Feed additive for improving output and quality of silkworm cocoons and preparation method thereof

A technology of feed additive and yield, which is applied in the field of silkworm feed additive, can solve the problems of not being popularized and applied, complex operation, high cost of equipment and equipment, and achieve significant economic and social benefits, broad market prospects, and stable product properties

Inactive Publication Date: 2012-08-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned method has the following disadvantages at the same time: when the silkworm eggs or larvae are processed by physical methods such as high-voltage electrostatic field treatment and radiation irradiation, the cost of equipment used is relatively high; sugar, trace elements, magnetically treated water, and triacontanol are added. , urea, etc. have single or similar components, th

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0020] Example 1. A method for preparing a feed additive for improving the yield and quality of cocoons produced by silkworms includes the following steps:

[0021] 1. Obtaining radiation-resistant strains

[0022] The strain D.radiopugnance, which has a deposit number of ATCC No.19172, which is resistant to radiation bacteria, was provided by the American Culture Collection.

[0023] 2. Activation of strains

[0024] Streak the above-mentioned radiation resistant bacteria on TGY solid medium, culture at 30°C for 36 hours, pick a single colony and inoculate the TGY liquid medium in a triangular flask at 30°C and cultivate to logarithmic growth phase; obtain the activated seed bacterial solution .

[0025] TGY liquid medium preparation: Each 1000mL TGY liquid medium consists of 6g peptone, 3g yeast extract, 1g glucose, and distilled water to 1000mL; then perform conventional high temperature sterilization (1.1 atmospheres, sterilization at 121°C for 20 min).

[0026] TGY solid medium pre...

Example Embodiment

[0063] Example 2. A method for preparing a feed additive for improving the yield and quality of cocoons produced by silkworms includes the following steps:

[0064] 1. Obtaining radiation-resistant strains

[0065] The same as in Example 1.

[0066] 2. Activation of strains

[0067] Streak the above-mentioned radiation resistant bacteria on TGY solid medium, culture at 32°C for 34 hours, pick a single colony and inoculate the TGY liquid medium in a triangular flask at 32°C and cultivate it to the logarithmic growth phase; obtain the activated seed bacterial solution .

[0068] The preparation of the TGY liquid medium and the preparation of the TGY solid medium are the same as in Example 1.

[0069] 3. Fermentation culture

[0070] The activated seed bacterial liquid obtained in step 2 was inserted into a medium prepared with defatted soybeans (defatted soybean medium) at an inoculum of 6% by volume for fermentation culture, and cultured at 32°C for 65 hours to obtain a fermentation broth...

Example Embodiment

[0080] Embodiment 3. A preparation method of a feed additive for improving the yield and quality of silkworm cocoons, including the following steps:

[0081] 1. Obtaining radiation-resistant strains

[0082] The same as in Example 1.

[0083] 2. Activation of strains

[0084] Streak the above-mentioned radiation-resistant bacteria on TGY solid medium, culture at 28°C for 38 hours, pick a single colony and inoculate the TGY liquid medium in the Erlenmeyer flask and cultivate it to logarithmic growth phase at 28°C; obtain the activated seed bacterial solution .

[0085] The preparation of the TGY liquid medium and the preparation of the TGY solid medium are the same as in Example 1.

[0086] 3. Fermentation culture

[0087] The activated seed bacterial solution obtained in step 2 was put into a defatted soybean medium at an inoculum of 4.5% by volume for fermentation and culture, and cultured at 32° C. for 65 hours to obtain a fermentation broth (cell fermentation broth). The preparation ...

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Abstract

The invention discloses a preparation method for a feed additive for improving output and quality of silkworm cocoons, which comprises the following steps: inoculating strains of D. radiopugnance with the preservation number of ATCC 19172 on a solid medium to be cultivated, and then picking single bacterial colony to be inoculated on a liquid medium to obtain activated seed bacterium liquid; inoculating the activated seed bacterium liquid on a fermentation medium according to the inoculation amount of 4.5% to 6.0%, and cultivating for 60-72 hours at the temperature of 28 DEG C to 32 DEG C to obtain fermentation liquid; and centrifuging the fermentation liquid, laying obtained thallus of the D. radiopugnance to enable the thickness to be from 0.1cm to 1.0cm, then processing the obtained thallus of the D. radiopugnance for 40-60s with the frequency of 600-700MHz and the electric-field strength of 8,000-9,000 V/cm, drying with cold air until the water content is smaller than 8% and obtaining the feed additive for improving output and quality of silkworm cocoons.

Description

technical field [0001] The invention belongs to the technical field of silkworm feed additives, and relates to a feed additive for improving the output and quality of silkworm cocoons and a preparation method thereof. Background technique [0002] Sericulture production is an industry with quick results and large profits, and it is the pillar of rural economic development and an important means for farmers to get rid of poverty and become rich; sericulture is also a traditional export industry that earns foreign exchange, and its export volume ranks first in the world. Although since the 1950s, through the replacement of silkworm varieties, the production and quality of silkworm cocoons have achieved certain development, but it is still far from meeting the development needs of the current era, improving the production performance of silkworms, and increasing the production of silkworm cocoons. Quality is one of the goals that people in the silk industry always pursue. In o...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16C12N1/20C12R1/01
Inventor 屠振力
Owner ZHEJIANG UNIV
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