New application of stilbene glucoside to preparation of skin ageing-resistant medicaments
A stilbene glycoside, anti-skin aging technology, applied in the field of medicine, can solve the problems of single action, cure the symptoms but not the root cause, achieve the effect of scavenging oxygen free radicals, overcome the single action, and have wide application prospects
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Embodiment 1 2
[0007] Example 1. Test of the protective effect of stilbene glycosides on the skin of photoaging mice:
[0008] Kunming mice, male, weighing 20-30 g, had free access to food and water. At the beginning of the experiment, the mice were randomly divided into blank control group, model control group and stilbene glycoside dose groups. The back skin of all mice was prepared 1×2 cm twice a week. Solar simulator 15cm distance irradiation 50mJ / cm 2 , once every other day, for 4 weeks. 30 minutes after the end of each irradiation, the blank and model control groups were given 1ml / kg of distilled water, and the doses of stilbene glycosides were 40mg / kg, 80mg / kg and 160mg / kg respectively, and all drugs were applied; the last administration After 1 h, the samples were taken, the skin tissue was homogenized, the activity of antioxidant enzyme SOD in the mouse skin was detected by the xanthine oxidase method, and the GSH-px activity was measured by the kit method.
[0009] Table 1 SOD ...
Embodiment 2
[0013] Example 2. Test of the protective effect of stilbene glycosides on the skin of drug-induced aging mice:
[0014] Kunming male mice, weighing 50-60 g, were given free access to water and food. Mice were randomly divided into blank control group, model control group and stilbene glycoside dose groups. The blank control group and the model control group were given 1ml / kg of normal saline, and the dosage groups of stilbene glycosides were 21mg / kg, 42mg / kg and 84mg / kg respectively. All the drugs were given by intragastric administration once a day. After 15 minutes of drug administration, except the blank control group, the mice in other groups were subcutaneously injected with D-galactose 150 mg / kg / d in the neck, and the blank control group was injected with normal saline. Continuously injected for 60 days, started administration from the 30th day according to the above scheme, 24 hours after the last administration, the mice were killed by neck dislocation, the skin was p...
Embodiment 3 2
[0019] Example 3 Experiment of stilbene glycosides on ultraviolet-induced human keratinocyte damage repair
[0020] Human keratinocytes (HaCaT) were randomly divided into five groups according to the exponential growth phase, blank control group, model group, and treatment group (0.001mmol / L, 0.01mmol / L, 0.1mmol / L), 12 hours before UVA radiation Add drug to incubate, rinse with PBS three times before radiation, UVA radiation, intensity 18mJ / cm 2 , and then continue to culture with drug-containing medium for 12 hours. Trypsin digestion, 1000r / min, centrifuge for 5min, discard the supernatant, adjust the cell concentration to 2.0×10 with double distilled water 10 / L, the kit detects the activities of SOD and GSH-px.
[0021] Table 3 Effect of stilbene glycosides on SOD activity in UV-induced human keratinocyte injury
[0022]
[0023] a Pb P<0.01 compared with blank control group
[0024] Keratinocytes are the most important cells that make up the epidermis. Most of th...
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