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New aromatic polyketones, extraction method and application thereof

An aromatic and polyketone technology, applied in the field of microbial engineering, can solve the problems that it is difficult to meet the needs of discovering compounds with new structural characteristics or new mechanisms of action, and the compounds cannot adapt to rapid changes in the disease spectrum, and achieves low cost, short cycle and simple process. Effect

Inactive Publication Date: 2012-05-16
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, the progress in the field of pharmaceuticals in recent years has made people have higher requirements for the source and characteristics of medicinal active substances. It is also difficult to meet the needs of discovering compounds with new structural features or new mechanisms of action

Method used

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  • New aromatic polyketones, extraction method and application thereof
  • New aromatic polyketones, extraction method and application thereof
  • New aromatic polyketones, extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Insect gut fungus C. haliflora ( Daldiniaeschscholzii ) separation and purification

[0027] Dissect the broadsword mantis under sterile conditions ( Tenodera aridifolia ), the removed mantis intestinal tract was sterilized in 75% alcohol for 2 minutes, rinsed with sterile water for 3 times, then added a small amount of sterile water to grind in a sterile mortar, and the grinding solution was diluted to 10% with sterile water. -1 , 10 -2 , 10 -3 , 10 -4 0.2 ml of each gradient dilution was applied to MEA medium (20 g of malt extract, 20 g of sucrose, 1 g of peptone, 15 g of agar, 1 L of distilled water), and cultured in a 28°C incubator. After the growth of the bacteria, the hyphae at the edge of the colony were picked and purified for culture, and the insect intestinal fungus was obtained, which was identified by the Guangdong Provincial Institute of Microbiology as a carbon shell fungus ( Daldiniaeschscholzii ), is now preserved in the China Cente...

Embodiment 2

[0028] Embodiment 2: Insect gut fungus C. haliflora ( Daldiniaeschscholzii ) of liquid fermentation

[0029] Activation of the insect gut fungus C. nicheta ( Daldiniaeschscholzii ), inoculate fresh mycelium blocks into 1000 mL Erlenmeyer flasks, each containing 400 mL of ME medium, inoculate 5-6 flasks on a shaker, and cultivate them at 140 rpm and 28 °C for 3 days as seeds Then, 20 mL of seed liquid was inoculated into a 1000 mL Erlenmeyer flask containing 400 mL of ME medium, and fermented at 140 rpm and 28 °C for 10 days.

Embodiment 3

[0030] Embodiment 3: the extraction and separation of the aromatic polyketide of carbon shell fungus

[0031] The fermented liquid obtained in Example 2 was filtered through gauze, the filtrate was extracted three times with ethyl acetate, concentrated and dried in vacuo to obtain a black crude extract. Suspend the crude extract in water, first extract 3 times with petroleum ether to remove the grease part in the extract, then extract the remaining mother liquor 3 times with ethyl acetate, combine the ethyl acetate extracts and concentrate under reduced pressure to obtain 300 grams of extract, The extract was subjected to silica gel column chromatography and roughly segmented, using chloroform / methanol gradient elution, volume ratio 100:0, 100:1, 100:2, 100:4, 100:8, 100:16, 100:32 7 eluting fractions F 1 -F 7 . Continue to extract F 2 Separation by silica gel column chromatography, using chloroform / methanol system gradient elution, volume ratio 200:0, 200:1, 200:2, 200:4 ...

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Abstract

The invention belongs to the technical field of microbial engineering, and specifically relates to new daldiniaeschscholzii aromatic polyketones, an extraction method and an application thereof, wherein the aromatic polyketones are extracted from a liquid fermented material of the intestinal bacteria of Tenoderaaridifolia, and the intestinal bacteria of the Tenoderaaridifolia is the daldiniaeschscholzii. The daldiniaeschscholzii aromatic polyketones of the present invention have the following structural formula. The daldiniaeschscholzii aromatic polyketone 1 has strong antioxidant activity, and the daldiniaeschscholzii aromatic polyketones of 2-5 have strong immunosuppression activities, such that the daldiniaeschscholzii aromatic polyketones of 1-5 can be adopted for preparing the relevant novel drugs, wherein the daldiniaeschscholzii aromatic polyketones of 1-5 are adopted as the compounds with the antioxidant effects or the immunosuppression effects.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, in particular to Tenodera aridifolia ) enteric bacterium C. Daldiniaeschscholzii ) A new class of aromatic polyketides from Skeletonia skeletalum extracted from the liquid fermentation product and its preparation method and application. Background technique [0002] Since Fleming discovered penicillin from fungi in 1929, people have been trying to find new drug source molecules that are urgently needed by humans from microorganisms. The probability of new active compounds with new skeletons is very low, and the convergence environment and repeated research have made the discovery rate of new natural drugs decline year by year since the 1980s. But at the same time, the progress in the field of pharmaceuticals in recent years has made people have higher requirements for the source and characteristics of medicinal active substances. It is also difficult to meet the needs of discove...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/747C07D311/78C07C39/17C12P15/00C12P17/06A61K31/122A61K31/352A61K31/05A61P39/06A61P37/06C12R1/645
Inventor 谭仁祥徐强张应烙宋勇春张杰
Owner NANJING UNIV
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