Feed additive for improving output and quality of silkworm cocoons and preparation method thereof
A technology of feed additive and yield, which is applied in the field of silkworm feed additive, can solve the problems of not being popularized and applied, complex operation, high cost of equipment and equipment, and achieve significant economic and social benefits, broad market prospects, and stable product properties
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Embodiment 1
[0020] Embodiment 1, a kind of preparation method of the feed additive that improves cocoon production and quality of silkworm, comprises the following steps:
[0021] 1. Acquisition of anti-radiation bacteria strains
[0022] The radiopugnance strain D. radiopugnance with the preservation number ATCC No. 19172 was provided by the American Type Culture Collection.
[0023] 2. Activation of bacteria
[0024] Streak inoculate the above-mentioned radiation-resistant bacteria on TGY solid medium, culture at 30°C for 36 hours, pick a single colony and inoculate it in the TGY liquid culture in the Erlenmeyer flask based on 30°C cultivation to the logarithmic growth phase; obtain the activated seed liquid .
[0025] TGY liquid medium preparation: every 1000mL of TGY liquid medium consists of 6g of peptone, 3g of yeast extract, 1g of glucose, and distilled water to 1000mL; then perform conventional high-temperature sterilization (1.1 atmospheres, 121°C for 20min).
[0026] TGY soli...
Embodiment 2
[0063] Embodiment 2, a kind of preparation method of the feed additive that improves cocoon production and quality of silkworm, comprises the following steps:
[0064] 1. Acquisition of anti-radiation bacteria strains
[0065] With embodiment 1.
[0066] 2. Activation of bacteria
[0067] Streak inoculate the above-mentioned radiation-resistant bacteria on TGY solid medium, culture at 32°C for 34 hours, pick a single colony and inoculate it in the TGY liquid culture in the Erlenmeyer flask based on 32°C cultivation to the logarithmic growth phase; obtain the activated seed liquid .
[0068] The preparation of TGY liquid medium and the preparation of TGY solid medium are all the same as in Example 1.
[0069] 3. Fermentation culture
[0070] The seed bacterium liquid after the activation of step 2 gained is that the inoculum amount of 6% is inserted in the substratum (defatted soybean substratum) that is prepared with defatted soybean by volume ratio and carries out ferment...
Embodiment 3
[0080] Embodiment 3, a kind of preparation method of the feed additive that improves cocoon production and quality of silkworm, comprises the following steps:
[0081] 1. Acquisition of anti-radiation bacteria strains
[0082] With embodiment 1.
[0083] 2. Activation of bacteria
[0084] Streak-inoculate the above-mentioned radiation-resistant bacteria on TGY solid medium, culture at 28°C for 38 hours, pick a single colony and inoculate it in the TGY liquid culture in the Erlenmeyer flask based on 28°C cultivation to the logarithmic growth phase; obtain the activated seed liquid .
[0085] The preparation of TGY liquid medium and the preparation of TGY solid medium are all the same as in Example 1.
[0086] 3. Fermentation culture
[0087] The activated seed bacterial liquid obtained in step 2 is inserted into the defatted soybean medium with an inoculation amount of 4.5% by volume to carry out fermentation culture, and cultivated at 32° C. for 65 hours to obtain a fermen...
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Abstract
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