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Application of combination of tetracycline medicine and fluconazole in preparation of antifungal product, and product thereof

A fluconazole and antifungal technology, applied in the field of medicine, can solve problems such as difficulty in clinical treatment of fungal infections, and achieve strong effects

Inactive Publication Date: 2014-08-06
QIANFOSHAN HOSPITAL OF SHANDONG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The formation of BF is one of the important mechanisms of clinical drug resistance, and the clinical treatment of BF-associated fungal infections is very difficult

Method used

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  • Application of combination of tetracycline medicine and fluconazole in preparation of antifungal product, and product thereof
  • Application of combination of tetracycline medicine and fluconazole in preparation of antifungal product, and product thereof
  • Application of combination of tetracycline medicine and fluconazole in preparation of antifungal product, and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 FLC and DOX / MINO combined antifungal action assay

[0039] 1. Materials

[0040] 1.1 Drugs and reagents

[0041] Fluconazole (FLC), Shandong Chengchuang Pharmaceutical Technology Development Co., Ltd.;

[0042] Minocycline (minocycline, MINO), minocycline hydrochloride, Shandong Panxin Biotechnology Co., Ltd.;

[0043] Doxycycline (DOX), Shandong Drug Testing Institute;

[0044] CHROMagar Candida Chromogenic Medium, Zhengzhou Bosai Biological Engineering Co., Ltd.;

[0045] TTC-Sapauer agar, Hangzhou Tianhe Microbiological Reagent Co., Ltd.;

[0046] Sodium hydroxide, state-owned Shandong Shanxian Organic Chemical Factory, batch number 940420;

[0047] Potassium dihydrogen phosphate, Shanghai Xinbao Fine Chemical Factory, batch number 200602132.

[0048] Dimethyl sulfoxide (DMSO), China Yaoshun Import and Export Co., Ltd., batch number 031012;

[0049] RPMI 1640 API powder, American GIBCO company;

[0050] 3-(N-morpholino)propanesulfonic acid (MOPS)...

Embodiment 2

[0085] The influence of embodiment 2 calcium regulators on FLC and DOX / MINO antifungal action

[0086] 1. Materials

[0087] 1.1. Drugs and reagents

[0088] Benidipine (benidipine hydrochloride, BEN), Huaxia Pharmaceutical Co., Ltd.;

[0089] EDTA, No.1 Reagent Factory, Shanghai, China;

[0090] Nifedipine (Nifedipine, NIF), Shandong Provincial Drug Testing Institute;

[0091] Doxycycline (DOX), National Institute of Drug Control;

[0092] Drug stock solution: all the following drug solutions are stored in a -20°C refrigerator for later use.

[0093] Benidipine was made into 2560μg / ml stock solution in DMSO

[0094] Minocycline was made into 6400μg / ml stock solution in DMSO

[0095] Fluconazole was made into 2560μg / ml stock solution with sterile distilled water

[0096] Doxycycline was made into 6400 μg / ml stock solution with sterile distilled water

[0097] Nifedipine was made into 6400 μg / ml stock solution with sterile distilled water

[0098] EDTA was made into 0....

Embodiment 3

[0120] Quantitative Determination of Example 3 Calcium Regulators to the Synergistic Effect of Combined Drugs

[0121] 1. Materials

[0122] 1.1 Drugs and reagents

[0123] Benidipine (benidipine hydrochloride, BEN), Huaxia Pharmaceutical Co., Ltd.;

[0124] EDTA, No.1 Reagent Factory, Shanghai, China;

[0125] Nifedipine (Nifedipine, NIF), a gift from the Pharmacology Laboratory of Huazhong University of Science and Technology;

[0126] Doxycycline (DOX), National Institute of Drug Control;

[0127] Drug stock solution: all the following drug solutions are stored in a -20°C refrigerator for later use.

[0128] Benidipine was made into 2560μg / ml stock solution in DMSO

[0129] Minocycline was made into 6400μg / ml stock solution in DMSO

[0130] Fluconazole was made into 2560μg / ml stock solution with sterile distilled water

[0131] Doxycycline was made into 6400 μg / ml stock solution with sterile distilled water

[0132] Nifedipine was made into 6400 μg / ml stock solution...

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Abstract

The invention discloses the application of fluconazole combined with tetracycline drugs in the preparation of antifungal products and the product thereof. The combined application of fluconazole, tetracycline antibacterial drugs doxycycline and minocycline can produce synergistic antifungal function, and three cellular calcium regulators: non-selective calcium channel blocker benidipine, selective L-type calcium channel blocker nifedipine and calcium ion chelator ethylenediaminetetraacetic acid (EDTA) The synergistic effect of azole and the combination of the above two drugs and its application in anti-Candida albicans. The results confirmed that the combination of FLC and DOX / MINO can produce a synergistic antifungal effect, especially against drug-resistant Candida albicans; BEN, NIF and EDTA can significantly enhance the activity of FLC and DOX / MINO against Candida albicans. The combined antifungal effect can significantly reduce the minimum effective concentration of FLC, MINO and DOX when combined medication; and the combination of the three calcium regulators and fluconazole can also enhance their antifungal effect.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to the application of tetracycline drugs doxycycline and minocycline combined with fluconazole in the preparation of antifungal products, and further relates to calcium channel blockers benidipine, nifedipine and calcium channel blockers Combined antifungal effects of ion chelator EDTA combined with fluclozole and fluconazole and doxycycline / minocycline against Candida albicans with different sensitivities. The present invention also relates to products containing the above components or contents. Background technique [0002] In recent years, with the increase in the incidence of malignant tumors, AIDS, and autoimmune diseases, the development of techniques such as catheter intubation and organ transplantation, and the widespread use of broad-spectrum antibiotics, corticosteroids, and immunosuppressants, the incidence of fungal infections has increased sharply. Rising (Odds FC. P...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/65A61K31/4196A61P31/10A61K31/4545A61K31/4422A61K31/198
Inventor 孙淑娟刘萍高媛
Owner QIANFOSHAN HOSPITAL OF SHANDONG
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