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Hydrolysis technology for protease of heparin sodium

A technology of protease hydrolysis and heparin sodium, which is applied in the field of protease hydrolysis of heparin sodium, can solve the problems of slow speed and low extraction rate of heparin sodium, and achieve the effects of large waste of raw materials, high yield and improved production efficiency

Inactive Publication Date: 2012-08-15
RUGAO BAXIN CASING
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

In the body, it combines with protein to form a complex and exists in mast cells, which can be dissociated by protease. Therefore, the current heparin sodium in the small intestine of animals is generally produced by protease hydrolysis. However, due to the simple hydrolysis process The obtained heparin sodium is slow and the extraction rate is low, and this technology needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Select 500g of porcine small intestinal mucosal fluid with a salinity of 4 degrees, add NAOH, adjust its pH to 9, stir and heat up to 55°C, keep warm for 30 minutes, add alkali according to the small intestinal mucosa:alkaline protease weight ratio of 500:0.1 Protease, keep warm for 2 hours, after adding alkaline protease, intervene in ultrasound, specifically: the wavelength of ultrasound is 25Hz, and the power density is 0.5-0.7 W / cm 2 The supersonic wave was intervened once at the beginning and end of the enzymatic hydrolysis, and each treatment was 0.5h. After enzymolysis, conventional resin adsorption, elution, alcohol lake, and drying are used to obtain heparin sodium with a yield of 90% and a potency of 105U / mg. In this production, due to the reduction of the amount of NaCl, the amount of wastewater The reduction of 200 g per 1T of small intestine is achieved, which achieves the production goals of high efficiency, high yield and environmental protection.

Embodiment 2

[0011] Pump 200 pieces of pig small intestine mucosa and water with a salinity of 3.5 degrees in a 1000-liter reactor. The weight ratio of pig small intestine and water is 3:1. Add NAOH to adjust its pH to 9, stir and heat up to 55°C , after 30 minutes of heat preservation, according to the small intestinal mucosa: alkaline protease weight ratio of 500g: 0.1, add alkaline protease and keep warm for 2 hours. W / cm 2 The supersonic wave was intervened once at the beginning and end of the enzymatic hydrolysis, and each treatment was 0.5h. After enzymolysis, conventional resin adsorption, elution, alcohol lake, and drying are used to obtain heparin sodium with a yield of 91% and a potency of 106U / mg. In this production, due to the reduction of the amount of NaCl, the amount of wastewater The reduction of 300g per 1T small intestine is achieved, achieving the production goals of high efficiency, high yield and environmental protection.

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Abstract

The invention discloses a hydrolysis technology for protease of heparin sodium, which is mainly used for extracting heparin sodium by hydrolyzing protein by protease. The hydrolysis technology is characterized by comprising the following steps of: controlling the content of the mucous membrane of small intestine to be 2.5-4% of the weight of the mucous membrane of small intestine when the mucous membrane of small intestine is processed, adding alkaline agent, and adjusting the pH value of the alkaline agent to be 9; and when the heparin sodium is extracted by hydrolyzing the protein by the protease, supplementarily using ultrasonic wave. The hydrolysis technology mainly adopts the intervention of the ultrasonic wave, when the ultrasonic wave is acted on to liquid medium, not only can the cavitation effect and the heat effect be generated and the momentum be given to the liquid molecule, but also the influence of the mass and heat transferring speed to the solubility and the solution rate of the solute can be increased, the yield of the heparin sodium can be improved, and the defect that the production efficiency is reduced caused by lower salinity can be overcome. The production mode of being high in yield, high in efficiency, green, environment-friendly, and less in wastewater discharge can be realized.

Description

technical field [0001] The main task of the present invention is to provide a protease hydrolysis process of heparin sodium, specifically a protease hydrolysis process of heparin sodium obtained by hydrolyzing protein with protease to obtain heparin sodium. Background technique [0002] Heparin sodium is a mucopolysaccharide produced by mast cells of animal connective tissue. It is a natural anticoagulant substance. Treat thromboembolism, fulminant meningitis, sepsis, nephritis, acute myocardial infarction, arteriosclerosis and other diseases. It also has the functions of clarifying plasma lipids and lowering cholesterol. In the body, it combines with protein to form a complex and exists in mast cells, which can be dissociated by protease. Therefore, the current heparin sodium in the small intestine of animals is generally produced by protease hydrolysis. However, due to the simple hydrolysis process The obtained heparin sodium is slow and the extraction rate is low, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10A61P3/06A61P7/02A61P9/10A61P13/12A61P31/04
Inventor 陆海笑顾永军
Owner RUGAO BAXIN CASING
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