Method for preparing complex organ precursor with branch vessel network

Abstract

The invention provides a method for preparing complex organ precursor with a branch vessel network. The method comprises the following steps: firstly preparing one or more cellular matrix solutions and a synthetic macromolecular solution; pouring the cellular matrix solution into a combined mold in layers for physical or chemical cross-linking to form a multilayered cellular matrix layer; taking out a branch mold and removing a shell mold in layers from inwards to outwards; pouring macromolecular solutions of different types into gaps remained in the shell mold respectively so as to form a multilayered synthetic macromolecule shell; and removing the combined mold and abutting two formed structures so as to obtain the complex organ precursor. According to the method, a complex organ precursor three-dimensional structure, in which a branch vessel network is provided and cellular matrix materials of different types and synthetic macromolecule support shells are combined, is formed; and the defects that the tissue engineering is simple in three-dimensional support surface and the internal structure, low in similarity with real organs, single in cell type and uneven in cell distribution, less in possibility for survival of closed cells, difficult in non-channel differentiation vessels and strong in support appearance limitation are solved.

Description

technical field [0001] The invention belongs to the technical field of artificial manufacturing of biological tissues and organs, in particular to a process for preparing tissue and organ precursors by using synthetic polymer materials and cell matrix materials, and belongs to the technical field of biological tissue engineering. Background technique [0002] Tens of millions of patients suffer from tissue defect or organ failure every year in the world. However, living donor organs are limited, and existing mechanical devices do not have all the functions of the organs and cannot prevent further deterioration of the patient's condition. Accordingly, the tissue engineering (Tissue Engineering) technology for the purpose of improving the treatment level of such diseases came into being. [0003] Tissue engineering was officially proposed and determined by the National Science Foundation of the United States in 1987. It is the application of the principles of cell biology, bi...

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for preparing complex organ precursors with branched vascular networks, aiming to achieve accurate positioning of various cells and scaffold materials in space by using a combined mold for step-by-step perfusion on the basis of previous work. Forming a composite tissue and organ precursor of multiple cells and multiple growth factors wrapped in a multi-layer polymer material shell; using the principles of mold combination and polymer coagulation forming to realize the reconstruction of complex tissues and organs, the invention can form The complex three-dimensional structure containing different cell matrix materials, branched vascular channels and multi-layer synthetic polymer shell overcomes the simple surface and internal structure of the three-dimensional scaffold in tissue engineering, low similarity to real organs, single cell types and uneven distribution, Closed cells are not easy to survive, it is difficult to differentiate blood vessels without channels, and the shape of the stent is limited, etc.

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