Preparation method of star-type polylactic-acid grafting sodium alginate particles
A technology of sodium alginate and polylactic acid, applied in the field of biomedical materials, can solve the problems of pure polylactic acid crystallinity weakening soft tissue compatibility, etc., and achieve good biocompatibility and degradability, good degradability, and simple method. Easy to operate effect
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Embodiment 1
[0016] Take 100mL of 0.2% sodium alginate solution, adjust the pH between 3.5-4.5, stir at room temperature for 2 hours, dissolve a certain amount of EDC in ultrapure water, add it dropwise to sodium alginate aqueous solution, and stir at room temperature for 2 hours Hour, then add sodium lauryl sulfate 0.35% (w / v), the star polylactic acid of 40mg is dissolved in 10mL methylene chloride, and is added dropwise in the aqueous solution, reacts at room temperature for 24 hours.
[0017] After the reaction, the emulsion was broken, and the dichloromethane was removed by rotary evaporation, and the pH was adjusted to neutrality, and then dialyzed with deionized water to remove EDC and SDS, and acetone was used as a solvent to remove unreacted star polylactic acid. The grafted product was dried at 40°C for 24h. The graft rate of the grafted product was 14%.
Embodiment 2
[0019] Take 100mL of 0.2% sodium alginate solution, adjust the pH between 3.5-4.5, stir at room temperature for 2 hours, dissolve a certain amount of EDC in ultrapure water, add it dropwise to sodium alginate aqueous solution, and stir at room temperature for 2 hours Hour, then add sodium lauryl sulfate 0.35% (w / v), the star polylactic acid of 60mg is dissolved in 10mL methylene chloride, and is added dropwise in the aqueous solution, reacts at room temperature for 24 hours.
[0020] After the reaction, the emulsion was broken, and the dichloromethane was removed by rotary evaporation, and then dialyzed with deionized water to remove EDC and SDS, and the acetone of the Soxhlet extractor was used as a solvent to remove unreacted star polylactic acid. The grafted product was dried at 40°C for 24h. The graft rate of the grafted product was 22%.
[0021] It can be seen from the infrared spectrum that the grafted product is at 1750cm -1 A carbonyl peak appears at the place, and i...
Embodiment 3
[0024] Preparation and in vitro release profiles of drug-loaded microspheres with different grafting ratios
[0025] Add a certain amount of bovine serum albumin (BSA) into 10mL of 2% sodium alginate solutions with different grafting ratios (ALG / BSA mass ratio 5:1), stir well, and slowly push in 50mL of 0.25mol / LCaCl 2 Into the solution, stir and add dropwise. After completion, continue to gel for 0.5h, filter, rinse with deionized water three times, and dry at low temperature to constant weight to obtain calcium alginate microspheres.
[0026] Weigh 50 mg of drug-loaded microsphere samples and place them in 200 mL of pH=6.8 (KH 2 PO 4 -NaOH), pH=1.2 (HCl solution), rotating speed 50r / min, incubating at a constant temperature of 37°C□0.5, and measuring its drug release. The release results showed that the drug release of pure sodium alginate and grafted sodium alginate in hydrochloric acid solution was about 10%, and the microspheres remained in a non-swelling state. Howe...
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