High-density production process for forage bacillus subtilis, microbial inoculum prepared by using forage bacillus subtilis and application of microbial inoculum

A Bacillus subtilis and production process technology, which is applied in the field of prepared bacterial agents, can solve the problems that the high-density production process of Bacillus subtilis has not yet been reported, and achieve the effects of improving animal production performance, reducing the death rate, and being convenient to use

Inactive Publication Date: 2012-05-02
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no report on the high-density production process of Bacillus subtilis ACCC10619

Method used

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  • High-density production process for forage bacillus subtilis, microbial inoculum prepared by using forage bacillus subtilis and application of microbial inoculum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1, Bacillus subtilis ACCC10619 slope activation of the present invention (substratum beef extract 0.3~0.5%, sodium chloride 0.5~1.0%, peptone 0.5~1.0%, add water to 100%; pH 6.8~7.5, place 30~34 Cultivate in a biochemical incubator for 18-24 hours), inoculate an inoculation loop into 100ml seed liquid, and carry out seed shake flask cultivation; temperature is 30°C, shaker rotation speed is 220r / min, and time is 18-24 hours.

[0029] The seed shake flask medium is 0.3% of beef extract, 0.5% of sodium chloride, 1.0% of peptone, added water to 100%, pH 7.0.

[0030] 2. The strain activated on the slant was subjected to seed shake flask culture, and then shake flask fermentation culture; the shake flask fermentation culture conditions were fermentation temperature 30° C., pH value 7.0, shaking table rotation speed 220 rpm; culture for 18 hours. The fermentation medium is composed of the following components: glucose 0.5%, starch 0.3%, bean cake powder 1.5%, 3.08% manganese s...

Embodiment 2

[0035] 1, Bacillus subtilis ACCC10619 slope activation of the present invention (substratum beef extract 0.3~0.5%, sodium chloride 0.5~1.0%, peptone 0.5~1.0%, add water to 100%; pH 6.8~7.5, place 30~34 Cultivate in a biochemical incubator for 18-24 hours), inoculate an inoculation loop into 100ml seed liquid, and carry out seed shake flask cultivation; temperature is 30°C, shaker rotation speed is 220r / min, and time is 18-24 hours.

[0036] The culture medium of the seed shake flask is beef extract 0.5%, sodium chloride 0.5%, peptone 1.0%, water is added to 100%, pH 7.0.

[0037] 2. The strain activated on the slant was subjected to seed shake flask culture, and then shake flask fermentation culture; the shake flask fermentation culture conditions were fermentation temperature 28°C, pH value 6.8, shaking table rotation speed 200rpm; culture 20h. The fermentation medium is composed of the following components: glucose 0.6%, starch 0.2%, bean cake powder 1%, 2.58% manganese sulf...

Embodiment 3

[0041] 1, Bacillus subtilis ACCC10619 slope activation of the present invention (substratum beef extract 0.3~0.5%, sodium chloride 0.5~1.0%, peptone 0.5~1.0%, add water to 100%; pH 6.8~7.5, place 30~34 Cultivate in a biochemical incubator for 18-24 hours), inoculate an inoculation loop into 100ml seed liquid, and carry out seed shake flask cultivation; temperature is 30°C, shaker rotation speed is 220r / min, and time is 18-24 hours.

[0042] The seed shake flask medium is 0.3% of beef extract, 0.5% of sodium chloride, 1.0% of peptone, added water to 100%, pH 7.0.

[0043] 2. The strain activated on the slant was subjected to seed shake flask culture, and then shake flask fermentation culture; the shake flask fermentation culture conditions were fermentation temperature 34°C, pH value 7.2, shaking table rotation speed 250rpm; culture time 18h. The fermentation medium is composed of the following components: glucose 0.75%, starch 0.4%, bean cake powder 2%, 3.88% manganese sulfate...

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Abstract

The invention relates to a high-density production process for forage bacillus subtilis, a microbial inoculum prepared by using the forage bacillus subtilis and application of the microbial inoculum. The collection number of the bacillus subtilis is ACCC10619. High-concentration bacillus subtilis powder is produced by the following steps of: performing liquid submerged fermentation culture on the bacillus subtilis, concentrating, absorbing and drying. The production process is simple, convenient to control and low in production cost; the microbial inoculum is added into feeds in the form of powder, and is convenient to store, transport and use; and the shelf life is prolonged, and the microbial inoculum has stable activity, is pollution-free, is used for pig and chicken feeds, and can improve the production performance of animals.

Description

technical field [0001] The invention relates to a high-density fermentation production process of Bacillus subtilis ACCC10619, a prepared bacterial agent and an application method of the bacterial agent. Background technique [0002] Practice has proved that with the use of antibiotic feed additives, while inhibiting pathogenic bacteria, it also inhibits the normal flora in the digestive tract of animals, destroys the micro-ecological balance of the flora, causes a large number of pathogenic bacteria to proliferate, and causes superinfection or endogenous infection. Second, the frequent and large-scale use of antibiotics causes the emergence of drug-resistant strains, increases the susceptibility of animals, causes immune dysfunction and causes gastrointestinal diseases and the resulting environmental pollution; in addition, the residues of antibiotics are also It is a great threat to human health, and the effect of its disease prevention and treatment is more and more unsat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/02A23K1/16A61K35/74A61P31/04C12R1/125A61K35/742
Inventor 周映华刘惠知吴胜莲曾艳胡新旭高书锋汪彬周小玲缪东
Owner HUNAN INST OF MICROBIOLOGY
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