High-efficient in-vitro propagation method of 20-year-old schima superba big tree

Abstract

The invention relates to a high-efficient in-vitro propagation method of a 20-year-old schima superba big tree. The high-efficient in-vitro propagation method comprises the following steps of: taking a stem segment with axillary buds, which is grown on the 20-year-old schima superba big tree, as an explant, performing disinfection and sterilization, then inoculating into a starting culture medium, firstly placing under dark conditions for culturing for 7-12 days, further placing under an ordinary fluorescent lamp, performing illumination for 16h daily, culturing for 40 days under conditions that the temperature is 23-25 DEG C and the humidity is 50%-60%, and performing differentiation to grow into a test tube seedling; and shearing the grown test tube seedling, transferring into a propagation culture medium after high-temperature sterilization for propagation culture, then shearing the test tube seedling after propagation, then inoculating into a seedling strengthening culture medium after high-temperature sterilization for seedling strengthening culture, exterminating calli and part of the leaf blades after 32 days, retaining 3-4 blades, inoculating into a rooting culture medium after high-temperature sterilization for performing rooting culture for 12-15 days, taking out, cleaning, transplanting into a mixed matrix containing turf, yellow soil and perlite, then rooting and surviving after 25 days, wherein the volume ratio of the turf to the yellow soil to the perlite is 2: 1: 1, and the rooting rate is above 96%.

Description

technical field [0001] The invention relates to a method for fast propagation of plant in vitro tissue culture, in particular to a method for high-efficiency in vitro tissue culture and rapid propagation of a 20-year-old Schima superba. Background technique [0002] Schima superba (Schima superba) belongs to the genus Schima superba of the family Theaceae. It is a large evergreen tree in the subtropical zone. It is widely distributed in 12 southern provinces as a characteristic tree species for fast-growing landscape and fire prevention. Schima superba grows rapidly, has few pests and diseases, does not have strict requirements on soil conditions, and has strong drought tolerance. At the same time, it has a dense crown, thick leathery leaves, high water content, low oil content, high ignition point, and strong germination. It is a biological fire prevention forest belt in the south. The main tree species constructed and excellent high-resistance ecological protection tree sp...

AI Technical Summary

Problems solved by technology

The reason is that when the original method was used to cultivate, it was found that the explants were difficult to start culture, the proliferation coefficient was low, browning and vitrified seedlings were prone to occur, and the rooting rate was low.