Immunochromatography detection reagent strip for combined detection of toxoplasmagondii IgG antibodies and total antibodies, and preparation method thereof

Abstract

The invention discloses an immunochromatography detection reagent strip for combined detection of toxoplasmagondii IgG antibodies and total antibodies, and a preparation method thereof, and relates to a combined detection reagent of toxoplasmagondii IgG antibodies and total antibodies. The immunochromatography detection reagent strip for combined detection of toxoplasmagondii IgG antibodies and total antibodies is characterized in that the immunochromatography detection reagent strip is provided with two reagent strip parts; each one of the two reagent strip parts comprises a carrier plate, a loading pad, a colloidal gold pad, a cellulose nitrate membrane, a contrast line and an absorption pad; and the two reagent strip parts are respectively provided with a toxoplasmagondii IgG antibody detection line and a total antibody detection line. The preparation method of the immunochromatography detection reagent strip comprises the following steps of preparing toxoplasmagondii recombinant antigens of SAG1 (P30), SAG2 (P22), ROP2 and GRA7, carrying out cellulose nitrate membrane sample application, preparing colloidal gold, labeling the prepared toxoplasmagondii recombinant antigens of SAG1 (P30), SAG2 (P22), ROP2 and GRA7 by the prepared colloidal gold, and preparing the immunochromatography detection reagent strip. The immunochromatography detection reagent strip can be utilized for detection of toxoplasmagondii IgG antibodies and total antibodies in whole blood specimens, blood serum specimens, blood plasma specimens, cerebrospinal fluid specimens and the like. The immunochromatography detection reagent strip has the advantages of very small specimen use amount, no need of special apparatuses, direct interpretation by naked eyes, simpleness, convenience, speediness, strong specificity, high sensitivity, good accuracy and reliability, low cost, and wide application.

Description

technical field [0001] The invention relates to a combined detection reagent for toxoplasma IgG antibody and total antibody, in particular to a combined detection reagent strip for toxoplasma IgG antibody and total antibody by colloidal gold immunochromatography (immunochromatography) and a preparation method thereof. Background technique [0002] Toxoplasmosis is a zoonotic parasitic disease caused by Toxoplasmagondii that seriously endangers human health. The pathogen Toxoplasma can parasitize in the nucleated cells of humans and various animals. and animals are generally susceptible. The disease is widely distributed all over the world. According to statistics, about 1 billion people in the world are infected by Toxoplasma gondii ([1] Xi Linlin, Li Wei. Research progress on pathogenic mechanism, virulence and genotype of Toxoplasma gondii[J]. Chinese Journal of Pathogenic Biology , 2009, 4(11): 859-861.). Toxoplasmosis is widely distributed in my country, and there are ...

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Problems solved by technology

[0003] The diagnosis method of toxoplasmosis involves isolating toxoplasma directly from organs, blood, cerebrospinal fluid and other tissues for etiological diagnosis, which takes days or even weeks, is time-consuming and laborious, and has little value in practical applications

Routine clinical detection mainly uses various serological methods to detect its specific IgG and IgM ([3] Zhou Bin, Zhang Jue, Wang Ke, et al. The establishment and implementation of double-labeled time-resolved fluorescence immunoassay for Toxoplasma gondii IgG and IgM antibodies Its preliminary clinical application [J]. Chinese Journal of Laboratory Medicine, 2010, 33 (010): 957-959.), serological methods can diagnose congenital, acute and chronic toxoplasmosis, but because most immunocompromised people or The anti-toxoplasma IgG titer of animals does not rise or does not appear high IgM titer, so serological methods cannot be used effectively to diagnose toxoplasmosis in immunocompromised humans or animals

In addition, the serum antibody titer will gradually decrease after the antigen disappears for a long time, so it cannot be used to evaluate the therapeutic effect

At present, there are high false positives in the detection of IgG antibodies, and the general sensitivity of IgM antibody detection is poor ([4] Han Jingyun, Liu Qian, Guo Jian. Technical progress in laboratory diagnosis of Toxoplasma gondii infection [J]. Laboratory Medicine, 2009 , 24(5): 393-395.)

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