Unlock instant, AI-driven research and patent intelligence for your innovation.

Bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide and use thereof

A technology of sf-628 and prochloraz manganese salt, which is applied in the direction of fungicides, applications, biocides, etc., can solve the problems of excessive pesticide residues and pollution of the ecological environment, so as to reduce the cost of medication, improve the ecological environment, and reduce the amount of residues Effect

Inactive Publication Date: 2013-10-30
JIANGSU ACAD OF AGRI SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Another purpose of the present invention is: in view of the problem that a large amount of chemical pesticides are used in current production to prevent and control vegetable diseases, seriously pollute the ecological environment, and cause pesticide residues in agricultural products to exceed the standard. Bacillus subtilis sf-628 and prochloraz manganese salt compound wettable fungicide powder with good control effect and its application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide and use thereof
  • Bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide and use thereof
  • Bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Inhibition of Bacillus subtilis sf-628 against Fusarium wilt and Ralstonia solanacearum.

[0018] Pathogen strains tested:

[0019] Fusarium wilt of tomato was provided by Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. Use PSA medium (200g potato, 20g sucrose, 20g agar, and 1000mL distilled water, pH 5.6-6.6) to culture at 28°C for 5 days, and set aside.

[0020] Tomato solanacearum was provided by the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences. Use NA medium (3.0 g of beef extract, 1 g of yeast extract, 5 g of peptone, 10 g of glucose, 20 g of agar, and 1000 mL of distilled water) at 28°C for 2 days with shaking (150 r / m), and set aside.

[0021] The Bacillus subtilis sf-628 screened by Jiangsu Academy of Agricultural Sciences was activated on NA medium, then transferred into 50 mL NA culture medium, cultured at 28° C. with shaking (150 r / m) for 2 days, and set aside. Place the Fusarium wilt bacteria block in the ...

Embodiment 2

[0027] Toxicity determination of different ratios of Bacillus subtilis sf-628 and prochloraz manganese salts against Fusarium wilt and Ralstonia solanacearum

[0028] (1) For the test bacteria:

[0029] Fusarium wilt of tomato and Ralstonia solanacearum were used as the test bacteria (provided by the Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences).

[0030] (2) Drugs for testing:

[0031] A. 50% prochloraz manganese salt EC was produced and provided by Jiangsu Huifeng Agrochemical Co., Ltd.

[0032] B. 20 billion Bacillus subtilis sf-628 wettable powder is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

[0033] Indoor synergistic bioassay:

[0034] Mix 50% prochloraz manganese salt EC into 0.01μg / mL, 0.1μg / mL, 1μg / mL, 10μg / mL PSA medium plate with medicine; mix 20 billion Bacillus subtilis sf-628 wettable powder into 2×10 6 cfu Bacillus subtilis / mL, 2×10 5 cfu Bacillus subtilis / mL, 2×10 4 cfu Bacillus subtilis / mL, 2×10 3 cfu Bacillu...

Embodiment 3

[0045] Preparation of 15% prochloraz manganese salt·6 billion bacillus subtilis sf-628 wettable powder

[0046] Effective drugs:

[0047] A. 50% prochloraz manganese salt EC was produced and provided by Jiangsu Huifeng Agrochemical Co., Ltd.

[0048] B. 20 billion Bacillus subtilis sf-628 wettable powder is produced and provided by Jiangsu Suke Agrochemical Co., Ltd.

[0049] The auxiliary agent is NNO (sodium methylene dinaphthalene sulfonate); the binder is polyethylene glycol; the surfactant is sodium dodecylbenzene sulfonate; the filler is light calcium carbonate.

[0050] Each component is prepared according to the following weight percentages:

[0051] 50% prochloraz manganese salt: 30%; 20 billion Bacillus subtilis sf-628 wettable powder: 30%; NNO (sodium methylene dinaphthalene sulfonate): 7%; polyethylene glycol: 3%; ten Sodium Dialkylbenzene Sulfonate: 5%; Light Calcium Carbonate: 25%.

[0052] Preparation process: put prochloraz manganese salt, Bacillus subtilis...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide and a use thereof. The bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide contains active components of bacillus subtilis sf-628 and a prochloraz-manganese chloride complex. The bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide is characterized in that a weight ratio of the active components of bacillus subtilis sf-628 to the prochloraz-manganese chloride complex is (1-99): (99-1); the bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide is wettable powder and comprises 30% of the prochloraz-manganese chloride complex having the concentration of 50%, 30% of wettable powder of 20 billion bacillus subtilis sf-628, 7% of NNO (disodium methylene dinaphthalenesulfonate), 3% of polyethylene glycol, 5% of sodium dodecyl benzene sulfonate and 25% of light calcium carbonate. In use, the bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide is diluted and then is injected into soil located at the root of a vegetable so that soil-borne diseases of vegetables are prevented and treated, wherein the soil-borne diseases mainly comprise fusarium wilt and bacterial wilt of solanaceous fruit vegetables, and the diluted bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide has effective bactericide content of at least 300mg / L. The bacillus subtilis sf-628 and prochloraz-manganese chloride complex compounded bactericide has the advantages that bacillus subtilis sf-628 and a chemical agent prochloraz-manganese chloride complex is mixed according to a certain ratio so that advantages are complementary to each other; use amounts of chemical agents are reduced; a bactericide use cost is reduced; prevention and treatment effects are improved; ecological environment is improved; and the residual amount of a chemical agent in an agricultural product is reduced.

Description

technical field [0001] The present invention relates to a compound fungicide and its application, especially the fungicide compounded by a strain of Bacillus subtilis sf-628 and prochloraz manganese salt and its application in preventing and treating soil-borne diseases of solanaceous crops (fusarium wilt and bacterial wilt) application. Background technique [0002] With the improvement of people's living standards, the requirements for food safety are getting higher and higher. Ensuring the supply of pollution-free and high-quality agricultural products and realizing the sustainable use of resources are the biggest challenges facing agricultural production in the 21st century. On the one hand, a substantial increase in the planting area of ​​protected vegetables is the core content of the structural adjustment of the planting industry; on the other hand, the high-yield, continuous cropping, and diversified planting layout of protected vegetables provides sufficient nutrit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01N59/16A01N47/38A01P3/00
Inventor 刘邮洲陈志谊刘永锋聂亚峰乔俊卿
Owner JIANGSU ACAD OF AGRI SCI