Method for separating and purifying polysaccharide from Hovenia acerba

A technology for separation and purification of jujube, which is applied in the field of separation and purification of jujube polysaccharides, can solve problems such as the difficulty of completely removing the charcoal residue in the solution, the destruction of the polysaccharide chain structure, and the inability to guarantee the final quality, etc., and achieves easy implementation, simple preparation method, Good decolorization effect

Inactive Publication Date: 2012-05-30
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing polysaccharide decolorization technology often uses activated carbon for decolorization. Although the price is low, powdered activated carbon must be used during operation. There is a fatal shortcoming that it is difficult to completely remove the carbon residue in the solution after decolorization; hydrogen peroxide decolorization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Crush the sun-dried jujube into 80-mesh coarse powder, put it in an extraction tank, add 10 times of hot water at 90-100°C to soak for extraction, repeat the extraction 3 times, each time for 24 hours, combine the extracts, and concentrate to obtain the extract; Add 3 times the volume of 90% edible ethanol to the extract, stir for 5 minutes and let it settle, remove the supernatant by centrifugal filtration, collect the precipitate, stir and dissolve the precipitate with hot water, and put it on a DEA-23 cellulose column , 0-0.5mol / LNaCl solution gradient elution, put the pretreated Asmit 265P anion exchange resin into the column, concentrate the eluate to 0.3kg / L, put it on the column at 2BV / h, collect the next column liquid, filter and concentrate To the minimum volume, put the concentrated solution into a semi-permeable membrane dialysis bag with a molecular weight cut-off of 4000 for dialysis for 48 hours, and spray dry to obtain the jujube polysaccharide, which has ...

Embodiment 2

[0022] Crush the sun-dried jujube into 100-mesh coarse powder, put it in an extraction tank, add 14 times of hot water at 90-100°C to soak for extraction, repeat the extraction twice, each time for 48 hours, combine the extracts, and concentrate to obtain the extract; Add 4 times the volume of 85% edible ethanol to the extract, stir for 15 minutes and let it settle down, remove the supernatant by centrifugal filtration, collect the precipitate, stir and dissolve the precipitate with hot water, put it on a DEAE-52 cellulose column , 0-0.5mol / LNaCl solution gradient elution, put the pretreated Asmit 265P and Asmit295 mixed anion exchange resin into the column, concentrate the eluate to 0.44kg / L, put it on the column at 2BV / h, collect the next column liquid, After filtering and concentrating to the minimum volume, put the concentrated solution into a semi-permeable membrane dialysis bag with a molecular weight cut off of 7000 for dialysis for 24 hours, and spray dry to obtain the ...

Embodiment 3

[0024] Crush the sun-dried jujube into 80-mesh coarse powder, put it in an extraction tank, add 13 times of 90-100°C hot water for constant temperature soaking and extraction, repeat the extraction for 3 times, each time for 24 hours, combine the extracts, and concentrate to obtain the extract; Add 4 times the volume of 90% edible ethanol to the extract, stir for 10 minutes and let it settle, remove the supernatant by centrifugal filtration, collect the precipitate, stir and dissolve the precipitate with hot water, and put it on a DEAE-52 cellulose column , 0-0.5mol / L NaCl solution gradient elution, the pretreated Duolite A171P anion exchange resin was packed into the column, the eluate was concentrated to 0.38kg / L, and the column was loaded at 2BV / h, the next column liquid was collected, filtered and concentrated To the minimum volume, put the concentrated solution into a semi-permeable membrane dialysis bag with a molecular weight cut-off of 4000 for dialysis for 36 hours, fr...

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PUM

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Abstract

The present invention discloses a method for separating and purifying polysaccharide from Hovenia acerba. The method is characterized by pulverizing raw material, leaching with hot water, filtering, and concentrating to obtain an extract; adding alcohol into the extract for precipitation, and centrifuging to obtain crude polysaccharide of Hovenia acerba; and dissolving the crude polysaccharide in an appropriate amount of hot water, performing chromatography via a diethylaminoethyl (DEAE) cellulose column for removing impurity, decolorizing via an ion exchange resin column, dialyzing via a semi-permeable membrane, and drying. The prepared polysaccharide is clear white, and has little impurity and high purity. In addition, the decolorization step employs ion exchange resin, to achieve good decolorization effect and high retention rate of polysaccharide.

Description

Technical field: [0001] The invention relates to a method for separating and purifying jujube polysaccharides, belonging to the technical field of traditional Chinese medicines. Background technique: [0002] Hovenia cerba Lindl. is a deciduous tree of the Rhamnaceae genus Hovenia cerba Lindl., commonly known as dulcet, hook, chicken feet, wanziguo, longevity fruit, and dragon claw. Its whole body is full of treasures, and it is a precious tree with both fruit, medicine and materials, and has broad development prospects. The fruit contains glucose and calcium malate, and the seeds contain Hovenia dulcis saponin, which has sedative, anticonvulsant and hypotensive effects. The meat can cure rheumatism, it has the effect of clearing heat, diuresis, quenching thirst, reducing fire and detoxification, the bark can promote blood circulation, relax tendons and detoxify, the fruit stem is sweet and flat, has the effect of invigorating the stomach and blood, non-toxic, mainly treats...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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