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Fungus spore germination carrying membrane and production method thereof

A technology of spore germination and production method, which is applied in the field of bacterial spore germination carrier film and its preparation, and can solve problems such as labor-intensive, film rupture, and easy breakage

Inactive Publication Date: 2012-06-13
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Simply using agar hydrogel as the carrier film, if it is made in batches, the carrier film will be easily broken after drying, and it is inconvenient to use; if it is directly made on a small glass slide before use, it will cost materials, time and labor; 1. The thickness difference in wet state is 5~10 times (depending on the content of agar). When using wet film, it is difficult to make the thinness required for observation due to the high surface tension of agar hydrogel on the glass slide. If it is too thick, it will affect the transparency. Light; when using a dry film, it takes a long time to spread the agar hydrogel into a thin layer until it dries (it takes 24 hours or more, and the length of time varies with the temperature and humidity of the production place), so the agar hydrogel must be placed on the glass slide. If it is placed for a long time, it is troublesome to manage, and it may cause the membrane to rupture or drift into debris if it is not careful

Method used

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  • Fungus spore germination carrying membrane and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Weigh 7.0 g of potassium dihydrogen phosphate (accurate to 0.01 g) and 9.0 g of dipotassium hydrogen phosphate (accurate to 0.01 g) into a 500 mL beaker, add about 200 mL of deionized water and stir until completely dissolved; take deionized water Heat about 800 mL in a heatable container, weigh 12 g of agar (accurate to 0.1 g), pour it into hot water, and continue heating until the agar is completely dissolved; the melted agar solution is filtered with 4 layers of gauze to remove insoluble matter; Add the above-prepared inorganic salt solution to the filtrate, add 2.0 g (accurate to 0.1 g) Tween-20 and stir evenly, dilute to 1000 mL with 85°C hot water, and stir evenly; add agar solution (10 mL ~12 mL, the liquid temperature of the composition is about 60°C) and slowly pour it on a clean glass plate that has been preheated to about 60°C (60°C constant temperature water bath for about 5 minutes), spread it out, and level it into a thickness of about 1.0 mm and an area of...

Embodiment 2

[0021] Agar 10 g

[0022] Sodium dihydrogen phosphate monohydrate (NaH 2 PO 4 ·H 2 O) 17g

[0023] Disodium hydrogen phosphate dihydrate (NaH 2 PO 4 2H 2 O) 14g

[0024] Tween-20 1.5 g

[0025] Dilute to 1000mL with deionized water.

[0026] The preparation method is the same as in Example 1. The broken rate of the finished dry film is less than 2%, and the broken rate during use is less than 4%.

Embodiment 3

[0028] Agar 18 g

[0029] Potassium dihydrogen phosphate .3.5 g

[0030] Dipotassium hydrogen phosphate 4.5g

[0031] Sodium dihydrogen phosphate monohydrate (NaH 2 PO 4 ·H 2 O) 8.5g

[0032] Disodium hydrogen phosphate dihydrate (NaH 2 PO 4 2H 2 O) 7g

[0033] Tween-20 4 g

[0034] Dilute to 1000mL with deionized water.

[0035] The preparation method is the same as in Example 1. The broken rate of the finished dry film is less than 1%, and the broken rate during use is less than 5%.

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Abstract

The invention provides a fungus spore germination carrying membrane and a production method thereof. The components of the carrying membrane comprise agar, water soluble inorganic salt, Twain-20 and water, wherein the components are thermally melted and cooled, and then are spread as uniform thin layers, and solidified at a room temperature, so that the carrying membrane is obtained. The carryingmembrane produced by the method disclosed by the invention is thinner, and harder to be crushed; spore suspension can be conveniently spread as a thinner liquid layer on the carrying membrane, so that the carrying membrane is excellent in transmission of light and does not affect observation of the germination state of fungus spores; the carrying membrane is suitable for being used as a carrying member for suspensions which are prepared by using various kinds of fungus spores for germination tests; and the carrying membrane is simple in production and convenient in use. The carrying membrane produced by the method disclosed by the invention can satisfy the requirements of fungi which have high demands on oxygen for germination of spores, and realize tidy germination of the spores, is excellent in transmission of light, and does not affect observation of the germination states of the fungus spores. In addition, the carrying membrane is simple in the production method, convenient in use, easy for preservation, and the like, and the production progress and carrying membrane products are safe for operators as well as test environments.

Description

technical field [0001] The invention relates to a fungal spore germination carrier film and a preparation method thereof. It is used in the fungal spore germination test of food, grain, environmental microorganism detection, commodity inspection and quarantine, medicine, pesticide and agriculture, etc. Background technique [0002] Fungi, formerly collectively referred to as fungi, are a type of eukaryotic microorganisms closely related to human society. They can be used not only to make medicines, food, and industrial raw materials, but also to cause infectious diseases in humans, animals, and plants. The fungal spore germination test is a common technique for studying fungi. Identification of fungal species, research on strain selection, inheritance and variation, survival, reproduction and population dispersal ability, stress resistance, relationship with other organisms, research on harmful fungus control technology, etc. must be carried out Spore germination test. In...

Claims

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Application Information

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IPC IPC(8): C12M1/34C08J5/18C08L5/00C12R1/645
CPCC12M25/02
Inventor 许文耀江茂生
Owner FUJIAN AGRI & FORESTRY UNIV
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