Preparation method for constructing collagen-based bio-macromolecular/hydroxyapatite microsphere composite bracket material

A technology of biological macromolecules and hydroxyapatite, which is applied in the field of preparing collagen-based biological macromolecules/hydroxyapatite microsphere composite scaffold materials, can solve the problems of synthesizing hydroxyapatite particles and the like, and achieves a novel preparation method. And simple, mild preparation conditions, wide range of effects

Active Publication Date: 2012-04-18
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method includes more than 10 hours of processing steps at high temperatures abov...

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  • Preparation method for constructing collagen-based bio-macromolecular/hydroxyapatite microsphere composite bracket material
  • Preparation method for constructing collagen-based bio-macromolecular/hydroxyapatite microsphere composite bracket material
  • Preparation method for constructing collagen-based bio-macromolecular/hydroxyapatite microsphere composite bracket material

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Experimental program
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Effect test

Embodiment 1

[0052] The collagen solution was prepared with hydrochloric acid of pH 3, and the collagen concentration was 8 mg / mL. Add calcium nitrate aqueous solution to the collagen solution under stirring conditions, the final concentration of calcium ions is 0.1mol / L, and then quickly mix with sodium bicarbonate aqueous solution with a concentration of 0.1mol / L, and stir for 2 minutes. The above operating temperature was maintained at 4°C. After the reaction mixture was centrifuged at low speed, it was transferred to a beaker and kept in a water bath at 25°C for 16 hours to obtain collagen / CaCO 3 Microsphere composite gel, its scanning electron microscope photo is detailed in figure 1 A; its chemical composition and phase crystal analysis are detailed in figure 1 B and figure 1 c. Collagen / CaCO 3 The microsphere composite gel was immersed in a disodium hydrogen phosphate aqueous solution with a concentration of 0.2mol / L. The temperature of the solution was controlled by a water ba...

Embodiment 2

[0054] The collagen solution was prepared with hydrochloric acid of pH 2, and the collagen concentration was 6 mg / mL. Add calcium chloride aqueous solution to the collagen solution under stirring conditions, the final concentration of calcium ions is 0.03mol / L, and then quickly mix with sodium carbonate aqueous solution with a concentration of 0.03mol / L, and stir for 0.5min. The above operating temperature was maintained at 4°C. After the reaction mixture was centrifuged at low speed, it was transferred to a beaker and kept in a water bath at 28°C for 14 hours to obtain collagen / CaCO 3 The microsphere composite gel is immersed in a disodium hydrogen phosphate aqueous solution with a concentration of 0.4mol / L, the temperature of the solution is controlled by a water bath, and the temperature is kept at 35°C for 7 days, and the phosphate aqueous solution is replaced once a day. Afterwards, the composite gel was soaked in deionized water for 5 times to remove the residual salt, ...

Embodiment 3

[0056] The collagen solution was prepared with hydrochloric acid of pH 3.5, and the collagen concentration was 3 mg / mL. Add calcium chloride aqueous solution to the collagen solution under stirring conditions, the final concentration of calcium ions is 0.06mol / L, and then quickly mix with ammonium bicarbonate aqueous solution with a concentration of 0.06mol / L, and stir for 5 minutes. The above operating temperature was maintained at 4°C. After the reaction mixture was centrifuged at low speed, it was transferred to a beaker and kept in a water bath at 25°C for 12 hours to obtain collagen / CaCO 3 The microsphere composite gel is immersed in a diammonium hydrogen phosphate aqueous solution with a concentration of 0.5mol / L. The temperature of the solution is controlled by a water bath and kept at 32°C for 2 days, and the phosphate aqueous solution is replaced every 2 days. Afterwards, the composite gel was soaked in deionized water for 5 times to remove residual salt therein, and...

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Abstract

The invention discloses a preparation method for constructing a collagen-based bio-macromolecular/hydroxyapatite microsphere composite bracket material. The preparation method is characterized in that: micron-sized CaCO3 microspheres are synthesized in the presence of bio-macromolecules, mainly collagen; the CaCO3 microspheres are taken as a template; hydroxyapatite microspheres are obtained by transforming the CaCO3 microsphere template into a calcium-phosphorus mineral in the phosphate solution environment; meanwhile, hybrid gel combined with hydroxyapatite microspheres is obtained by usinga three-dimensional fiber network formed through self-assembly of collagen macromolecules, the collagen-based bio-macromolecular/hydroxyapatite microsphere composite bracket material is further obtained, and in-situ synthesis of micron-sized hydroxyapatite microspheres in a bio-macromolecular substrate is realized under the mild condition. The calcium-phosphorus microspheres in the collagen-basedcomposite bracket material are made from carbonic acid hydroxyapatite, and have high bionics and biocompatibility on the aspect of component; and meanwhile, the composite bracket material has the nanometer-micron-macroscopic multi-scale distribution characteristic on the aspect of structure.

Description

technical field [0001] The invention relates to a preparation method for constructing a collagen-based biomacromolecule / hydroxyapatite microsphere composite scaffold material, which belongs to the fields of biological materials, tissue engineering and biomineralization. Background technique [0002] Extracellular matrix (ECM) is a complex network framework composed of biological macromolecules secreted by cells into the extracellular matrix, which supports and connects cells and tissue structures, and regulates the physiological activities of cells. Tissue engineering scaffolds are artificial bionic scaffolds with extracellular matrix functional properties constructed using specific biological materials. They provide structural support for cells that build tissues, provide a suitable physiological microenvironment for cell growth, guide tissue regeneration and repair, and control tissue morphological structure. [0003] Ideal tissue engineering scaffold materials are requir...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/24A61L27/12A61L27/50
Inventor 魏清荣
Owner SICHUAN UNIV
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