imprinted microextraction fiber.

[0024] Prepare 15mL 0.1μmol / L ofloxacin solution in a 20mL vial, use conductive ofloxacin molecularly imprinted micro-extraction fiber working electrode, stainless steel wire as counter electrode, calomel electrode as ref...

Embodiment 2

[0026] Prepare 3 mg / mL of N, N-dimethylamide (DMF) carbon nanotube dispersion, insert titanium wire and stainless steel wire with a diameter of 0.6 mm into the above DMF, apply a voltage of 35.6 V, and use the titanium wire as the positive electrode for 5 seconds. A carbon nanotube layer is obtained on the surface of the titanium wire. Insert a titanium wire with a carbon nanotube layer into a pre-polymerization solution (0.1mol / L NaOH, 0.1mol / L pyrrole and 0.05mol / L tetracycline) as the positive electrode, and polymerize at a voltage of 0.95V for about 30min to obtain carbon nanotubes. Tube-conductive tetracycline molecularly imprinted polymer composite coating, i.e. conductive tetracycline molecularly imprinted microextraction fibers.

[0027] Prepare 15mL of 0.1μmol / L tetracycline solution in a 20mL vial, use conductive tetracycline molecularly imprinted micro-extraction fiber working electrode, stainless steel wire as counter electrode, calomel electrode as reference elect...

Embodiment 3

[0029] Prepare 3 mg / mL of N, N-dimethylamide (DMF) carbon nanotube dispersion, insert platinum wire and stainless steel wire with a diameter of 0.6 mm into the above DMF, apply a voltage of 35.6 V, the platinum wire is used as the positive electrode, and keep it for 5 seconds A carbon nanotube layer is obtained on the surface of the platinum wire. A platinum wire with a carbon nanotube layer was inserted into the pre-polymerization solution (0.1mol / L NaOH, 0.1mol / L pyrrole and 0.05mol / L promethazine) as the positive electrode, and was polymerized at a voltage of 0.95V for about 30min to obtain Carbon nanotube-conductive promethazol molecularly imprinted polymer composite coating, that is, conductive promethazin molecularly imprinted microextraction fiber.

[0030] Prepare 15 mL of 0.1 μmol / L promethazine solution in a 20 mL vial, use the conductive promethazine molecularly imprinted micro-extraction fiber as the working electrode, the stainless steel wire as the counter electr...

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Abstract

The invention relates to an electricity enhanced molecular imprinting solid phase micro-extraction method, characterized by: using a conductive molecular imprinting micro-extraction fiber as a working electrode, a platinum wire as an electrode and a saturated calomel electrode as a reference electrode, applying a bias voltage on the working electrode, letting a target compound be subject to electrophoresis and diffusion and migration to allow the target compound to reach the surface of the working electrode to complete the molecular imprinting identification extraction; and when extracting, providing a bias voltage to the working electrode through a electrochemical work station or potentiostat. The conductive molecular imprinting micro-extraction fiber is a metal wire with the diameter of0.5-1.0 mm, and one end of the fiber has a carbon nanotube-conductive molecular imprinting polymer composite coating with the thickness of 50-100 mu m and length of 1-2 cm. The extraction method is suitable for the extraction of ionic compounds and strong polar compounds. The conductive molecular imprinting micro-extraction fiber has simple preparation, large adsorption capacity to the target compound, high extraction efficiency, and good selectivity, can be reused more than 100 times, and has wide application prospects in the field of analytical chemistry and environmental analysis.

Description

technical field [0001] The invention relates to an electrically enhanced molecular imprinting solid-phase microextraction method, which is suitable for the extraction of ionic compounds and strong polar compounds, and belongs to the technical field of sample pretreatment. Background technique [0002] Pretreatment is an important step in the analysis of complex samples. Through pretreatment, the interfering substances in the sample are removed and the target substance is concentrated to make up for the lack of existing separation technology and instrument detection sensitivity. Solid phase microextraction (SPME) is a relatively new sample preparation technique. Compared with traditional sample pretreatment techniques such as liquid-liquid extraction, Soxhlet extraction, solid phase extraction, etc., SPME has the advantages of less sample consumption, short processing time, simple operation, no secondary pollution, and easy integration with gas chromatography and liquid chro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/10G01N30/08
Inventor 谭峰刘学全燮陈景文赵洪霞
Owner DALIAN UNIV OF TECH
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