Method for extracting and refining rutin

Abstract

A method for extracting and refining rutin comprises the following steps of (1) extraction: smashing raw materials, adding organic solvent into the smashed raw materials, performing extraction for 2-4 times, mixing extracting solution and concentrating the mixed extracting solution to thick extract with organic solvent content smaller than or equal to 5wt%; (2) alkali dissolution-acid sediment: adding alkaline water into the thick extract, then heating the mixture and stirring, filtering the heated mixture, regulating pH of alkaline filtrate to be 3-4, standing, filtering, drying to obtain a rutin pure product; and (3) mother liquor treatment: enabling obtained acid mother liquor to penetrate through a chromatographic column filled with resin, after the acid mother liquor penetrates through the chromatographic column, firstly using deionized water to wash a column bed until outflow liquid is neutral and colorless, introducing eluent into the chromatographic column and collecting eluate until outflow liquid is colorless, concentrating the eluate to the volume which is 5-10% of the original volume in a pressure reduction mode, cooling the concentrated eluate to achieve crystallization, filtering, using the deionized water to elute a filter cake, and drying the eluted filter cake to obtain a mother liquor rutin product. The prepared rutin is high in yield and purity, and the quality of the rutin reaches the international standard.

Description

technical field [0001] The invention relates to a method for extracting and refining rutin. Background technique [0002] Rutin (Rutin), which belongs to flavonoids, is also known as rutin, vitamin P, and purple quercetin; it not only reduces the permeability and fragility of capillaries, but also maintains and restores the normal elasticity of capillaries. It is used to prevent and treat hypertensive cerebral hemorrhage, diabetic retinal hemorrhage and hemorrhagic purpura. [0003] At present, the methods for producing rutin mainly include: 1, hot water extraction method, although this method is simple in process and low in equipment requirements, but the product yield is low and the quality is poor; 2, alkali extraction-acid precipitation method, this method prepares rutin Although the product quality is slightly better, the yield is on the low side; 3, solvent extraction method, although this method yield is guaranteed, the refining of the product is quite difficult, and...

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for producing rutin mainly include: 1, hot water extraction method, although this method is simple in process and low in equipment requirements, but the product yield is low and the quality is poor; 2, alkali extraction-acid precipitation method, this method prepares rutin Although the quality of the product is slightly better, the yield is low; 3. Solvent extraction method, although the yield of this method is guaranteed, the refining of the product is quite difficult, and the product content is low

It can be seen that the above methods have their own advantages and disadvantages.