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Plant endogenetic bacterium with heavy metal tolerance and application thereof

A technology of endogenous bacteria and heavy metals in plants, applied in the fields of agriculture and environmental pollution control, can solve the problems of long cycle, slow growth, small biomass, etc., and achieve the effects of improving stress resistance, promoting plant growth, and improving repair efficiency

Inactive Publication Date: 2012-10-17
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most hyperaccumulative plants grow slowly, have a long cycle, have small biomass, and are selective to metals, which restrict the development of phytoremediation technology.

Method used

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  • Plant endogenetic bacterium with heavy metal tolerance and application thereof
  • Plant endogenetic bacterium with heavy metal tolerance and application thereof
  • Plant endogenetic bacterium with heavy metal tolerance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The heavy metal-tolerant plant endophytic bacteria Y1-3-9 strain of the present invention is isolated and purified from Mosla chinensis leaves, and the isolation and identification method is as follows:

[0027] First clean the surface of the leaves of Elsholtzia vulgaris with tap water, then wash it with sterile water, then soak and disinfect with 75% ethanol for 5 minutes, rinse with sterile water and then immerse in 2.5% sodium hypochlorite solution for 5 minutes, and rinse with sterile water several times. Place the sterilized leaves in a mortar and grind them to homogenize. Take 0.1ml of the homogenate and spread it on an LB plate (10g peptone, 5g yeast extract, 10g NaCl, 1000mL water, 20g agar, pH 7.0), and incubate at 28°C for 3 days , Pick a single colony, streak it on the LB plate and save it after purification. The strains were inoculated into LB culture broth, shake culture at 30°C for 18h, take 1.0mL bacterial broth in Eppendorf centrifuge tube, centrifuge at 1...

Embodiment 2

[0029] Y1-3-9 (CCTCC NO: M 2011251) was streaked and inoculated into LB medium containing different heavy metal concentrations, and cultured at 30°C for 3 days to observe whether it could grow and its growth. The results are shown in Table 1. The Y1-3-9 strain can tolerate a variety of heavy metals (the minimum inhibitory concentrations of Cu, Pb, Cd, and Ni are 3.1, 4.8, 4.5, 1.7 mM, respectively).

[0030] Table 1 Growth of strains on LB medium with different concentrations of heavy metals

[0031]

[0032] In the above table, "+" means growth, and "-" means no growth.

Embodiment 3

[0034] Inoculate Y1-3-9 (CCTCC NO: M 2011251) in LB culture broth, shake culture for 20 hours, centrifuge at 4℃ to collect the bacteria, and use SM broth (glucose 1.0g, sucrose 1.0g, sodium citrate 1.0g) , Malic acid 1.0g, mannitol 1.0g, sodium acetate 1.0g, KH 2 PO 4 0.4g, K 2 HPO 4 2.0g, MgSO 4 0.2g, CaCl 2 0.1g, CuSO 4 1mg, NiSO 4 1mg, ZnSO 4 5mg, FeSO 4 5mg, MnSO 4 3mg, CoSO 4 1mg, Na 2 MoO 4 1mg, H 3 BO 3 2mg, biotin 2 (VB 6 ) 10mg, Thiamine (VB 1 ) 2mg, cyanocobalamin vitamin (VB 12 )0.1mg, pantothenic acid (VB 3 ) 5mg, folic acid 2mg, riboflavin 5mg, niacin 5mg, distilled water 1000mL, pH 6.4. Add the vitamin after filtration and sterilization. ) Wash and centrifuge twice, suspend the bacteria in SM culture medium, and inoculate SMA culture medium according to 5% of the inoculum [Add filter sterilized ACC (1-aminocyclopropane-1-carboxylic acid deaminase) to the sterile SM medium ), make the final concentration 3mM], culture for 48h under the condition of 28℃1...

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Abstract

The invention belongs to the technical field of agriculture and environmental pollution treatment, and relates to a plant endogenetic bacterium with heavy metal tolerance and an application thereof. The plant endogenetic bacterium with heavy metal tolerance is preserved in CCTCC (China Center for Type Culture Collection), the preservation date is on July 26th in 2011, the preservation number of the strain is CCTCC No.M2011251. The bacterial strain liquid preparation contains more than 1 billion / milliliter of effective viable count, the solid preparation contains more than 0.2 billion / gram of effective viable count. The strain has good activation effect on the precipitation state copper in a solution, the activation rate is more than 73%. That the plant is implanted and the bioremediation preparation is inoculated in humid soil containing heavy metals promote the absorption and the extraction of the plant to the heavy metal copper and improve the extraction and recovery efficiency.

Description

Technical field [0001] The invention belongs to the technical field of agriculture and environmental pollution control, and relates to a heavy metal-tolerant plant endophytic bacteria and its application. Background technique [0002] Research on soil heavy metal pollution and its restoration is a major issue related to the sustainable use of soil resources. Bioremediation of contaminated soil using plants focuses on pollution control and landscaping, ecological restoration and reconstruction, and sustainable development. It has received extensive attention and attention from domestic and foreign governments, universities, research institutions and commercial enterprises. Phytoremediation techniques include plant stabilization, plant volatilization and plant extraction. However, the development of phytoremediation technology relies heavily on the selection and growth of hyperaccumulator plants, and the treatment effect depends on the extraction and fixation of heavy metals by hy...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/38
Inventor 盛下放何琳燕张艳峰黄智张文辉
Owner NANJING AGRICULTURAL UNIVERSITY