Porous biosensor and making and application methods
A biosensor and pore-shaped technology, applied in the field of biosensors, can solve problems such as poor stability, decreased sensitivity of biosensors, and small linear range, and achieve high sensitivity and stability, enhanced electron transfer capabilities, and large linear range of determination. Effect
Inactive Publication Date: 2014-04-30
厦门思明银河整形外科门诊部有限公司
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Problems solved by technology
Biosensors based on zinc oxide nanomaterials show high sensitivity and rapid response, but the linear range of determination is often small, which limits its application range. For example, the linear range of glucose sensors based on zinc oxide nanowires is 0.01-3.45 mM, but the normal human blood sugar level is between 3.5 and 6.1mM
Moreover, with the passage of time, the activity of biomolecules adsorbed on the surface of the zinc oxide electrode will decrease, resulting in a decrease in the sensitivity and poor stability of the biosensor.
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[0028] The present invention will be further described below in conjunction with accompanying drawing:
[0029] The first is the fabrication method of the porous biosensor.
[0030] refer to figure 1 , making a porous biosensor mainly includes the following steps
[0031] Step 1: Fabrication of substrate electrodes. Electrode materials can be made of precious metals, graphite and other materials. Taking the production of noble metal electrodes as an example, firstly, a layer of noble metal is deposited on the surface of the glass substrate by evaporation coating equipment, and then the electrode array pattern is etched on the glass substrate by photolithography technology. connected to the voltammetry analysis circuit of the instrument.
[0032] Step 2: Fabrication of an array of wells in the polymer film layer well-shaped structure area. Attach a layer of polydimethylsiloxane (PDMS) film with a thickness of 1 mm (other thicknesses can be selected according to needs) on t...
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Abstract
The invention discloses a porous biosensor and making and application methods. The porous biosensor is characterized by sequentially comprising a substrate, an electrode layer and a polymer film layer. A polymer film in a substrate electrode sensing region is removed, and the exposed substrate electrode region and the edge of the polymer film layer form a well-like structural region array; a zinc oxide coating with a porous structure is filled in each well-like structural region; and bioactive substance molecules with negative electricity are adsorbed in a porous zinc oxide structure under the action of static electricity, and a nano-scale narrow bottleneck structure is adopted between inner holes of each coating with the porous structure.
Description
technical field [0001] The invention relates to a biosensor, in particular to a biosensor with a continuous hole structure, a manufacturing method of the biosensor and a detection method applied to biomedicine. Background technique [0002] Biosensors can selectively detect analytes by using the affinity of biologically active substances, such as molecular recognition functions of enzyme-substrate, enzyme-prosthetic group, antigen-antibody, hormone-receptor, etc. Due to the specific recognition function of biologically active substances, biosensors have high selectivity and can be directly used for the detection of complex samples. Biosensors have been widely used in many fields such as clinical medical testing, industrial process control, environmental testing, chemical substance safety evaluation, food, and pharmaceuticals. [0003] In the construction of biosensors, one of the key technologies is how to immobilize biomolecules on the transducer surface stably and with hi...
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IPC IPC(8): G01N27/327
Inventor 游学秋卢家宾王晓诚
Owner 厦门思明银河整形外科门诊部有限公司