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Method for extracting voacangine

A technology for voconine and extracting solution, which is applied in the field of extraction and separation of traditional Chinese medicine, can solve problems such as the lack of separation and purification of voconine, and achieves the effects of easy operation, high extraction rate and energy saving.

Inactive Publication Date: 2012-07-18
江苏博益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching, there is no report on the method of separating and purifying voacine

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take 1kg of African vulcan tree raw material, crush it, add pH3 sulfuric acid aqueous solution for percolation extraction, collect the strong acidic cation exchange resin on the extract, wash it with deionized water to neutrality, and then wash it with 6 times column volume of 1% sodium chloride aqueous solution For elution, the eluate is directly added to a hollow cellulose membrane system with a molecular weight cut-off of 6000 for ultrafiltration, and the permeate is added to a hollow cellulose membrane with a molecular weight cut-off of 200 to concentrate and dry to obtain a dried product.

[0018] Equip a n-hexane-ethyl acetate-methanol-water solvent system at a volume ratio of 3:2:5:1, place the system in a separatory funnel and mix thoroughly, separate the upper phase and the lower phase, and remove air bubbles by ultrasonic treatment , pump the upper phase (stationary phase) into the chromatographic column, turn on the machine after the column is filled with the ...

Embodiment 2

[0020] Take 1kg of the raw material of African vulcan tree, crush it, add pH 1 aqueous hydrochloric acid solution for percolation extraction, collect the strong acidic cation exchange resin on the extract, wash it with deionized water to neutrality, and then use 4 times the column volume of 4% sodium chloride aqueous solution For elution, the eluate is directly added to a tubular cellulose membrane system with a molecular weight cut-off of 2000 for ultrafiltration, and the permeate is added to a tubular cellulose membrane with a molecular weight cut-off of 200 to concentrate and dry to obtain a dried product.

[0021] Equip n-hexane-ethyl acetate-methanol-water solvent system with a volume ratio of 6:7:8:3, place the system in a separatory funnel and mix thoroughly, separate the upper and lower phases, and remove bubbles by ultrasonic treatment , pump the upper phase (stationary phase) into the chromatographic column, turn on the machine after the column is filled with the stat...

Embodiment 3

[0023] Take 10kg of the raw material of African vulcan tree, pulverize it, add pH2 sulfuric acid aqueous solution to percolate and extract, collect the strong acidic cation exchange resin on the extract, wash it with deionized water to neutrality, and then use 5 times the column volume of 3% sodium chloride aqueous solution For elution, the eluate is directly added to a tubular cellulose membrane system with a molecular weight cut-off of 2000 for ultrafiltration, and the permeate is added to a hollow cellulose membrane with a molecular weight cut-off of 200 to concentrate and dry to obtain a dried product.

[0024] Equip a n-hexane-ethyl acetate-methanol-water solvent system at a volume ratio of 5:4:6:2, place the system in a separatory funnel and mix thoroughly, separate the upper and lower phases, and remove bubbles by ultrasonic treatment , pump the upper phase (stationary phase) into the chromatographic column, turn on the machine after the column is filled with the station...

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PUM

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Abstract

The invention discloses a method for extracting voacangine, which is characterized in that steps of solvent extraction, ion resin adsorption, film concentration and high-speed counter-current chromatography purification are adopted for obtaining the voacangine of which the content being more than 98%. The method is simple and convenient, easy to implement and low in cost; and the product purity is high.

Description

Technical field: [0001] The invention belongs to the technical field of extraction and separation of traditional Chinese medicines, and in particular relates to a method for extracting voracine. Background technique: [0002] Voacangine (Voacangine), is a kind of indole alkaloid isolated from the African Voacang tree, modern research it has a variety of pharmacological effects: (1) excitatory central nervous system, in the addiction test, its SDD125 (Standard delying dose 125) is 0.0212 μM / kg; (2) analgesic effect, has inhibitory effect on HOAc-induced writhing in mice, and at 50 mg / kg, the inhibition rate> 90%; (3) cooling effect, oral administration of 50 mg in mice / kg, the body temperature dropped by 1.8°C; (4) guinea pig test had local anesthetic effect; (5) sea shrimp test, LC50 was 6ppm. [0003] Vauconine is prismatic needle crystal (ethanol), mp.136~137℃, sublimation temperature 135℃ / 0.01mmHg, [α]20 / D-42°(c=1.26, chloroform). Soluble in acetone, chloroform, sli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D471/22
Inventor 陈洪生程志鹏
Owner 江苏博益生物科技有限公司