Beta-1, 4-endo-chitosanase (Aus CsnA) gene cloning and preparation of recombinase

A chitosanase and gene technology, applied in the field of bioengineering, can solve problems such as no reports, and achieve the effects of large-scale industrial production, high catalytic activity and thermal stability
CN102392035AInactive Publication Date: 2012-03-28JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2012-03-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a cloning method of complete mRNA and DNA sequences of a novel endo-chitosanase gene originated from Aspergillus usamii E001 strain. The nucleotide sequences are respectively SEQ ID NO:1 and SEQ ID NO:2. It shows through bioinformatics analysis that the endo-chitosanase belongs to glucoside hydrolase family 75 and is named as Aus CsnA. The amino acid sequence is SEQ ID NO:3 and the corresponding gene is named as Aus csnA. The invention also discloses an Aus CsnA engineering bacteria construction method and a recombinant Aus CsnA highly expression and purification method. The most appropriate operation temperature and pH of the prepared recombinant Aus CsnA are respectively 50 DEG C and 5.0, and the recombinant Aus CsnA is stable below 50 DEG C when pH is 4.0-7.0. The invention has great industrial production potential and economical values.
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Description

technical field

[0001] The present invention relates to the cloning of the complete mRNA and DNA sequence of a chitosanase gene derived from Aspergillus usamii (Aspergillus usamii) E001 strain, the construction of chitosanase engineering bacteria and the preparation of recombinant chitosanase The method for high-efficiency expression and purification belongs to the technical field of bioengineering. Background technique

[0002] Chitosan (chitosan), also known as deacetylated chitin, soluble chitin, polychitosan, chitosan, polyglucosamine, etc., is a product obtained by deacetylating chitin, usually chitin When the degree of deacetylation exceeds 70%, it is called chitosan. Chitosan widely exists in shells of shrimps, crabs, insects, and cell walls of fungi and algae. The obtained chitosan oligosaccharide not only has the advantages of good water solubility and easy absorption, but also has many unique physiological activities and functional properties in recent years. Ch...

Claims

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