Method for preparing beta-mannase and special strain

A technology of mannanase and bacterial strain, which is applied in the field of preparation of β-mannanase, can solve the problems of low yield and achieve the effects of high yield, wide application prospect and low cost
CN102433267AInactive Publication Date: 2012-05-02INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Publication Date
2012-05-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing beta-mannase and a special strain. The strain is Pichia pastoris GS4SMAN CGMCC NO.4095. The recombinant strain disclosed by the invention contains a plurality of copied mannase genes expressed under methanol induction. Experiments show that by means of a process, disclosed by the invention, for producing alkaline mannase through high-density fermentation by using the recombinant strain, the enzyme activity of the alkaline mannase can reach 6336u / ml finally in an induction period of 84 hours. The method disclosed by the invention has the advantages of low cost, simplicity for operation and high yield. Therefore, the method disclosed by the invention has a wide application prospect in the field of mannase preparation.
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Description

technical field

[0001] The invention relates to a method for preparing β-mannanase and a special bacterial strain. Background technique

[0002] β-mannanase (β-1,4-D-mannan mannohydrolase; EC31211178) is a class of backbone β- The enzyme of 1,4-D-mannopyranose, which is used in the utilization of various β-glucosides present in some plants such as the endosperm of copra, the nuts of the ivory palm tree, the tubers of guar, acacia, coffee tree and konjac -Mannan is of potential importance. At home and abroad, it is mainly used for pulp bleaching in papermaking industry, gel breaking in oil wells, degrading plant gums to produce oligosaccharides as growth-promoting factors for bifidobacteria, health food for disease prevention and anti-aging, and anti-nutritional factors in the feed industry.

[0003] β-mannanase is divided into acidic, neutral and alkaline according to its optimal pH value, among which alkaline β-mannanase refers to the β-mannan with the highest catalytic a...

Claims

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