High-efficient in-vitro propagation method of 20-year-old schima superba big tree
A technology for in vitro propagation and large trees, applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems of easy browning and vitrification of seedlings, low rooting rate, low multiplication coefficient, etc., and achieve effective Controlled production and browning, high rooting rate, fast start-up effect
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Embodiment 1
[0050] The formula of the basic medium used in the high-efficiency in vitro propagation method of the twenty-year-old Schima superba is shown in Table 2
[0051] Table 2 The type and quality of substances contained in each liter of basic medium
[0052] Chemical Name
Chinese name
mg / L
Ca(NO 3 ) 2 4H 2 o
calcium nitrate tetrahydrate
371
NH 4 NO 3
ammonium nitrate
268
KNO 3
potassium sulfate
600
MgSO 4 ·7H 2 o
Magnesium Sulfate Heptahydrate
248
K H 2 PO 4
Potassium dihydrogen phosphate
115
CaCl 2 2H 2 o
Calcium Chloride Dihydrate
70
MnSO 4 4H 2 o
Manganese Sulfate Tetrahydrate
22.5
ZnSO 4 ·7H 2 o
Zinc sulfate heptahydrate
8.6
h 3 BO 3
boric acid
6.2
CuSO 4 ·5H 2 o
Copper Sulfate Pentahydrate
0.25
Na 2 MoO 4 2H 2 o
Sodium molybdate dihydrat...
Embodiment 2
[0066] This embodiment is operated according to the steps of Example 1, except that the weight ratio of the medium and the raw material components are different. The formula of the basic medium used in this example is shown in Table 3, and the rooting rate after cultivation is 96.8%.
[0067] Table 3 The type and quality of substances contained in each liter of basic medium
[0068] Chemical Name
Chinese name
mg / L
Ca(NO 3 ) 2 4H 2 o
calcium nitrate tetrahydrate
556
NH 4 NO 3
ammonium nitrate
400
KNO 3
potassium sulfate
900
MgSO 4 ·7H 2 o
Magnesium Sulfate Heptahydrate
370
K H 2 PO 4
Potassium dihydrogen phosphate
170
CaCl 2 2H 2 o
Calcium Chloride Dihydrate
96
MnSO 4 4H 2 o
Manganese Sulfate Tetrahydrate
22.5
ZnSO 4 ·7H 2 o
Zinc sulfate heptahydrate
8.6
h 3 BO 3
boric acid
6...
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