Method for preparing neutral bleaching xylanase

A xylanase and neutral technology, which is applied in the field of preparation of neutral bleaching xylanase, can solve the problems of level dyeing, low dyeing defective rate, low bulkiness of cottonseed hulls and low whiteness. Increase insignificant problems such as saving dyes, reducing the rate of defective dyeing, and improving hand and softness

Inactive Publication Date: 2012-07-18
SHANGHAI DAIDI INDAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, how to apply xylanase in the field of textile biorefining and bleaching, and solve the problem of low bulkiness of cottonseed hulls, insignificant increase in whiteness, easy damage to fibers, affecting fiber strength, level dyeing, color yield, and dyeing Problems such as low defective rate and long process time have become an important research topic

Method used

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  • Method for preparing neutral bleaching xylanase
  • Method for preparing neutral bleaching xylanase

Examples

Experimental program
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Effect test

Embodiment 1

[0026] 1. Bacteria culture on inclined plane

[0027] Pichia pastoris is used as the production strain, and the BMGY slant medium used includes: 1% yeast powder, 2% peptone, 1% glycerol, 1.34% YNB, adjust the pH to 6.0, then add 2.5% agar, and culture at 30°C For 48 hours, store in a 4°C refrigerator.

[0028] 2. Shake flask scale-up culture

[0029] Pick a single colony and inoculate it into a Erlenmeyer flask containing 500ml of BMGY, cultivate overnight at 30°C and 250r / min, and when the OD reaches 2-6, add the culture into the tank.

[0030] 3. Amplified cultivation of primary seed tanks

[0031] According to the ingredients of the first-level seed tank formula, trace elements such as magnesium, iron, and magnesium sulfate are added after feeding. After inoculation to the first-level seed tank for amplified cultivation, the tank pressure is 0.07Mpa, and the air volume is 16m 3 / h, without stirring, with a natural pH value, and cultured at 29°C for 15 hours. 0 hours af...

Embodiment 2

[0040] 1. Bacteria culture on inclined plane

[0041] Pichia pastoris is used as the production strain, and the BMGY slant medium used includes: 1% yeast powder, 2% peptone, 1% glycerol, 1.34% YNB, adjust the pH to 6.0, then add 2.5% agar, and culture at 30°C For 48 hours, store in a 4°C refrigerator.

[0042] 2. Shake flask scale-up culture

[0043] Pick a single colony and inoculate it into a Erlenmeyer flask containing 500ml of BMGY, cultivate overnight at 30°C and 250r / min, and when the OD reaches 2-6, add the culture into the tank.

[0044] 3. Amplified cultivation of primary seed tanks

[0045] According to the ingredients of the first-level seed tank formula, trace elements such as magnesium, iron, and magnesium sulfate are added after feeding, and the raw materials in the tank are fixed to 150L. The actual consumption adopts direct air intake to ensure that the volume after the actual consumption is 180L, and the pH is 5.0. After disinfection, inoculate to the first...

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Abstract

The invention provides a method for preparing neutral bleaching xylanase, which comprises the following steps of: taking pichia pastoris as production strains, adopting a test-tube slant for activating, shake-flask amplification culturing, amplification culturing in a primary seeding tank, amplification culturing in a secondary seeding tank, and fermentation culturing in a fermentation tank. Due to the adoption of the method for preparing the neutral bleaching xylanase, the blank of application of the xylanase to a textile dyeing and bleaching process is filled, the filling power of cottonseed hulls can be enhanced so as to enable the removing effect of the cottonseed hulls to be good, the whiteness is obviously increased, the fiber cannot be damaged, the fiber strength cannot be affected, the weight loss of textile is reduced, the levelling property is good, the color yield is improved, the dyeing defective rate is reduced greatly, the dye can be saved, the hand feel and the softness can be improved simultaneously, the cloth cover is more bright, the textures are clearer, the processing time is shortened, and the production efficiency is improved.

Description

technical field [0001] The invention relates to a method for preparing a bleaching product, in particular to a method for preparing a neutral bleaching xylanase. Background technique [0002] At present, the alkali oxidation method (2g / L alkali and 3-5g / L hydrogen peroxide) is mainly used in the pretreatment method of printing and dyeing for refining and bleaching, which not only damages the fiber, but also causes serious harm to the environment, increasing the burden of sewage treatment and energy consumption. Neutral bleaching xylanase-assisted bleaching technology is a brand-new mild green bleaching method, which can be used together with neutral partial alkaline pectinase to maintain the strength of the fiber to the greatest extent, with little weight loss, and the fabric feels soft and thick, and Resilient and most importantly bleaches well. [0003] Xylan is the main hemicellulose component in plant cells, accounting for 35% of the dry weight of plant cells. It is a r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42D06L3/11D06L3/00C12R1/84D06L4/40
Inventor 骆新俊赵彰
Owner SHANGHAI DAIDI INDAL DEV
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